Perturbation Biology

Inferring Signaling Networks in Cellular Systems

Evan J. Molinelli, Anil Korkut, Weiqing Wang, Martin L. Miller, Nicholas P. Gauthier, Xiaohong Jing, Poorvi Kaushik, Qin He, Gordon Mills, David B. Solit, Christine A. Pratilas, Martin Weigt, Alfredo Braunstein, Andrea Pagnani, Riccardo Zecchina, Chris Sander

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We present a powerful experimental-computational technology for inferring network models that predict the response of cells to perturbations, and that may be useful in the design of combinatorial therapy against cancer. The experiments are systematic series of perturbations of cancer cell lines by targeted drugs, singly or in combination. The response to perturbation is quantified in terms of relative changes in the measured levels of proteins, phospho-proteins and cellular phenotypes such as viability. Computational network models are derived de novo, i.e., without prior knowledge of signaling pathways, and are based on simple non-linear differential equations. The prohibitively large solution space of all possible network models is explored efficiently using a probabilistic algorithm, Belief Propagation (BP), which is three orders of magnitude faster than standard Monte Carlo methods. Explicit executable models are derived for a set of perturbation experiments in SKMEL-133 melanoma cell lines, which are resistant to the therapeutically important inhibitor of RAF kinase. The resulting network models reproduce and extend known pathway biology. They empower potential discoveries of new molecular interactions and predict efficacious novel drug perturbations, such as the inhibition of PLK1, which is verified experimentally. This technology is suitable for application to larger systems in diverse areas of molecular biology.

Original languageEnglish (US)
Article numbere1003290
JournalPLoS Computational Biology
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

Fingerprint

Cellular Systems
Biology
perturbation
Network Model
Technology
Perturbation
Monte Carlo Method
Cell Line
Biological Sciences
Pharmaceutical Preparations
Molecular Biology
Melanoma
Neoplasms
Proteins
Phosphotransferases
cancer
Cancer
Cell
Phenotype
Drugs

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Molinelli, E. J., Korkut, A., Wang, W., Miller, M. L., Gauthier, N. P., Jing, X., ... Sander, C. (2013). Perturbation Biology: Inferring Signaling Networks in Cellular Systems. PLoS Computational Biology, 9(12), [e1003290]. https://doi.org/10.1371/journal.pcbi.1003290

Perturbation Biology : Inferring Signaling Networks in Cellular Systems. / Molinelli, Evan J.; Korkut, Anil; Wang, Weiqing; Miller, Martin L.; Gauthier, Nicholas P.; Jing, Xiaohong; Kaushik, Poorvi; He, Qin; Mills, Gordon; Solit, David B.; Pratilas, Christine A.; Weigt, Martin; Braunstein, Alfredo; Pagnani, Andrea; Zecchina, Riccardo; Sander, Chris.

In: PLoS Computational Biology, Vol. 9, No. 12, e1003290, 01.12.2013.

Research output: Contribution to journalArticle

Molinelli, EJ, Korkut, A, Wang, W, Miller, ML, Gauthier, NP, Jing, X, Kaushik, P, He, Q, Mills, G, Solit, DB, Pratilas, CA, Weigt, M, Braunstein, A, Pagnani, A, Zecchina, R & Sander, C 2013, 'Perturbation Biology: Inferring Signaling Networks in Cellular Systems', PLoS Computational Biology, vol. 9, no. 12, e1003290. https://doi.org/10.1371/journal.pcbi.1003290
Molinelli EJ, Korkut A, Wang W, Miller ML, Gauthier NP, Jing X et al. Perturbation Biology: Inferring Signaling Networks in Cellular Systems. PLoS Computational Biology. 2013 Dec 1;9(12). e1003290. https://doi.org/10.1371/journal.pcbi.1003290
Molinelli, Evan J. ; Korkut, Anil ; Wang, Weiqing ; Miller, Martin L. ; Gauthier, Nicholas P. ; Jing, Xiaohong ; Kaushik, Poorvi ; He, Qin ; Mills, Gordon ; Solit, David B. ; Pratilas, Christine A. ; Weigt, Martin ; Braunstein, Alfredo ; Pagnani, Andrea ; Zecchina, Riccardo ; Sander, Chris. / Perturbation Biology : Inferring Signaling Networks in Cellular Systems. In: PLoS Computational Biology. 2013 ; Vol. 9, No. 12.
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