Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product

Akira Kuriu, Hirokazu Ikeda, Yuzuru Kanakura, James D. Griffin, Brian Druker, Hirosuke Yagura, Hitoshi Kitayama, Jun Ishikawa, Tetsuo Nishiura, Yoshio Kanayama, Takeshi Yonezawa, Seiichiro Tarui

Research output: Contribution to journalArticle

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Abstract

We investigated the expression, degree of phosphorylation, and activation of the proto-oncogene c-kit product before and after stimulation with the c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E. The culture supernatant of the BALB/3T3 fibroblast cell line, which contains the ligand for the murine c-kit product, was found to stimulate proliferation of the MO7E cell line in a dose-dependent manner. The proliferation was significantly inhibited by a tyrosine kinase inhibitor, genistein. An immunoblot technique with a monoclonal antibody specific for phosphotyrosine, showed that there was rapid, dose-dependent tyrosine-phosphorylation of the c-kit product in response to murine c-kit ligand. Furthermore, the murine c-kit ligand increased autokinase activity of the c-kit product in vitro. Similar results were obtained with human stem cell factor (SCF), a recombinant human ligand for the c-kit product. These results suggest that the phosphorylation and activation of the c-kit product are involved in proliferative signals of some human leukemia cells, as well as of normal hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)2834-2840
Number of pages7
JournalBlood
Volume78
Issue number11
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Phosphorylation
Stem Cell Factor
Myeloid Leukemia
Proto-Oncogenes
Myeloid Cells
Tyrosine
Chemical activation
Cells
Cell Line
BALB 3T3 Cells
Phosphotyrosine
Genistein
Fibroblasts
Human engineering
Cell culture
Protein-Tyrosine Kinases
Monoclonal Antibodies
Leukemia
Ligands

ASJC Scopus subject areas

  • Hematology

Cite this

Kuriu, A., Ikeda, H., Kanakura, Y., Griffin, J. D., Druker, B., Yagura, H., ... Tarui, S. (1991). Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product. Blood, 78(11), 2834-2840.

Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product. / Kuriu, Akira; Ikeda, Hirokazu; Kanakura, Yuzuru; Griffin, James D.; Druker, Brian; Yagura, Hirosuke; Kitayama, Hitoshi; Ishikawa, Jun; Nishiura, Tetsuo; Kanayama, Yoshio; Yonezawa, Takeshi; Tarui, Seiichiro.

In: Blood, Vol. 78, No. 11, 01.12.1991, p. 2834-2840.

Research output: Contribution to journalArticle

Kuriu, A, Ikeda, H, Kanakura, Y, Griffin, JD, Druker, B, Yagura, H, Kitayama, H, Ishikawa, J, Nishiura, T, Kanayama, Y, Yonezawa, T & Tarui, S 1991, 'Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product', Blood, vol. 78, no. 11, pp. 2834-2840.
Kuriu, Akira ; Ikeda, Hirokazu ; Kanakura, Yuzuru ; Griffin, James D. ; Druker, Brian ; Yagura, Hirosuke ; Kitayama, Hitoshi ; Ishikawa, Jun ; Nishiura, Tetsuo ; Kanayama, Yoshio ; Yonezawa, Takeshi ; Tarui, Seiichiro. / Proliferation of human myeloid leukemia cell line associated with the tyrosine-phosphorylation and activation of the proto-oncogene c-kit product. In: Blood. 1991 ; Vol. 78, No. 11. pp. 2834-2840.
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