Positive autoregulation of the myogenic determination gene MyoD1

Mathew (Matt) Thayer, Stephen J. Tapscott, Robert L. Davis, Woodring E. Wright, Andrew B. Lassar, Harold Weintraub

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

Transfection of cDNA expression vectors encoding either MyoD1 or myogenin into 10T1 2 cells converts them to myogenic cells. We show that transfection of 10T1 2 cells with the MyoD1 cDNA activates expression of endogenous MyoD1 mRNA, indicating that MyoD1 is subject to positive autoregulation. This activation of endogenous MyoD1 mRNA was also observed in Swiss 3T6 cells, but not in several other fibroblast or adipoblast cell lines transfected with the MyoD1 cDNA. In addition, transfection of the MyoD1 cDNA leads to activation of myogenin expression, and transfection of the myogenin cDNA leads to activation of MyoD1 expression. Thus, MyoD1 and myogenin appear to function in a positive autoregulatory loop that could either: account for or contribute to the stability of myogenic commitment; or amplify the level of expression of both MyoD1 and myogenin above a critical threshold that is required for activation of the myogenic program.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalCell
Volume58
Issue number2
DOIs
StatePublished - Jul 28 1989
Externally publishedYes

Fingerprint

Myogenin
Homeostasis
Complementary DNA
Genes
Transfection
Chemical activation
Messenger RNA
Fibroblasts
Cells
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Thayer, M. M., Tapscott, S. J., Davis, R. L., Wright, W. E., Lassar, A. B., & Weintraub, H. (1989). Positive autoregulation of the myogenic determination gene MyoD1. Cell, 58(2), 241-248. https://doi.org/10.1016/0092-8674(89)90838-6

Positive autoregulation of the myogenic determination gene MyoD1. / Thayer, Mathew (Matt); Tapscott, Stephen J.; Davis, Robert L.; Wright, Woodring E.; Lassar, Andrew B.; Weintraub, Harold.

In: Cell, Vol. 58, No. 2, 28.07.1989, p. 241-248.

Research output: Contribution to journalArticle

Thayer, MM, Tapscott, SJ, Davis, RL, Wright, WE, Lassar, AB & Weintraub, H 1989, 'Positive autoregulation of the myogenic determination gene MyoD1', Cell, vol. 58, no. 2, pp. 241-248. https://doi.org/10.1016/0092-8674(89)90838-6
Thayer MM, Tapscott SJ, Davis RL, Wright WE, Lassar AB, Weintraub H. Positive autoregulation of the myogenic determination gene MyoD1. Cell. 1989 Jul 28;58(2):241-248. https://doi.org/10.1016/0092-8674(89)90838-6
Thayer, Mathew (Matt) ; Tapscott, Stephen J. ; Davis, Robert L. ; Wright, Woodring E. ; Lassar, Andrew B. ; Weintraub, Harold. / Positive autoregulation of the myogenic determination gene MyoD1. In: Cell. 1989 ; Vol. 58, No. 2. pp. 241-248.
@article{90f4e8d326f149aea75d0d024d39b44e,
title = "Positive autoregulation of the myogenic determination gene MyoD1",
abstract = "Transfection of cDNA expression vectors encoding either MyoD1 or myogenin into 10T1 2 cells converts them to myogenic cells. We show that transfection of 10T1 2 cells with the MyoD1 cDNA activates expression of endogenous MyoD1 mRNA, indicating that MyoD1 is subject to positive autoregulation. This activation of endogenous MyoD1 mRNA was also observed in Swiss 3T6 cells, but not in several other fibroblast or adipoblast cell lines transfected with the MyoD1 cDNA. In addition, transfection of the MyoD1 cDNA leads to activation of myogenin expression, and transfection of the myogenin cDNA leads to activation of MyoD1 expression. Thus, MyoD1 and myogenin appear to function in a positive autoregulatory loop that could either: account for or contribute to the stability of myogenic commitment; or amplify the level of expression of both MyoD1 and myogenin above a critical threshold that is required for activation of the myogenic program.",
author = "Thayer, {Mathew (Matt)} and Tapscott, {Stephen J.} and Davis, {Robert L.} and Wright, {Woodring E.} and Lassar, {Andrew B.} and Harold Weintraub",
year = "1989",
month = "7",
day = "28",
doi = "10.1016/0092-8674(89)90838-6",
language = "English (US)",
volume = "58",
pages = "241--248",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Positive autoregulation of the myogenic determination gene MyoD1

AU - Thayer, Mathew (Matt)

AU - Tapscott, Stephen J.

AU - Davis, Robert L.

AU - Wright, Woodring E.

AU - Lassar, Andrew B.

AU - Weintraub, Harold

PY - 1989/7/28

Y1 - 1989/7/28

N2 - Transfection of cDNA expression vectors encoding either MyoD1 or myogenin into 10T1 2 cells converts them to myogenic cells. We show that transfection of 10T1 2 cells with the MyoD1 cDNA activates expression of endogenous MyoD1 mRNA, indicating that MyoD1 is subject to positive autoregulation. This activation of endogenous MyoD1 mRNA was also observed in Swiss 3T6 cells, but not in several other fibroblast or adipoblast cell lines transfected with the MyoD1 cDNA. In addition, transfection of the MyoD1 cDNA leads to activation of myogenin expression, and transfection of the myogenin cDNA leads to activation of MyoD1 expression. Thus, MyoD1 and myogenin appear to function in a positive autoregulatory loop that could either: account for or contribute to the stability of myogenic commitment; or amplify the level of expression of both MyoD1 and myogenin above a critical threshold that is required for activation of the myogenic program.

AB - Transfection of cDNA expression vectors encoding either MyoD1 or myogenin into 10T1 2 cells converts them to myogenic cells. We show that transfection of 10T1 2 cells with the MyoD1 cDNA activates expression of endogenous MyoD1 mRNA, indicating that MyoD1 is subject to positive autoregulation. This activation of endogenous MyoD1 mRNA was also observed in Swiss 3T6 cells, but not in several other fibroblast or adipoblast cell lines transfected with the MyoD1 cDNA. In addition, transfection of the MyoD1 cDNA leads to activation of myogenin expression, and transfection of the myogenin cDNA leads to activation of MyoD1 expression. Thus, MyoD1 and myogenin appear to function in a positive autoregulatory loop that could either: account for or contribute to the stability of myogenic commitment; or amplify the level of expression of both MyoD1 and myogenin above a critical threshold that is required for activation of the myogenic program.

UR - http://www.scopus.com/inward/record.url?scp=0024406874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024406874&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(89)90838-6

DO - 10.1016/0092-8674(89)90838-6

M3 - Article

VL - 58

SP - 241

EP - 248

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -