Platelet shape change and spreading

Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, Owen J.T. McCarty

Research output: Chapter in Book/Report/Conference proceedingChapter

36 Scopus citations

Abstract

Hemostasis is dependent upon the successful recruitment and activation of blood platelets to the site of a breach in the vasculature. Platelet activation stimulates the rapid reorganization of the cortical actin cytoskeleton, resulting in the transformation of platelets from biconcave disks to fully spread cells. During this process, platelets extend filopodia and generate lamellipodia, resulting in a dramatic increase in the platelet surface area. Kohler-illuminated Nomarski Differential Interference Contrast microscopy has proved an effective tool to characterize platelet morphological changes in real time, and provides a useful tool to identify genetic and pharmacological regulators of platelet function.

Original languageEnglish (US)
Title of host publicationPlatelets and Megakaryocytes
Subtitle of host publicationVolume 3, Additional Protocols and Perspectives
EditorsJonathan Gibbins, Martyn Mahaut-Smith
Pages91-100
Number of pages10
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume788
ISSN (Print)1064-3745

Keywords

  • Filopodia
  • Kohler-illuminated Nomarski DIC microscopy
  • Lamellipodia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Aslan, J. E., Itakura, A., Gertz, J. M., & McCarty, O. J. T. (2012). Platelet shape change and spreading. In J. Gibbins, & M. Mahaut-Smith (Eds.), Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives (pp. 91-100). (Methods in Molecular Biology; Vol. 788). https://doi.org/10.1007/978-1-61779-307-3_7