TY - JOUR
T1 - Production and characterization of a monoclonal antibody to human type IV collagen
AU - Sakai, L. Y.
AU - Engvall, E.
AU - Hollister, D. W.
AU - Burgeson, R. E.
PY - 1982
Y1 - 1982
N2 - We have produced a monoclonal antibody to human basement membrane Type IV collagen. The antibody reacts with the pepsin-resistant, collagenase-sensitive domain of Type IV collagen isolated from placental membranes, but not with human collagens of Types, I, II, III, V, 1α, 2α, and 3α. The antibody precipitates biosynthetically labeled human Type IV procollagen, and the precipitate contains both the α1(IV) and α2(IV) chains, suggesting the occurrence of both of these chains within the same triple-helical molecule. When used in indirect immunofluorescence, the antibody gives brilliant staining of basement membranes from a variety of human tissues but does not stain tissues of bovine, canine, rabbit, rat, or mouse origin. It is suggested that this antibody will be of value in research on the structure of human basement membrane collagen, on the distribution of this collagen in various basement membranes, and particularly for the study of basement membranes in normal human development and pathologic processes.
AB - We have produced a monoclonal antibody to human basement membrane Type IV collagen. The antibody reacts with the pepsin-resistant, collagenase-sensitive domain of Type IV collagen isolated from placental membranes, but not with human collagens of Types, I, II, III, V, 1α, 2α, and 3α. The antibody precipitates biosynthetically labeled human Type IV procollagen, and the precipitate contains both the α1(IV) and α2(IV) chains, suggesting the occurrence of both of these chains within the same triple-helical molecule. When used in indirect immunofluorescence, the antibody gives brilliant staining of basement membranes from a variety of human tissues but does not stain tissues of bovine, canine, rabbit, rat, or mouse origin. It is suggested that this antibody will be of value in research on the structure of human basement membrane collagen, on the distribution of this collagen in various basement membranes, and particularly for the study of basement membranes in normal human development and pathologic processes.
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M3 - Article
C2 - 6287846
AN - SCOPUS:0019958324
SN - 0002-9440
VL - 108
SP - 310
EP - 318
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 3
ER -