Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor

Ronald (Ron) Rosenfeld, H. Pham

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BALB c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.

Original languageEnglish (US)
Pages (from-to)717-724
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number2
DOIs
StatePublished - Jul 31 1987
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
Lymphocytes
Cloning
Chromatography
Sepharose
Fusion reactions
Monoclonal Antibodies
Clone Cells
Enzyme-Linked Immunosorbent Assay
Organism Cloning
rat insulin-like growth factor II

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor. / Rosenfeld, Ronald (Ron); Pham, H.

In: Biochemical and Biophysical Research Communications, Vol. 146, No. 2, 31.07.1987, p. 717-724.

Research output: Contribution to journalArticle

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