Physiological levels of blood coagulation factors IX and X control coagulation kinetics in an in vitro model of circulating tissue factor

Garth W. Tormoen, Ayesha Khader, Andras Gruber, Owen McCarty

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Thrombosis significantly contributes to cancer morbidity and mortality. The mechanism behind thrombosis in cancer may be circulating tissue factor (TF), as levels of circulating TF are associated with thrombosis. However, circulating TF antigen level alone has failed to predict thrombosis in patients with cancer. We hypothesize that coagulation factor levels regulate the kinetics of circulating TF-induced thrombosis. Coagulation kinetics were measured as a function of individual coagulation factor levels and TF particle concentration. Clotting times increased when pooled plasma was mixed at or above a ratio of 4:6 with PBS. Clotting times increased when pooled plasma was mixed at or above a ratio of 8:2 with factor VII-depleted plasma, 7:3 with factor IX- or factor X-depleted plasmas, or 2:8 with factor II-, V- or VIII-depleted plasmas. Addition of coagulation factors VII, X, IX, V and II to depleted plasmas shortened clotting and enzyme initiation times, and increased enzyme generation rates in a concentration-dependent manner. Only additions of factors IX and X from low-normal to high-normal levels shortened clotting times and increased enzyme generation rates. Our results demonstrate that coagulation kinetics for TF particles are controlled by factor IX and X levels within the normal physiological range. We hypothesize that individual patient factor IX and X levels may be prognostic for susceptibility to circulating TF-induced thrombosis.

Original languageEnglish (US)
Article number036003
JournalPhysical Biology
Volume10
Issue number3
DOIs
StatePublished - Jun 2013

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Factor X
Factor IX
Thromboplastin
Thrombosis
Factor VII
Blood Coagulation Factors
Neoplasms
Factor VIII
Prothrombin
Enzymes
In Vitro Techniques
Reference Values
Morbidity
Antigens
Mortality

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Structural Biology
  • Medicine(all)

Cite this

Physiological levels of blood coagulation factors IX and X control coagulation kinetics in an in vitro model of circulating tissue factor. / Tormoen, Garth W.; Khader, Ayesha; Gruber, Andras; McCarty, Owen.

In: Physical Biology, Vol. 10, No. 3, 036003, 06.2013.

Research output: Contribution to journalArticle

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