Plasmodium falciparum: Cytoadherence of malaria-infected erythrocytes to human brain capillary and umbilical vein endothelial cells-A comparative study of adhesive ligands

Heidi Smith, Jay Nelson, Carl G. Gahmberg, Ian Crandall, Irwin W. Sherman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalExperimental Parasitology
Volume75
Issue number3
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Umbilical Veins
Falciparum Malaria
Amelanotic Melanoma
Adhesives
Endothelial Cells
Erythrocytes
Ligands
Human Umbilical Vein Endothelial Cells
Brain
Plasmodium falciparum
Adhesiveness
Intercellular Adhesion Molecule-1
Indirect Fluorescent Antibody Technique
Glycoproteins
Monoclonal Antibodies
In Vitro Techniques

Keywords

  • Amelanotic melanoma cells
  • Brain endothelial cells
  • CD36
  • Cytoadherence
  • ICAM-1
  • ICAM-2
  • Malaria
  • Plasmodium falciparum
  • Umbilical vein endothelial cells

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Plasmodium falciparum : Cytoadherence of malaria-infected erythrocytes to human brain capillary and umbilical vein endothelial cells-A comparative study of adhesive ligands. / Smith, Heidi; Nelson, Jay; Gahmberg, Carl G.; Crandall, Ian; Sherman, Irwin W.

In: Experimental Parasitology, Vol. 75, No. 3, 1992, p. 269-280.

Research output: Contribution to journalArticle

@article{70891b4f6fb34b46b2038776892c6e71,
title = "Plasmodium falciparum: Cytoadherence of malaria-infected erythrocytes to human brain capillary and umbilical vein endothelial cells-A comparative study of adhesive ligands",
abstract = "The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.",
keywords = "Amelanotic melanoma cells, Brain endothelial cells, CD36, Cytoadherence, ICAM-1, ICAM-2, Malaria, Plasmodium falciparum, Umbilical vein endothelial cells",
author = "Heidi Smith and Jay Nelson and Gahmberg, {Carl G.} and Ian Crandall and Sherman, {Irwin W.}",
year = "1992",
doi = "10.1016/0014-4894(92)90212-S",
language = "English (US)",
volume = "75",
pages = "269--280",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Plasmodium falciparum

T2 - Cytoadherence of malaria-infected erythrocytes to human brain capillary and umbilical vein endothelial cells-A comparative study of adhesive ligands

AU - Smith, Heidi

AU - Nelson, Jay

AU - Gahmberg, Carl G.

AU - Crandall, Ian

AU - Sherman, Irwin W.

PY - 1992

Y1 - 1992

N2 - The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.

AB - The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.

KW - Amelanotic melanoma cells

KW - Brain endothelial cells

KW - CD36

KW - Cytoadherence

KW - ICAM-1

KW - ICAM-2

KW - Malaria

KW - Plasmodium falciparum

KW - Umbilical vein endothelial cells

UR - http://www.scopus.com/inward/record.url?scp=0026954372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026954372&partnerID=8YFLogxK

U2 - 10.1016/0014-4894(92)90212-S

DO - 10.1016/0014-4894(92)90212-S

M3 - Article

C2 - 1385207

AN - SCOPUS:0026954372

VL - 75

SP - 269

EP - 280

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3

ER -