Physicochemical characterization of poly(l-lactic acid) and poly(d,l-lactide-co-glycolide) nanoparticles with polyethylenimine as gene delivery carrier

In Sook Kim, Soo Kyung Lee, Yu Mi Park, Yong Bok Lee, Sang Chul Shin, Kang Choon Lee, In Joon Oh

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Polymer nanoparticles have been used as non-viral gene delivery systems and drug delivery systems. In this study, biodegradable poly(l-lactic acid) (PLA)/polyethylenimine (PEI) and poly(d,l-lactide-co-glycolide) (PLGA)/PEI nanoparticles were prepared and characterized as gene delivery systems. The PLA/PEI and PLGA/PEI nanoparticles, which were prepared by a diafiltration method, had spherical shapes and smooth surface characteristics. The size of nanoparticles was controlled by the amount of PEI, which acted as a hydrophilic moiety, which effectively reduced the interfacial energy between the particle surface and the aqueous media. The nanoparticles showed an excellent dispersive stability under storage in a phosphate-buffered saline solution for 12 days. The positive zeta-potentials for the nanoparticles decreased and changed to negative values with increasing plasmid DNA (pDNA) content. Agarose gel electrophoresis showed that the complex formation between the nanoparticles and the pDNA coincided with the zeta-potential results. The results of in vitro transfection and cell viability on HEK 293 cells indicated that the nanoparticles could be used as gene delivery carriers.

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume298
Issue number1
DOIs
StatePublished - Jul 14 2005

Keywords

  • Gene delivery
  • Nanoparticles
  • Poly(d,l-lactide-co-glycolide)
  • Poly(l-lactic acid)
  • Polyethylenimine

ASJC Scopus subject areas

  • Pharmaceutical Science

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