Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor

R. G. Rosenfeld; H. Pham

doi:10.1016/0006-291X(87)90588-2

Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor

R. G. Rosenfeld, H. Pham

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

BALB c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.

Original language	English (US)
Pages (from-to)	717-724
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)90588-2
State	Published - Jul 31 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)90588-2

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title = "Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor",

abstract = "BALB c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.",

author = "Rosenfeld, {R. G.} and H. Pham",

note = "Funding Information: supported by NIH grants DK28229 (RGR) and DK36054 (RGR), and by a research award from the Diabetes Research and Education Foundation (RGR). Funding Information: ACKNOWLEDGMENTS: Career Development Dr. Award Rosenfeld from the is NIH the (DK01275). recipient This of a work Research was",

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AU - Rosenfeld, R. G.

AU - Pham, H.

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N2 - BALB c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.

AB - BALB c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.

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Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor

Abstract

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