Abstract
We investigated the expression, degree of phosphorylation, and activation of the proto-oncogene c-kit product before and after stimulation with the c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E. The culture supernatant of the BALB/3T3 fibroblast cell line, which contains the ligand for the murine c-kit product, was found to stimulate proliferation of the MO7E cell line in a dose-dependent manner. The proliferation was significantly inhibited by a tyrosine kinase inhibitor, genistein. An immunoblot technique with a monoclonal antibody specific for phosphotyrosine, showed that there was rapid, dose-dependent tyrosine-phosphorylation of the c-kit product in response to murine c-kit ligand. Furthermore, the murine c-kit ligand increased autokinase activity of the c-kit product in vitro. Similar results were obtained with human stem cell factor (SCF), a recombinant human ligand for the c-kit product. These results suggest that the phosphorylation and activation of the c-kit product are involved in proliferative signals of some human leukemia cells, as well as of normal hematopoietic cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2834-2840 |
| Number of pages | 7 |
| Journal | Blood |
| Volume | 78 |
| Issue number | 11 |
| State | Published - Dec 1 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product. / Kuriu, Akira; Ikeda, Hirokazu; Kanakura, Yuzuru; Griffin, James D.; Druker, Brian; Yagura, Hirosuke; Kitayama, Hitoshi; Ishikawa, Jun; Nishiura, Tetsuo; Kanayama, Yoshio; Yonezawa, Takeshi; Tarui, Seiichiro.
In: Blood, Vol. 78, No. 11, 01.12.1991, p. 2834-2840.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product
AU - Kuriu, Akira
AU - Ikeda, Hirokazu
AU - Kanakura, Yuzuru
AU - Griffin, James D.
AU - Druker, Brian
AU - Yagura, Hirosuke
AU - Kitayama, Hitoshi
AU - Ishikawa, Jun
AU - Nishiura, Tetsuo
AU - Kanayama, Yoshio
AU - Yonezawa, Takeshi
AU - Tarui, Seiichiro
PY - 1991/12/1
Y1 - 1991/12/1
N2 - We investigated the expression, degree of phosphorylation, and activation of the proto-oncogene c-kit product before and after stimulation with the c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E. The culture supernatant of the BALB/3T3 fibroblast cell line, which contains the ligand for the murine c-kit product, was found to stimulate proliferation of the MO7E cell line in a dose-dependent manner. The proliferation was significantly inhibited by a tyrosine kinase inhibitor, genistein. An immunoblot technique with a monoclonal antibody specific for phosphotyrosine, showed that there was rapid, dose-dependent tyrosine-phosphorylation of the c-kit product in response to murine c-kit ligand. Furthermore, the murine c-kit ligand increased autokinase activity of the c-kit product in vitro. Similar results were obtained with human stem cell factor (SCF), a recombinant human ligand for the c-kit product. These results suggest that the phosphorylation and activation of the c-kit product are involved in proliferative signals of some human leukemia cells, as well as of normal hematopoietic cells.
AB - We investigated the expression, degree of phosphorylation, and activation of the proto-oncogene c-kit product before and after stimulation with the c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E. The culture supernatant of the BALB/3T3 fibroblast cell line, which contains the ligand for the murine c-kit product, was found to stimulate proliferation of the MO7E cell line in a dose-dependent manner. The proliferation was significantly inhibited by a tyrosine kinase inhibitor, genistein. An immunoblot technique with a monoclonal antibody specific for phosphotyrosine, showed that there was rapid, dose-dependent tyrosine-phosphorylation of the c-kit product in response to murine c-kit ligand. Furthermore, the murine c-kit ligand increased autokinase activity of the c-kit product in vitro. Similar results were obtained with human stem cell factor (SCF), a recombinant human ligand for the c-kit product. These results suggest that the phosphorylation and activation of the c-kit product are involved in proliferative signals of some human leukemia cells, as well as of normal hematopoietic cells.
UR - http://www.scopus.com/inward/record.url?scp=0025718733&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025718733&partnerID=8YFLogxK
M3 - Article
VL - 78
SP - 2834
EP - 2840
JO - Blood
JF - Blood
SN - 0006-4971
IS - 11
ER -