Prolactin receptor expression by rat NK cells

W. H. Chambers, A. A. Amoscato, M. S. Smith, T. W. Kenniston, R. B. Herberman, P. M. Appasamy

    Research output: Contribution to journalArticle

    15 Scopus citations

    Abstract

    We have recently begun investigating the effects of prolactin (PRL) on the function, or growth and differentiation of rat NK cells. Initially, we sought to determine the expression of a receptor for PRL (PRLr) on rat NK cells and to determine the effects of interleukin 2 (IL-2) on its expression. By a combination of reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting, we were able to identify a PRLr specific transcript using RNA isolated from highly purified, IL-2-activated NK (A-NK) cells. Using primers specific for unique portions of the major forms of PRLr transcripts in liver and ovary, it was determined that the NK cell PRLr has some homology with the major form of transcript for PRLr found in rat liver. By flow cytometric and 125I-PRL binding analyses, we determined that A-NK cells expressed detectable levels of PRLr internally. Surface PRLr was not detectable by flow cytometry on freshly isolated NK cells from normal rats, and incubation of NK cells with rIL-2 did not induce detectable surface PRLr. Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL2rα; CD25) on NK cells or other lymphoid cells. However, we were able to detect surface expression of PRLr on NK cells from bromocriptine-treated rats. Taken together, these data indicate that both cultured and freshly isolated populations of NK cell express PRLr.

    Original languageEnglish (US)
    Pages (from-to)145-156
    Number of pages12
    JournalNatural Immunity
    Volume14
    Issue number3
    StatePublished - Dec 1 1995

    Keywords

    • Natural killer cells
    • Prolactin
    • Prolactin receptor

    ASJC Scopus subject areas

    • Immunology

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  • Cite this

    Chambers, W. H., Amoscato, A. A., Smith, M. S., Kenniston, T. W., Herberman, R. B., & Appasamy, P. M. (1995). Prolactin receptor expression by rat NK cells. Natural Immunity, 14(3), 145-156.