Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates

Katie R. Tallman, Samantha R. Levine, Kimberly Beatty

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Enzyme-activated, fluorogenic probes are powerful tools for studying bacterial pathogens, including Mycobacterium tuberculosis (Mtb). In prior work, we reported two 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO)-derived acetoxymethyl ether probes for esterase and lipase detection. Here, we report four-carbon (C4) and eight-carbon (C8) acyloxymethyl ether derivatives, which are longer-chain fluorogenic substrates. These new probes demonstrate greater stability and lipase reactivity than the two-carbon (C2) acetoxymethyl ether-masked substrates. We used these new C4 and C8 probes to profile esterases and lipases from Mtb. The C8-masked probes revealed a new esterase band in gel-resolved Mtb lysates that was not present in lysates from nonpathogenic M. bovis (bacillus Calmette-Guérin), a close genetic relative. We identified this Mtb-specific enzyme as the secreted esterase Culp1 (Rv1984c). Our C4- and C8-masked probes also produced distinct Mtb banding patterns in lysates from Mtb-infected macrophages, demonstrating the potential of these probes for detecting Mtb esterases that are active during infections.

Original languageEnglish (US)
Pages (from-to)1810-1815
Number of pages6
JournalACS Chemical Biology
Volume11
Issue number7
DOIs
StatePublished - Jul 15 2016

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Esterases
Fluorescent Dyes
Mycobacterium tuberculosis
Lipase
Ether
Carbon
Macrophages
Pathogens
Bacilli
Enzymes
Bacillus
Gels
Derivatives
Substrates
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates. / Tallman, Katie R.; Levine, Samantha R.; Beatty, Kimberly.

In: ACS Chemical Biology, Vol. 11, No. 7, 15.07.2016, p. 1810-1815.

Research output: Contribution to journalArticle

Tallman, Katie R. ; Levine, Samantha R. ; Beatty, Kimberly. / Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates. In: ACS Chemical Biology. 2016 ; Vol. 11, No. 7. pp. 1810-1815.
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