Preferential addition of newly synthesized membrane protein at axonal growth cones

Ann Marie Craig, Russell J. Wyborski, Gary Banker

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

THE addition of plasma membrane proteins to a growing axon could occur by preferential insertion at the tip (the growth cone), by uniform insertion along the axon, or by insertion at the cell body and bulk flow along the axon. To differentiate between these possibilities we used a defective herpesvirus vector to express an exogenous protein, the lymphocyte transmembrane protein CD8α, in cultured rat hippocampal neurons. The newly synthesized protein first appeared on the axonal surface almost exclusively at the growth cone. Preferential addition at the growth cone was also observed in minor processes (immature dendrites), but not in mature dendrites. Over several hours, CD8α reached a uniform distribution over the entire neuronal surface, presumably by diffusion within the membrane and possibly endocytic recycling. As well as providing materials for axonal growth, the selective addition of membrane vesicles at the growth cone may contribute to the polarized distribution of axonal surface molecules1.

Original languageEnglish (US)
Pages (from-to)592-594
Number of pages3
JournalNature
Volume375
Issue number6532
StatePublished - 1995
Externally publishedYes

Fingerprint

Growth Cones
Membrane Proteins
Axons
Dendrites
Proteins
Membranes
Herpesviridae
Blood Proteins
Cell Membrane
Lymphocytes
Neurons
Growth

ASJC Scopus subject areas

  • General

Cite this

Craig, A. M., Wyborski, R. J., & Banker, G. (1995). Preferential addition of newly synthesized membrane protein at axonal growth cones. Nature, 375(6532), 592-594.

Preferential addition of newly synthesized membrane protein at axonal growth cones. / Craig, Ann Marie; Wyborski, Russell J.; Banker, Gary.

In: Nature, Vol. 375, No. 6532, 1995, p. 592-594.

Research output: Contribution to journalArticle

Craig, AM, Wyborski, RJ & Banker, G 1995, 'Preferential addition of newly synthesized membrane protein at axonal growth cones', Nature, vol. 375, no. 6532, pp. 592-594.
Craig AM, Wyborski RJ, Banker G. Preferential addition of newly synthesized membrane protein at axonal growth cones. Nature. 1995;375(6532):592-594.
Craig, Ann Marie ; Wyborski, Russell J. ; Banker, Gary. / Preferential addition of newly synthesized membrane protein at axonal growth cones. In: Nature. 1995 ; Vol. 375, No. 6532. pp. 592-594.
@article{d260c4d05a2144bc86e197ace2a68e2b,
title = "Preferential addition of newly synthesized membrane protein at axonal growth cones",
abstract = "THE addition of plasma membrane proteins to a growing axon could occur by preferential insertion at the tip (the growth cone), by uniform insertion along the axon, or by insertion at the cell body and bulk flow along the axon. To differentiate between these possibilities we used a defective herpesvirus vector to express an exogenous protein, the lymphocyte transmembrane protein CD8α, in cultured rat hippocampal neurons. The newly synthesized protein first appeared on the axonal surface almost exclusively at the growth cone. Preferential addition at the growth cone was also observed in minor processes (immature dendrites), but not in mature dendrites. Over several hours, CD8α reached a uniform distribution over the entire neuronal surface, presumably by diffusion within the membrane and possibly endocytic recycling. As well as providing materials for axonal growth, the selective addition of membrane vesicles at the growth cone may contribute to the polarized distribution of axonal surface molecules1.",
author = "Craig, {Ann Marie} and Wyborski, {Russell J.} and Gary Banker",
year = "1995",
language = "English (US)",
volume = "375",
pages = "592--594",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6532",

}

TY - JOUR

T1 - Preferential addition of newly synthesized membrane protein at axonal growth cones

AU - Craig, Ann Marie

AU - Wyborski, Russell J.

AU - Banker, Gary

PY - 1995

Y1 - 1995

N2 - THE addition of plasma membrane proteins to a growing axon could occur by preferential insertion at the tip (the growth cone), by uniform insertion along the axon, or by insertion at the cell body and bulk flow along the axon. To differentiate between these possibilities we used a defective herpesvirus vector to express an exogenous protein, the lymphocyte transmembrane protein CD8α, in cultured rat hippocampal neurons. The newly synthesized protein first appeared on the axonal surface almost exclusively at the growth cone. Preferential addition at the growth cone was also observed in minor processes (immature dendrites), but not in mature dendrites. Over several hours, CD8α reached a uniform distribution over the entire neuronal surface, presumably by diffusion within the membrane and possibly endocytic recycling. As well as providing materials for axonal growth, the selective addition of membrane vesicles at the growth cone may contribute to the polarized distribution of axonal surface molecules1.

AB - THE addition of plasma membrane proteins to a growing axon could occur by preferential insertion at the tip (the growth cone), by uniform insertion along the axon, or by insertion at the cell body and bulk flow along the axon. To differentiate between these possibilities we used a defective herpesvirus vector to express an exogenous protein, the lymphocyte transmembrane protein CD8α, in cultured rat hippocampal neurons. The newly synthesized protein first appeared on the axonal surface almost exclusively at the growth cone. Preferential addition at the growth cone was also observed in minor processes (immature dendrites), but not in mature dendrites. Over several hours, CD8α reached a uniform distribution over the entire neuronal surface, presumably by diffusion within the membrane and possibly endocytic recycling. As well as providing materials for axonal growth, the selective addition of membrane vesicles at the growth cone may contribute to the polarized distribution of axonal surface molecules1.

UR - http://www.scopus.com/inward/record.url?scp=0029048041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029048041&partnerID=8YFLogxK

M3 - Article

VL - 375

SP - 592

EP - 594

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6532

ER -