Post-inhibitory rebound properties of dopaminergic cells of the ventral tegmental area

Kush Paul, Steven Johnson

Research output: Contribution to journalArticle

Abstract

We have investigated the post-inhibitory rebound (PIR) properties of dopamine cells in the ventral tegmental area of the rat midbrain. Using microelectrodes to record intracellularly from rat cells in the midbrain slice, we found that 54 % of all recorded DA cells exhibited rebound depolarization in the presence of TTX. We observed primarily two types of rebound responses distinguished by their maximum amplitude and inactivation time constant, which we have termed as the 'slowly inactivating PIR' and low-threshold spike (LTS). 35% of DA cells exhibited a 'slowly inactivating PIR' and 19% showed LTS. A subset of the latter exhibited repetitive LTS. The PIR was enhanced in high K+ medium and suppressed in low-calcium medium. Under voltage clamp conditions, inward rebound currents at the end of a hyperpolarizing voltage step were partially blocked by specific T-type channel blocker, Ni 2+. Repetitive LTSs were reversibly changed to single LTS by blocking the potassium SK current.

Original languageEnglish (US)
Pages (from-to)147-157
Number of pages11
JournalNeuroscience Research Communications
Volume33
Issue number3
DOIs
StatePublished - Nov 2003

Fingerprint

Ventral Tegmental Area
Mesencephalon
Microelectrodes
Dopamine
Potassium
Calcium

Keywords

  • Brain slice
  • Calcium channel
  • Dopamine
  • Midbrain
  • Post-inhibitory rebound
  • Ventral tegmental area

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Post-inhibitory rebound properties of dopaminergic cells of the ventral tegmental area. / Paul, Kush; Johnson, Steven.

In: Neuroscience Research Communications, Vol. 33, No. 3, 11.2003, p. 147-157.

Research output: Contribution to journalArticle

@article{591bf825b6e7458592ff75653497aff3,
title = "Post-inhibitory rebound properties of dopaminergic cells of the ventral tegmental area",
abstract = "We have investigated the post-inhibitory rebound (PIR) properties of dopamine cells in the ventral tegmental area of the rat midbrain. Using microelectrodes to record intracellularly from rat cells in the midbrain slice, we found that 54 {\%} of all recorded DA cells exhibited rebound depolarization in the presence of TTX. We observed primarily two types of rebound responses distinguished by their maximum amplitude and inactivation time constant, which we have termed as the 'slowly inactivating PIR' and low-threshold spike (LTS). 35{\%} of DA cells exhibited a 'slowly inactivating PIR' and 19{\%} showed LTS. A subset of the latter exhibited repetitive LTS. The PIR was enhanced in high K+ medium and suppressed in low-calcium medium. Under voltage clamp conditions, inward rebound currents at the end of a hyperpolarizing voltage step were partially blocked by specific T-type channel blocker, Ni 2+. Repetitive LTSs were reversibly changed to single LTS by blocking the potassium SK current.",
keywords = "Brain slice, Calcium channel, Dopamine, Midbrain, Post-inhibitory rebound, Ventral tegmental area",
author = "Kush Paul and Steven Johnson",
year = "2003",
month = "11",
doi = "10.1002/nrc.10091",
language = "English (US)",
volume = "33",
pages = "147--157",
journal = "Neuroscience Research Communications",
issn = "0893-6609",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Post-inhibitory rebound properties of dopaminergic cells of the ventral tegmental area

AU - Paul, Kush

AU - Johnson, Steven

PY - 2003/11

Y1 - 2003/11

N2 - We have investigated the post-inhibitory rebound (PIR) properties of dopamine cells in the ventral tegmental area of the rat midbrain. Using microelectrodes to record intracellularly from rat cells in the midbrain slice, we found that 54 % of all recorded DA cells exhibited rebound depolarization in the presence of TTX. We observed primarily two types of rebound responses distinguished by their maximum amplitude and inactivation time constant, which we have termed as the 'slowly inactivating PIR' and low-threshold spike (LTS). 35% of DA cells exhibited a 'slowly inactivating PIR' and 19% showed LTS. A subset of the latter exhibited repetitive LTS. The PIR was enhanced in high K+ medium and suppressed in low-calcium medium. Under voltage clamp conditions, inward rebound currents at the end of a hyperpolarizing voltage step were partially blocked by specific T-type channel blocker, Ni 2+. Repetitive LTSs were reversibly changed to single LTS by blocking the potassium SK current.

AB - We have investigated the post-inhibitory rebound (PIR) properties of dopamine cells in the ventral tegmental area of the rat midbrain. Using microelectrodes to record intracellularly from rat cells in the midbrain slice, we found that 54 % of all recorded DA cells exhibited rebound depolarization in the presence of TTX. We observed primarily two types of rebound responses distinguished by their maximum amplitude and inactivation time constant, which we have termed as the 'slowly inactivating PIR' and low-threshold spike (LTS). 35% of DA cells exhibited a 'slowly inactivating PIR' and 19% showed LTS. A subset of the latter exhibited repetitive LTS. The PIR was enhanced in high K+ medium and suppressed in low-calcium medium. Under voltage clamp conditions, inward rebound currents at the end of a hyperpolarizing voltage step were partially blocked by specific T-type channel blocker, Ni 2+. Repetitive LTSs were reversibly changed to single LTS by blocking the potassium SK current.

KW - Brain slice

KW - Calcium channel

KW - Dopamine

KW - Midbrain

KW - Post-inhibitory rebound

KW - Ventral tegmental area

UR - http://www.scopus.com/inward/record.url?scp=0346374726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346374726&partnerID=8YFLogxK

U2 - 10.1002/nrc.10091

DO - 10.1002/nrc.10091

M3 - Article

VL - 33

SP - 147

EP - 157

JO - Neuroscience Research Communications

JF - Neuroscience Research Communications

SN - 0893-6609

IS - 3

ER -