Phospholipase C-γ1 association with CD3 structure in T cells

Jai Dev Dasgupta, Clarissa Granja, Brian Druker, Lih Ling Lin, Edmond J. Yunis, Valerie Relias

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Recently, we and others have reported tyrosine phosphorylation of phospholipase C-γ1 (PUCγ1) enzyme after CD3 activation of T cells, and have proposed that PLCγ1 mediates signal transduction through the T cell receptor (TCR/CD3). Here, using immunoblotting and immune complex PLC assays, we show that CD3 stimulation of Jurkat cells induces the association of PLCγ1 enzyme with CD3 complex. PLC activity is also found to co-precipitate with the CD3ζ chain from activated cells. In addition, in vitro PLC assays show that CD3 activation leads to about 10-fold stimulation of PLCγ1 activity. These results, along with the observation that Jurkat cells preferentially express PLCγ1, indicate that PLCγ1 participates in CD3 signaling.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalJournal of Experimental Medicine
Volume175
Issue number1
StatePublished - 1992
Externally publishedYes

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Jurkat Cells
Type C Phospholipases
CD3 Antigens
T-Lymphocytes
Enzymes
T-Cell Antigen Receptor
Antigen-Antibody Complex
Immunoblotting
Tyrosine
Signal Transduction
Phosphorylation
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Dasgupta, J. D., Granja, C., Druker, B., Lin, L. L., Yunis, E. J., & Relias, V. (1992). Phospholipase C-γ1 association with CD3 structure in T cells. Journal of Experimental Medicine, 175(1), 285-288.

Phospholipase C-γ1 association with CD3 structure in T cells. / Dasgupta, Jai Dev; Granja, Clarissa; Druker, Brian; Lin, Lih Ling; Yunis, Edmond J.; Relias, Valerie.

In: Journal of Experimental Medicine, Vol. 175, No. 1, 1992, p. 285-288.

Research output: Contribution to journalArticle

Dasgupta, JD, Granja, C, Druker, B, Lin, LL, Yunis, EJ & Relias, V 1992, 'Phospholipase C-γ1 association with CD3 structure in T cells', Journal of Experimental Medicine, vol. 175, no. 1, pp. 285-288.
Dasgupta JD, Granja C, Druker B, Lin LL, Yunis EJ, Relias V. Phospholipase C-γ1 association with CD3 structure in T cells. Journal of Experimental Medicine. 1992;175(1):285-288.
Dasgupta, Jai Dev ; Granja, Clarissa ; Druker, Brian ; Lin, Lih Ling ; Yunis, Edmond J. ; Relias, Valerie. / Phospholipase C-γ1 association with CD3 structure in T cells. In: Journal of Experimental Medicine. 1992 ; Vol. 175, No. 1. pp. 285-288.
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