Placental peroxisome proliferator-activated receptor-γ is up-regulated by pregnancy serum

Leslie L. Waite, Eric C. Person, Yan Zhou, Kee Hak Lim, Thomas (Tom) Scanlan, Robert N. Taylor

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Lipid metabolism plays an important role in normal pregnancy adaptation and in pathological pregnancy (e.g. preeclampsia). In the current studies we examined the expression of peroxisome proliferator-activated receptor-γ (PPARγ) in tissues and cells relevant to human pregnancy. We found that PPARγ is expressed in placental cytotrophoblasts in vivo and in trophoblasts (primary and choriocarcinoma cells) and fetal endothelial cells in vitro. We characterized primary cytotrophoblasts and two cell lines with which to study PPARγ regulation in human pregnancy. Like primary cytotrophoblasts, the choriocarcinoma cell line JEG-3 has endogenous PPARγ expression. Normal positive and negative PPARγ regulation was observed in the latter cells. We also created a new JEG-3-derived cell line (EP-JEG) by stable insertion of a PPAR response element-luciferase reporter gene construct. Together, these cell lines are useful for studying PPARγ expression and activation in human trophoblasts. We examined PPARγ regulation in these cells by human serum and found that PPARγ protein expression and activation are dramatically increased by sera from pregnant women. Preliminary characterization of the regulatory principle(s) is consistent with a prostanoid or fatty acid derivative. The results suggest that increased activation of PPARγ may play an important role in maternal metabolism during human pregnancy.

Original languageEnglish (US)
Pages (from-to)3808-3814
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume85
Issue number10
StatePublished - 2000
Externally publishedYes

Fingerprint

Peroxisome Proliferator-Activated Receptors
Pregnancy
Trophoblasts
Serum
Cells
Cell Line
Choriocarcinoma
Chemical activation
Endothelial cells
Response Elements
Pre-Eclampsia
Luciferases
Reporter Genes
Lipid Metabolism
Metabolism
Prostaglandins
Pregnant Women
Fatty Acids
Endothelial Cells
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Placental peroxisome proliferator-activated receptor-γ is up-regulated by pregnancy serum. / Waite, Leslie L.; Person, Eric C.; Zhou, Yan; Lim, Kee Hak; Scanlan, Thomas (Tom); Taylor, Robert N.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 85, No. 10, 2000, p. 3808-3814.

Research output: Contribution to journalArticle

Waite, Leslie L. ; Person, Eric C. ; Zhou, Yan ; Lim, Kee Hak ; Scanlan, Thomas (Tom) ; Taylor, Robert N. / Placental peroxisome proliferator-activated receptor-γ is up-regulated by pregnancy serum. In: Journal of Clinical Endocrinology and Metabolism. 2000 ; Vol. 85, No. 10. pp. 3808-3814.
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