Progesterone promotes oocyte maturation, but not ovulation, in nonhuman primate follicles without a gonadotropin surge

Sherri M. Borman, Charles L. Chaffin, Kristine M. Schwinof, Richard Stouffer, Mary Zelinski

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    During the periovulatory interval, intrafollicular progesterone (P) prevents follicular atresia and promotes ovulation. Whether P influences oocyte quality or maturation and follicle rupture independent of the midcycle gonadotropin surge was examined. Rhesus monkeys underwent controlled ovarian stimulation with recombinant human gonadotropins followed by a) experiment 1: an ovulatory bolus of hCG alone or with a steroid synthesis inhibitor (trilostane, TRL), or TRL + the progestin R5020; or b) no hCG, but rather sesame oil (vehicle), R5020, or dihydrotestosterone (DHT). In experiment 1, the majority of oocytes remained immature (65% ± 20%) by 12 h post-hCG. However, the percentage of degenerating oocytes increased (P <0.05) with TRL (42% ± 22% vs. 0% controls), but was reduced (P <0.05) by progestin replacement (15% ± 7%). By 36 h post-hCG, the majority of oocytes in all three groups reached metaphase II (MI). In experiment 2, no evidence of follicle rupture was observed in the vehicle, R5020, of DHT groups. Despite the absence of hCG, a significant (P <0.05) percentage of oocytes resumed meiosis to metaphase I in R5020- (41 ± 9) and DHT- (36 ± 15) but not vehicle- (4 ± 4) treated animals. Only oocytes from R5020-treated animals continued meiosis in vivo to MII. More (P <0.05) oocytes fertilized in vitro with R5020 (40%) than with vehicle (20%) or DHT (22%). Thus, P is unable to elicit ovulation in the absence of an ovulatory gonadotropin surge; however, P and/or androgens may prevent oocyte atresia and promote oocyte nuclear maturation in primate follicles.

    Original languageEnglish (US)
    Pages (from-to)366-373
    Number of pages8
    JournalBiology of Reproduction
    Volume71
    Issue number1
    DOIs
    StatePublished - Jul 2004

    Fingerprint

    Ovulation
    Promegestone
    Gonadotropins
    Primates
    Oocytes
    Progesterone
    Dihydrotestosterone
    Meiosis
    Progestins
    Metaphase
    Rupture
    Follicular Atresia
    Sesame Oil
    Ovulation Induction
    Macaca mulatta
    Androgens

    Keywords

    • In vitro fertilization
    • Meiosis
    • Ovulation
    • Ovum
    • Progesterone

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Progesterone promotes oocyte maturation, but not ovulation, in nonhuman primate follicles without a gonadotropin surge. / Borman, Sherri M.; Chaffin, Charles L.; Schwinof, Kristine M.; Stouffer, Richard; Zelinski, Mary.

    In: Biology of Reproduction, Vol. 71, No. 1, 07.2004, p. 366-373.

    Research output: Contribution to journalArticle

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    AU - Stouffer, Richard

    AU - Zelinski, Mary

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