Potential misidentification of cyclooxygenase-2 by western blot analysis and prevention through the inclusion of appropriate controls

Yen Ting Liu, Adel Kardosh, Janine Cooc, Axel H. Schönthal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cyclooxygenase (COX)-2 plays an important role in the development of cancer and has been recognized as a potential therapeutic target. Because nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the activity of this enzyme, the potential efficacy of such drugs for purposes of cancer prevention or therapy is an area of intense research. Therefore, it is of critical importance to unequivocally determine the expression levels of COX-2 protein in tumor cells. In this regard, there are several conflicting reports in the literature where the same type of tumor cell lines were reported as COX-2 positive and as COX-2 negative. We found that during Western blot analysis of COX-2 positive and COX-2 negative cells, different antibodies to COX-2 protein are able to generate strong signals, which are false-positives and can be confused with COX-2. Thus, we believe that some of the conflicting reports on COX-2 expression in tumor cell lines could be the result of improper interpretation of the Western blot signals. Here, we present some of these pitfalls and suggest the inclusion of appropriate controls to unequivocally identify COX-2 protein levels.

Original languageEnglish (US)
Pages (from-to)329-335
Number of pages7
JournalMolecular Biotechnology
Volume34
Issue number3
DOIs
StatePublished - Nov 1 2006
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Western Blotting
Tumors
Cells
Tumor Cell Line
Proteins
Prostaglandin-Endoperoxide Synthases
Neoplasms
Antibodies
Pharmaceutical Preparations
Anti-Inflammatory Agents
Enzymes

Keywords

  • COX-2 detection
  • Cyclooxygenase-2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Potential misidentification of cyclooxygenase-2 by western blot analysis and prevention through the inclusion of appropriate controls. / Liu, Yen Ting; Kardosh, Adel; Cooc, Janine; Schönthal, Axel H.

In: Molecular Biotechnology, Vol. 34, No. 3, 01.11.2006, p. 329-335.

Research output: Contribution to journalArticle

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