Prolactin receptor expression by rat NK cells

W. H. Chambers, A. A. Amoscato, M (Susan) Smith, T. W. Kenniston, R. B. Herberman, P. M. Appasamy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have recently begun investigating the effects of prolactin (PRL) on the function, or growth and differentiation of rat NK cells. Initially, we sought to determine the expression of a receptor for PRL (PRLr) on rat NK cells and to determine the effects of interleukin 2 (IL-2) on its expression. By a combination of reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting, we were able to identify a PRLr specific transcript using RNA isolated from highly purified, IL-2-activated NK (A-NK) cells. Using primers specific for unique portions of the major forms of PRLr transcripts in liver and ovary, it was determined that the NK cell PRLr has some homology with the major form of transcript for PRLr found in rat liver. By flow cytometric and 125I-PRL binding analyses, we determined that A-NK cells expressed detectable levels of PRLr internally. Surface PRLr was not detectable by flow cytometry on freshly isolated NK cells from normal rats, and incubation of NK cells with rIL-2 did not induce detectable surface PRLr. Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL2rα; CD25) on NK cells or other lymphoid cells. However, we were able to detect surface expression of PRLr on NK cells from bromocriptine-treated rats. Taken together, these data indicate that both cultured and freshly isolated populations of NK cell express PRLr.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalNatural Immunity
Volume14
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Prolactin Receptors
Natural Killer Cells
Prolactin
Interleukin-2
Interleukin-2 Receptor alpha Subunit
Bromocriptine
Liver
Southern Blotting
Reverse Transcription
Ovary
Flow Cytometry

Keywords

  • Natural killer cells
  • Prolactin
  • Prolactin receptor

ASJC Scopus subject areas

  • Immunology

Cite this

Chambers, W. H., Amoscato, A. A., Smith, M. S., Kenniston, T. W., Herberman, R. B., & Appasamy, P. M. (1995). Prolactin receptor expression by rat NK cells. Natural Immunity, 14(3), 145-156.

Prolactin receptor expression by rat NK cells. / Chambers, W. H.; Amoscato, A. A.; Smith, M (Susan); Kenniston, T. W.; Herberman, R. B.; Appasamy, P. M.

In: Natural Immunity, Vol. 14, No. 3, 1995, p. 145-156.

Research output: Contribution to journalArticle

Chambers, WH, Amoscato, AA, Smith, MS, Kenniston, TW, Herberman, RB & Appasamy, PM 1995, 'Prolactin receptor expression by rat NK cells', Natural Immunity, vol. 14, no. 3, pp. 145-156.
Chambers WH, Amoscato AA, Smith MS, Kenniston TW, Herberman RB, Appasamy PM. Prolactin receptor expression by rat NK cells. Natural Immunity. 1995;14(3):145-156.
Chambers, W. H. ; Amoscato, A. A. ; Smith, M (Susan) ; Kenniston, T. W. ; Herberman, R. B. ; Appasamy, P. M. / Prolactin receptor expression by rat NK cells. In: Natural Immunity. 1995 ; Vol. 14, No. 3. pp. 145-156.
@article{a752149fc3a241fb979e1cf8f2dc3442,
title = "Prolactin receptor expression by rat NK cells",
abstract = "We have recently begun investigating the effects of prolactin (PRL) on the function, or growth and differentiation of rat NK cells. Initially, we sought to determine the expression of a receptor for PRL (PRLr) on rat NK cells and to determine the effects of interleukin 2 (IL-2) on its expression. By a combination of reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting, we were able to identify a PRLr specific transcript using RNA isolated from highly purified, IL-2-activated NK (A-NK) cells. Using primers specific for unique portions of the major forms of PRLr transcripts in liver and ovary, it was determined that the NK cell PRLr has some homology with the major form of transcript for PRLr found in rat liver. By flow cytometric and 125I-PRL binding analyses, we determined that A-NK cells expressed detectable levels of PRLr internally. Surface PRLr was not detectable by flow cytometry on freshly isolated NK cells from normal rats, and incubation of NK cells with rIL-2 did not induce detectable surface PRLr. Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL2rα; CD25) on NK cells or other lymphoid cells. However, we were able to detect surface expression of PRLr on NK cells from bromocriptine-treated rats. Taken together, these data indicate that both cultured and freshly isolated populations of NK cell express PRLr.",
keywords = "Natural killer cells, Prolactin, Prolactin receptor",
author = "Chambers, {W. H.} and Amoscato, {A. A.} and Smith, {M (Susan)} and Kenniston, {T. W.} and Herberman, {R. B.} and Appasamy, {P. M.}",
year = "1995",
language = "English (US)",
volume = "14",
pages = "145--156",
journal = "Natural Immunity",
issn = "1018-8916",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Prolactin receptor expression by rat NK cells

AU - Chambers, W. H.

AU - Amoscato, A. A.

AU - Smith, M (Susan)

AU - Kenniston, T. W.

AU - Herberman, R. B.

AU - Appasamy, P. M.

PY - 1995

Y1 - 1995

N2 - We have recently begun investigating the effects of prolactin (PRL) on the function, or growth and differentiation of rat NK cells. Initially, we sought to determine the expression of a receptor for PRL (PRLr) on rat NK cells and to determine the effects of interleukin 2 (IL-2) on its expression. By a combination of reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting, we were able to identify a PRLr specific transcript using RNA isolated from highly purified, IL-2-activated NK (A-NK) cells. Using primers specific for unique portions of the major forms of PRLr transcripts in liver and ovary, it was determined that the NK cell PRLr has some homology with the major form of transcript for PRLr found in rat liver. By flow cytometric and 125I-PRL binding analyses, we determined that A-NK cells expressed detectable levels of PRLr internally. Surface PRLr was not detectable by flow cytometry on freshly isolated NK cells from normal rats, and incubation of NK cells with rIL-2 did not induce detectable surface PRLr. Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL2rα; CD25) on NK cells or other lymphoid cells. However, we were able to detect surface expression of PRLr on NK cells from bromocriptine-treated rats. Taken together, these data indicate that both cultured and freshly isolated populations of NK cell express PRLr.

AB - We have recently begun investigating the effects of prolactin (PRL) on the function, or growth and differentiation of rat NK cells. Initially, we sought to determine the expression of a receptor for PRL (PRLr) on rat NK cells and to determine the effects of interleukin 2 (IL-2) on its expression. By a combination of reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting, we were able to identify a PRLr specific transcript using RNA isolated from highly purified, IL-2-activated NK (A-NK) cells. Using primers specific for unique portions of the major forms of PRLr transcripts in liver and ovary, it was determined that the NK cell PRLr has some homology with the major form of transcript for PRLr found in rat liver. By flow cytometric and 125I-PRL binding analyses, we determined that A-NK cells expressed detectable levels of PRLr internally. Surface PRLr was not detectable by flow cytometry on freshly isolated NK cells from normal rats, and incubation of NK cells with rIL-2 did not induce detectable surface PRLr. Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL2rα; CD25) on NK cells or other lymphoid cells. However, we were able to detect surface expression of PRLr on NK cells from bromocriptine-treated rats. Taken together, these data indicate that both cultured and freshly isolated populations of NK cell express PRLr.

KW - Natural killer cells

KW - Prolactin

KW - Prolactin receptor

UR - http://www.scopus.com/inward/record.url?scp=0029565196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029565196&partnerID=8YFLogxK

M3 - Article

C2 - 8832898

AN - SCOPUS:0029565196

VL - 14

SP - 145

EP - 156

JO - Natural Immunity

JF - Natural Immunity

SN - 1018-8916

IS - 3

ER -