Phospholipase C-γ1 association with CD3 structure in T cells

Jai Dev Dasgupta, Clarissa Granja, Brian Druker, Lih Ling Lin, Edmond J. Yunis, Valerie Relias

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Recently, we and others have reported tyrosine phosphorylation of phospholipase C-γ1 (PLCγ,1)enzyme after CD3 activation of T cells, and have proposed that PLCγl mediates signal transduction through the T cell receptor (TCR/CD3). Here, using immunoblotting and immune complex PLC assays, we show that CD3 stimulation of Jurkat cells induces the association of PLCγ1 enzymewith CD3 complex. PLC activity is also found to co-precipitate with the CD3ζ chain from activated cells. In addition, in vitro PLC assays show that CD3 activation leads to about10-fold stimulation of PLCγ1 activity. Theseresults, along with the observation that Jurkat cells preferentially express PLCγ1, indicate that PLCγ1 participates in CD3 signaling.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalJournal of Experimental Medicine
Issue number1
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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