Prevalence of Selected Bacterial Named Species and Uncultivated Phylotypes in Endodontic Abscesses From Two Geographic Locations

Isabela N. Rôças, John (Craig) Baumgartner, Tian Xia, José F. Siqueira

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. This study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; Portland, OR, and Rio de Janeiro, Brazil. DNA was extracted from aspirates and used as template in nested PCR assays using 16S rRNA gene taxon-specific oligonucleotide primers. Of the target species/phylotypes, Treponema denticola (73% of the cases), Porphyromonas endodontalis (70%), and Tannerella forsythia (57%) were the most prevalent taxa found in Brazilian samples. Dialister invisus (70% of the cases), P. endodontalis (63%) and Dialister pneumosintes (55%) were the most frequent taxa in the Portland, OR samples. Data analysis revealed that T. denticola and T. forsythia were significantly more detected in Brazilian samples than in the Portland, OR samples. Although D. invisus, Filifactor alocis, and Synergistes oral clone W090 were detected in many more samples from the Portland, OR patients, differences were not found to be statistically significant. These findings confirmed that some bacterial taxa can markedly differ in the frequencies they occur in samples from different locations. It remains to be clarified whether this observation translates into relevant therapeutic implications.

Original languageEnglish (US)
Pages (from-to)1135-1138
Number of pages4
JournalJournal of Endodontics
Volume32
Issue number12
DOIs
StatePublished - Dec 2006

Fingerprint

Porphyromonas endodontalis
Treponema denticola
Geographic Locations
Endodontics
Abscess
DNA Primers
rRNA Genes
Brazil
Clone Cells
Polymerase Chain Reaction
DNA
Tannerella forsythia
Therapeutics

Keywords

  • Acute apical abscess
  • endodontic pathogens
  • geographic location
  • nested PCR

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Prevalence of Selected Bacterial Named Species and Uncultivated Phylotypes in Endodontic Abscesses From Two Geographic Locations. / Rôças, Isabela N.; Baumgartner, John (Craig); Xia, Tian; Siqueira, José F.

In: Journal of Endodontics, Vol. 32, No. 12, 12.2006, p. 1135-1138.

Research output: Contribution to journalArticle

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