Abstract
The genetic locus for incomplete congenital stationary night blindness (CSNB2) has been identified as the CACNA1f gene, encoding the α1F calcium channel subunit, a member of the L-type family of calcium channels. The electroretinogram associated with CSNB2 implicates α1F in synaptic transmission between retinal photoreceptors and bipolar cells. Using a recently developed monoclonal antibody to α1F, we localize the channel to ribbon active zones in rod photoreceptor terminals of the mouse retina, supporting a role for α1F in mediating glutamate release from rods. Detergent extraction experiments indicate that α1F is part of a detergent-resistant active zone complex, which also includes the synaptic ribbons. Comparison of native mouse rod calcium currents with recombinant α1F currents reveals that the current-voltage relationship for the native current is shifted approximately 30 mV to more hyperpolarized potentials than for the recombinant α1F current, suggesting modulation of the native channel by intracellular factors. Lastly, we present evidence for L-type α1D calcium channel subunits in cone terminals of the mouse retina. The presence of α1D channels in cones may explain the residual visual abilities of individuals with CSNB2.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 561-568 |
| Number of pages | 8 |
| Journal | Visual Neuroscience |
| Volume | 22 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 2005 |
Fingerprint
Keywords
- Calcium channel
- CSNB2
- Photoreceptor
- Ribbon synapse
- Synaptic transmission
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Photoreceptor calcium channels : Insight from night blindness. / Morgans, Catherine; Bayley, Philippa R.; Oesch, Nicholas W.; Ren, Gaoying; Akileswaran, Lakshmi; Taylor, William.
In: Visual Neuroscience, Vol. 22, No. 5, 09.2005, p. 561-568.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photoreceptor calcium channels
T2 - Insight from night blindness
AU - Morgans, Catherine
AU - Bayley, Philippa R.
AU - Oesch, Nicholas W.
AU - Ren, Gaoying
AU - Akileswaran, Lakshmi
AU - Taylor, William
PY - 2005/9
Y1 - 2005/9
N2 - The genetic locus for incomplete congenital stationary night blindness (CSNB2) has been identified as the CACNA1f gene, encoding the α1F calcium channel subunit, a member of the L-type family of calcium channels. The electroretinogram associated with CSNB2 implicates α1F in synaptic transmission between retinal photoreceptors and bipolar cells. Using a recently developed monoclonal antibody to α1F, we localize the channel to ribbon active zones in rod photoreceptor terminals of the mouse retina, supporting a role for α1F in mediating glutamate release from rods. Detergent extraction experiments indicate that α1F is part of a detergent-resistant active zone complex, which also includes the synaptic ribbons. Comparison of native mouse rod calcium currents with recombinant α1F currents reveals that the current-voltage relationship for the native current is shifted approximately 30 mV to more hyperpolarized potentials than for the recombinant α1F current, suggesting modulation of the native channel by intracellular factors. Lastly, we present evidence for L-type α1D calcium channel subunits in cone terminals of the mouse retina. The presence of α1D channels in cones may explain the residual visual abilities of individuals with CSNB2.
AB - The genetic locus for incomplete congenital stationary night blindness (CSNB2) has been identified as the CACNA1f gene, encoding the α1F calcium channel subunit, a member of the L-type family of calcium channels. The electroretinogram associated with CSNB2 implicates α1F in synaptic transmission between retinal photoreceptors and bipolar cells. Using a recently developed monoclonal antibody to α1F, we localize the channel to ribbon active zones in rod photoreceptor terminals of the mouse retina, supporting a role for α1F in mediating glutamate release from rods. Detergent extraction experiments indicate that α1F is part of a detergent-resistant active zone complex, which also includes the synaptic ribbons. Comparison of native mouse rod calcium currents with recombinant α1F currents reveals that the current-voltage relationship for the native current is shifted approximately 30 mV to more hyperpolarized potentials than for the recombinant α1F current, suggesting modulation of the native channel by intracellular factors. Lastly, we present evidence for L-type α1D calcium channel subunits in cone terminals of the mouse retina. The presence of α1D channels in cones may explain the residual visual abilities of individuals with CSNB2.
KW - Calcium channel
KW - CSNB2
KW - Photoreceptor
KW - Ribbon synapse
KW - Synaptic transmission
UR - http://www.scopus.com/inward/record.url?scp=32544444462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32544444462&partnerID=8YFLogxK
U2 - 10.1017/S0952523805225038
DO - 10.1017/S0952523805225038
M3 - Article
C2 - 16332266
AN - SCOPUS:32544444462
VL - 22
SP - 561
EP - 568
JO - Visual Neuroscience
JF - Visual Neuroscience
SN - 0952-5238
IS - 5
ER -