Platelet shape change and spreading

Joseph Aslan, Asako Itakura, Jacqueline M. Gertz, Owen McCarty

Research output: Chapter in Book/Report/Conference proceedingChapter

31 Citations (Scopus)

Abstract

Hemostasis is dependent upon the successful recruitment and activation of blood platelets to the site of a breach in the vasculature. Platelet activation stimulates the rapid reorganization of the cortical actin cytoskeleton, resulting in the transformation of platelets from biconcave disks to fully spread cells. During this process, platelets extend filopodia and generate lamellipodia, resulting in a dramatic increase in the platelet surface area. Kohler-illuminated Nomarski Differential Interference Contrast microscopy has proved an effective tool to characterize platelet morphological changes in real time, and provides a useful tool to identify genetic and pharmacological regulators of platelet function.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages91-100
Number of pages10
Volume788
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume788
ISSN (Print)10643745

Fingerprint

Blood Platelets
Interference Microscopy
Pseudopodia
Platelet Activation
Hemostasis
Actin Cytoskeleton
Pharmacology

Keywords

  • Filopodia
  • Kohler-illuminated Nomarski DIC microscopy
  • Lamellipodia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Aslan, J., Itakura, A., Gertz, J. M., & McCarty, O. (2012). Platelet shape change and spreading. In Methods in Molecular Biology (Vol. 788, pp. 91-100). (Methods in Molecular Biology; Vol. 788). https://doi.org/10.1007/978-1-61779-307-3_7

Platelet shape change and spreading. / Aslan, Joseph; Itakura, Asako; Gertz, Jacqueline M.; McCarty, Owen.

Methods in Molecular Biology. Vol. 788 2012. p. 91-100 (Methods in Molecular Biology; Vol. 788).

Research output: Chapter in Book/Report/Conference proceedingChapter

Aslan, J, Itakura, A, Gertz, JM & McCarty, O 2012, Platelet shape change and spreading. in Methods in Molecular Biology. vol. 788, Methods in Molecular Biology, vol. 788, pp. 91-100. https://doi.org/10.1007/978-1-61779-307-3_7
Aslan J, Itakura A, Gertz JM, McCarty O. Platelet shape change and spreading. In Methods in Molecular Biology. Vol. 788. 2012. p. 91-100. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-307-3_7
Aslan, Joseph ; Itakura, Asako ; Gertz, Jacqueline M. ; McCarty, Owen. / Platelet shape change and spreading. Methods in Molecular Biology. Vol. 788 2012. pp. 91-100 (Methods in Molecular Biology).
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