Percentage of Philadelphia Chromosome (Ph)-Negative and Ph-Positive Cells Found after Autologous Transplantation for Chronic Myelogenous Leukemia Depends on Percentage of Diploid Cells Induced by Conventional-Dose Chemotherapy before Collection of Autologous Cells

Moshe Talpaz, Hagop Kantarjian, Jan Liang, Leslie Calvert, Joy Hamer, Patricia Tibbits, April Durett, David Claxton, Sergio Giralt, Issa Khouri, Donna Przepiorka, Koen Van Besien, Borje Andersson, Rakesh Mehra, James Gajewski, David Seong, Jeane Hester, Elihu Estey, Martin Korbling, Nicoletta PollicardoRonald Berenson, Shelly Heimfeld, Richard Champlin, Albert B. Deisseroth

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We collected peripheral blood mononuclear cells and bone marrow cells soon after recovery from conventional-dose chemotherapy-induced myelosuppression and transplanted these cells into advanced chronic myelogenous leukemia (CML) patients after treating these patients with 120 mg/kg cyclophosphamide, 750 mg/m2 VP-16, and 1,020 cGy of total body irradiation (TBI). Of the 10 late chronic-phase patients and the eight accelerated-phase CML patients evaluable posttransplant, 90% and 87%, respectively, remain alive posttransplant, whereas none of the three blast crisis CML patients given this therapy remain alive posttransplant. We measured the percentage of Philadelphia chromosome (Ph)-negative cells in the autologous cells collected after conventional-dose chemotherapy-induced myelosuppression before autologous transplant and in the marrow of these same CML patients after autologous transplantation of these cells into recipients treated with the cyclophosphamide, VP-16, and TBI. A direct correlation (correlation coefficient = 0.91) was observed between the level of Ph+ cells in the transplanted cells and the percentage of Ph+ marrow cells after transplant in 21 patients so transplanted. The data show that the chance of generating cytogenetic remissions posttransplant depends on the percentage of diploid cells in the preparations of autologous cells used for transplant and the stage of disease of the patients at the time of collection of the autologous cells.

Original languageEnglish (US)
Pages (from-to)3257-3263
Number of pages7
JournalBlood
Volume85
Issue number11
StatePublished - Jun 1 1995
Externally publishedYes

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Philadelphia Chromosome
Transplants
Chemotherapy
Autologous Transplantation
Etoposide
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Chromosomes
Diploidy
Cyclophosphamide
Cells
Irradiation
Viral Structural Proteins
Drug Therapy
Autografts
Bone
Blood
Recovery
Whole-Body Irradiation
Bone Marrow
Leukemia, Myeloid, Chronic Phase

ASJC Scopus subject areas

  • Hematology

Cite this

Percentage of Philadelphia Chromosome (Ph)-Negative and Ph-Positive Cells Found after Autologous Transplantation for Chronic Myelogenous Leukemia Depends on Percentage of Diploid Cells Induced by Conventional-Dose Chemotherapy before Collection of Autologous Cells. / Talpaz, Moshe; Kantarjian, Hagop; Liang, Jan; Calvert, Leslie; Hamer, Joy; Tibbits, Patricia; Durett, April; Claxton, David; Giralt, Sergio; Khouri, Issa; Przepiorka, Donna; Van Besien, Koen; Andersson, Borje; Mehra, Rakesh; Gajewski, James; Seong, David; Hester, Jeane; Estey, Elihu; Korbling, Martin; Pollicardo, Nicoletta; Berenson, Ronald; Heimfeld, Shelly; Champlin, Richard; Deisseroth, Albert B.

In: Blood, Vol. 85, No. 11, 01.06.1995, p. 3257-3263.

Research output: Contribution to journalArticle

Talpaz, M, Kantarjian, H, Liang, J, Calvert, L, Hamer, J, Tibbits, P, Durett, A, Claxton, D, Giralt, S, Khouri, I, Przepiorka, D, Van Besien, K, Andersson, B, Mehra, R, Gajewski, J, Seong, D, Hester, J, Estey, E, Korbling, M, Pollicardo, N, Berenson, R, Heimfeld, S, Champlin, R & Deisseroth, AB 1995, 'Percentage of Philadelphia Chromosome (Ph)-Negative and Ph-Positive Cells Found after Autologous Transplantation for Chronic Myelogenous Leukemia Depends on Percentage of Diploid Cells Induced by Conventional-Dose Chemotherapy before Collection of Autologous Cells', Blood, vol. 85, no. 11, pp. 3257-3263.
Talpaz, Moshe ; Kantarjian, Hagop ; Liang, Jan ; Calvert, Leslie ; Hamer, Joy ; Tibbits, Patricia ; Durett, April ; Claxton, David ; Giralt, Sergio ; Khouri, Issa ; Przepiorka, Donna ; Van Besien, Koen ; Andersson, Borje ; Mehra, Rakesh ; Gajewski, James ; Seong, David ; Hester, Jeane ; Estey, Elihu ; Korbling, Martin ; Pollicardo, Nicoletta ; Berenson, Ronald ; Heimfeld, Shelly ; Champlin, Richard ; Deisseroth, Albert B. / Percentage of Philadelphia Chromosome (Ph)-Negative and Ph-Positive Cells Found after Autologous Transplantation for Chronic Myelogenous Leukemia Depends on Percentage of Diploid Cells Induced by Conventional-Dose Chemotherapy before Collection of Autologous Cells. In: Blood. 1995 ; Vol. 85, No. 11. pp. 3257-3263.
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abstract = "We collected peripheral blood mononuclear cells and bone marrow cells soon after recovery from conventional-dose chemotherapy-induced myelosuppression and transplanted these cells into advanced chronic myelogenous leukemia (CML) patients after treating these patients with 120 mg/kg cyclophosphamide, 750 mg/m2 VP-16, and 1,020 cGy of total body irradiation (TBI). Of the 10 late chronic-phase patients and the eight accelerated-phase CML patients evaluable posttransplant, 90{\%} and 87{\%}, respectively, remain alive posttransplant, whereas none of the three blast crisis CML patients given this therapy remain alive posttransplant. We measured the percentage of Philadelphia chromosome (Ph)-negative cells in the autologous cells collected after conventional-dose chemotherapy-induced myelosuppression before autologous transplant and in the marrow of these same CML patients after autologous transplantation of these cells into recipients treated with the cyclophosphamide, VP-16, and TBI. A direct correlation (correlation coefficient = 0.91) was observed between the level of Ph+ cells in the transplanted cells and the percentage of Ph+ marrow cells after transplant in 21 patients so transplanted. The data show that the chance of generating cytogenetic remissions posttransplant depends on the percentage of diploid cells in the preparations of autologous cells used for transplant and the stage of disease of the patients at the time of collection of the autologous cells.",
author = "Moshe Talpaz and Hagop Kantarjian and Jan Liang and Leslie Calvert and Joy Hamer and Patricia Tibbits and April Durett and David Claxton and Sergio Giralt and Issa Khouri and Donna Przepiorka and {Van Besien}, Koen and Borje Andersson and Rakesh Mehra and James Gajewski and David Seong and Jeane Hester and Elihu Estey and Martin Korbling and Nicoletta Pollicardo and Ronald Berenson and Shelly Heimfeld and Richard Champlin and Deisseroth, {Albert B.}",
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T1 - Percentage of Philadelphia Chromosome (Ph)-Negative and Ph-Positive Cells Found after Autologous Transplantation for Chronic Myelogenous Leukemia Depends on Percentage of Diploid Cells Induced by Conventional-Dose Chemotherapy before Collection of Autologous Cells

AU - Talpaz, Moshe

AU - Kantarjian, Hagop

AU - Liang, Jan

AU - Calvert, Leslie

AU - Hamer, Joy

AU - Tibbits, Patricia

AU - Durett, April

AU - Claxton, David

AU - Giralt, Sergio

AU - Khouri, Issa

AU - Przepiorka, Donna

AU - Van Besien, Koen

AU - Andersson, Borje

AU - Mehra, Rakesh

AU - Gajewski, James

AU - Seong, David

AU - Hester, Jeane

AU - Estey, Elihu

AU - Korbling, Martin

AU - Pollicardo, Nicoletta

AU - Berenson, Ronald

AU - Heimfeld, Shelly

AU - Champlin, Richard

AU - Deisseroth, Albert B.

PY - 1995/6/1

Y1 - 1995/6/1

N2 - We collected peripheral blood mononuclear cells and bone marrow cells soon after recovery from conventional-dose chemotherapy-induced myelosuppression and transplanted these cells into advanced chronic myelogenous leukemia (CML) patients after treating these patients with 120 mg/kg cyclophosphamide, 750 mg/m2 VP-16, and 1,020 cGy of total body irradiation (TBI). Of the 10 late chronic-phase patients and the eight accelerated-phase CML patients evaluable posttransplant, 90% and 87%, respectively, remain alive posttransplant, whereas none of the three blast crisis CML patients given this therapy remain alive posttransplant. We measured the percentage of Philadelphia chromosome (Ph)-negative cells in the autologous cells collected after conventional-dose chemotherapy-induced myelosuppression before autologous transplant and in the marrow of these same CML patients after autologous transplantation of these cells into recipients treated with the cyclophosphamide, VP-16, and TBI. A direct correlation (correlation coefficient = 0.91) was observed between the level of Ph+ cells in the transplanted cells and the percentage of Ph+ marrow cells after transplant in 21 patients so transplanted. The data show that the chance of generating cytogenetic remissions posttransplant depends on the percentage of diploid cells in the preparations of autologous cells used for transplant and the stage of disease of the patients at the time of collection of the autologous cells.

AB - We collected peripheral blood mononuclear cells and bone marrow cells soon after recovery from conventional-dose chemotherapy-induced myelosuppression and transplanted these cells into advanced chronic myelogenous leukemia (CML) patients after treating these patients with 120 mg/kg cyclophosphamide, 750 mg/m2 VP-16, and 1,020 cGy of total body irradiation (TBI). Of the 10 late chronic-phase patients and the eight accelerated-phase CML patients evaluable posttransplant, 90% and 87%, respectively, remain alive posttransplant, whereas none of the three blast crisis CML patients given this therapy remain alive posttransplant. We measured the percentage of Philadelphia chromosome (Ph)-negative cells in the autologous cells collected after conventional-dose chemotherapy-induced myelosuppression before autologous transplant and in the marrow of these same CML patients after autologous transplantation of these cells into recipients treated with the cyclophosphamide, VP-16, and TBI. A direct correlation (correlation coefficient = 0.91) was observed between the level of Ph+ cells in the transplanted cells and the percentage of Ph+ marrow cells after transplant in 21 patients so transplanted. The data show that the chance of generating cytogenetic remissions posttransplant depends on the percentage of diploid cells in the preparations of autologous cells used for transplant and the stage of disease of the patients at the time of collection of the autologous cells.

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