Photoreceptor calcium channels: Insight from night blindness

Catherine Morgans, Philippa R. Bayley, Nicholas W. Oesch, Gaoying Ren, Lakshmi Akileswaran, William Taylor

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The genetic locus for incomplete congenital stationary night blindness (CSNB2) has been identified as the CACNA1f gene, encoding the α1F calcium channel subunit, a member of the L-type family of calcium channels. The electroretinogram associated with CSNB2 implicates α1F in synaptic transmission between retinal photoreceptors and bipolar cells. Using a recently developed monoclonal antibody to α1F, we localize the channel to ribbon active zones in rod photoreceptor terminals of the mouse retina, supporting a role for α1F in mediating glutamate release from rods. Detergent extraction experiments indicate that α1F is part of a detergent-resistant active zone complex, which also includes the synaptic ribbons. Comparison of native mouse rod calcium currents with recombinant α1F currents reveals that the current-voltage relationship for the native current is shifted approximately 30 mV to more hyperpolarized potentials than for the recombinant α1F current, suggesting modulation of the native channel by intracellular factors. Lastly, we present evidence for L-type α1D calcium channel subunits in cone terminals of the mouse retina. The presence of α1D channels in cones may explain the residual visual abilities of individuals with CSNB2.

Original languageEnglish (US)
Pages (from-to)561-568
Number of pages8
JournalVisual Neuroscience
Volume22
Issue number5
DOIs
StatePublished - Sep 2005

Fingerprint

Night Blindness
Calcium Channels
L-Type Calcium Channels
Detergents
Retina
Retinal Bipolar Cells
Retinal Rod Photoreceptor Cells
Vertebrate Photoreceptor Cells
Aptitude
Genetic Loci
Synaptic Transmission
Glutamic Acid
Monoclonal Antibodies
Calcium
Congenital stationary night blindness
Genes

Keywords

  • Calcium channel
  • CSNB2
  • Photoreceptor
  • Ribbon synapse
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Morgans, C., Bayley, P. R., Oesch, N. W., Ren, G., Akileswaran, L., & Taylor, W. (2005). Photoreceptor calcium channels: Insight from night blindness. Visual Neuroscience, 22(5), 561-568. https://doi.org/10.1017/S0952523805225038

Photoreceptor calcium channels : Insight from night blindness. / Morgans, Catherine; Bayley, Philippa R.; Oesch, Nicholas W.; Ren, Gaoying; Akileswaran, Lakshmi; Taylor, William.

In: Visual Neuroscience, Vol. 22, No. 5, 09.2005, p. 561-568.

Research output: Contribution to journalArticle

Morgans, C, Bayley, PR, Oesch, NW, Ren, G, Akileswaran, L & Taylor, W 2005, 'Photoreceptor calcium channels: Insight from night blindness', Visual Neuroscience, vol. 22, no. 5, pp. 561-568. https://doi.org/10.1017/S0952523805225038
Morgans, Catherine ; Bayley, Philippa R. ; Oesch, Nicholas W. ; Ren, Gaoying ; Akileswaran, Lakshmi ; Taylor, William. / Photoreceptor calcium channels : Insight from night blindness. In: Visual Neuroscience. 2005 ; Vol. 22, No. 5. pp. 561-568.
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