Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product

A. Kuriu, H. Ikeda, Y. Kanakura, J. D. Griffin, B. Druker, H. Yagura, H. Kitayama, J. Ishikawa, T. Nishiura, Y. Kanayama, T. Yonezawa, S. Tarui

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

We investigated the expression, degree of phosphorylation, and activation of the proto-oncogene c-kit product before and after stimulation with the c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E. The culture supernatant of the BALB/3T3 fibroblast cell line, which contains the ligand for the murine c-kit product, was found to stimulate proliferation of the MO7E cell line in a dose-dependent manner. The proliferation was significantly inhibited by a tyrosine kinase inhibitor, genistein. An immunoblot technique with a monoclonal antibody specific for phosphotyrosine, showed that there was rapid, dose-dependent tyrosine-phosphorylation of the c-kit product in response to murine c-kit ligand. Furthermore, the murine c-kit ligand increased autokinase activity of the c-kit product in vitro. Similar results were obtained with human stem cell factor (SCF), a recombinant human ligand for the c-kit product. These results suggest that the phosphorylation and activation of the c-kit product are involved in proliferative signals of some human leukemia cells, as well as of normal hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)2834-2840
Number of pages7
JournalBlood
Volume78
Issue number11
StatePublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product'. Together they form a unique fingerprint.

  • Cite this

    Kuriu, A., Ikeda, H., Kanakura, Y., Griffin, J. D., Druker, B., Yagura, H., Kitayama, H., Ishikawa, J., Nishiura, T., Kanayama, Y., Yonezawa, T., & Tarui, S. (1991). Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product. Blood, 78(11), 2834-2840.