Production and characterization of a monoclonal antibody to human type IV collagen

Lynn Sakai, E. Engvall, D. W. Hollister, R. E. Burgeson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have produced a monoclonal antibody to human basement membrane Type IV collagen. The antibody reacts with the pepsin-resistant, collagenase-sensitive domain of Type IV collagen isolated from placental membranes, but not with human collagens of Types, I, II, III, V, 1α, 2α, and 3α. The antibody precipitates biosynthetically labeled human Type IV procollagen, and the precipitate contains both the α1(IV) and α2(IV) chains, suggesting the occurrence of both of these chains within the same triple-helical molecule. When used in indirect immunofluorescence, the antibody gives brilliant staining of basement membranes from a variety of human tissues but does not stain tissues of bovine, canine, rabbit, rat, or mouse origin. It is suggested that this antibody will be of value in research on the structure of human basement membrane collagen, on the distribution of this collagen in various basement membranes, and particularly for the study of basement membranes in normal human development and pathologic processes.

Original languageEnglish (US)
Pages (from-to)310-318
Number of pages9
JournalAmerican Journal of Pathology
Volume108
Issue number3
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Collagen Type IV
Basement Membrane
Monoclonal Antibodies
Antibodies
Collagen
Collagen Type II
Pepsin A
Human Development
Collagenases
Pathologic Processes
Indirect Fluorescent Antibody Technique
Collagen Type I
Canidae
Coloring Agents
Staining and Labeling
Rabbits
Membranes
Research

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sakai, L., Engvall, E., Hollister, D. W., & Burgeson, R. E. (1982). Production and characterization of a monoclonal antibody to human type IV collagen. American Journal of Pathology, 108(3), 310-318.

Production and characterization of a monoclonal antibody to human type IV collagen. / Sakai, Lynn; Engvall, E.; Hollister, D. W.; Burgeson, R. E.

In: American Journal of Pathology, Vol. 108, No. 3, 01.01.1982, p. 310-318.

Research output: Contribution to journalArticle

Sakai, L, Engvall, E, Hollister, DW & Burgeson, RE 1982, 'Production and characterization of a monoclonal antibody to human type IV collagen', American Journal of Pathology, vol. 108, no. 3, pp. 310-318.
Sakai, Lynn ; Engvall, E. ; Hollister, D. W. ; Burgeson, R. E. / Production and characterization of a monoclonal antibody to human type IV collagen. In: American Journal of Pathology. 1982 ; Vol. 108, No. 3. pp. 310-318.
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