TY - JOUR
T1 - Plasmodium falciparum
T2 - Cytoadherence of malaria-infected erythrocytes to human brain capillary and umbilical vein endothelial cells-A comparative study of adhesive ligands
AU - Smith, Heidi
AU - Nelson, Jay A.
AU - Gahmberg, Carl G.
AU - Crandall, Ian
AU - Sherman, Irwin W.
PY - 1992/11
Y1 - 1992/11
N2 - The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.
AB - The cytoadherence of Plasmodium falciparum-infected erythrocytes (FCR-3 line) to human brain capillary endothelial cells (HBEC), C32 amelanotic melanoma cells, and human umbilical vein endothelial cells (HUVEC) was studied. The adhesion of infected red cells was HBEC > amelanotic melanoma > HUVEC. The presence or absence of the adhesive ligands ICAM-1 (CD54 or intercellular adhesion molecule 1), ICAM-2, and CD36 (=glycoprotein IV) was determined for each of these cells by indirect immunofluorescence using the monoclonal antibodies RR1/1, 6D5, and OKM 5/OKM 8, respectively. It appeared that a major ligand for the FCR-3 line of P. falciparum with amelanotic melanoma cells and HBECs was CD36. Binding to HUVECs was very low, presumably due to their lack of expression of CD36. HBECs, because of their ease of in vitro propagation, long-term maintenance of cytoadherent properties, and their high degree of adhesiveness, will be useful for in vitro studies of adherence.
KW - Amelanotic melanoma cells
KW - Brain endothelial cells
KW - CD36
KW - Cytoadherence
KW - ICAM-1
KW - ICAM-2
KW - Malaria
KW - Plasmodium falciparum
KW - Umbilical vein endothelial cells
UR - http://www.scopus.com/inward/record.url?scp=0026954372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026954372&partnerID=8YFLogxK
U2 - 10.1016/0014-4894(92)90212-S
DO - 10.1016/0014-4894(92)90212-S
M3 - Article
C2 - 1385207
AN - SCOPUS:0026954372
SN - 0014-4894
VL - 75
SP - 269
EP - 280
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -