Penetrance of congenital heart disease in a mouse model of down syndrome depends on a trisomic potentiator of a disomic modifier

Huiqing Li, Sarah Edie, Donna Klinedinst, Jun Seop Jeong, Seth Blackshaw, Cheryl Maslen, Roger H. Reeves

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Down syndrome (DS) is a significant risk factor for congenital heart disease (CHD), increasing the incidence 50 times over the general population. However, half of people with DS have a normal heart and thus trisomy 21 is not sufficient to cause CHD by itself. Ts65Dn mice are trisomic for orthologs of >100 Hsa21 genes, and their heart defect frequency is significantly higher than their euploid littermates. Introduction of a null allele of Creld1 into Ts65Dn increases the penetrance of heart defects significantly. However, this increase was not seen when the Creld1 null allele was introduced into Ts1Cje, a mouse that is trisomic for about two thirds of the Hsa21 orthologs that are triplicated in Ts65Dn. Among the 23 genes present in three copies in Ts65Dn but not Ts1Cje, we identified Jam2 as necessary for the increased penetrance of Creld1-mediated septal defects in Ts65Dn. Thus, overexpression of the trisomic gene, Jam2, is a necessary potentiator of the disomic genetic modifier, Creld1. No direct physical interaction between Jam2 and Creld1 was identified by several methods. Regions of Hsa21 containing genes that are risk factors of CHD have been identified, but Jam2 (and its environs) has not been linked to heart formation previously. The complexity of this interaction may be more representative of the clinical situation in people than consideration of simple single-gene models.

    Original languageEnglish (US)
    Pages (from-to)763-770
    Number of pages8
    JournalGenetics
    Volume203
    Issue number2
    DOIs
    StatePublished - Jun 1 2016

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    Penetrance
    Down Syndrome
    Heart Diseases
    Genes
    Alleles
    Incidence
    Population

    Keywords

    • Congenital heart disease
    • Disomic modifier
    • Down syndrome
    • Trisomic potentiator

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Penetrance of congenital heart disease in a mouse model of down syndrome depends on a trisomic potentiator of a disomic modifier. / Li, Huiqing; Edie, Sarah; Klinedinst, Donna; Jeong, Jun Seop; Blackshaw, Seth; Maslen, Cheryl; Reeves, Roger H.

    In: Genetics, Vol. 203, No. 2, 01.06.2016, p. 763-770.

    Research output: Contribution to journalArticle

    Li, Huiqing ; Edie, Sarah ; Klinedinst, Donna ; Jeong, Jun Seop ; Blackshaw, Seth ; Maslen, Cheryl ; Reeves, Roger H. / Penetrance of congenital heart disease in a mouse model of down syndrome depends on a trisomic potentiator of a disomic modifier. In: Genetics. 2016 ; Vol. 203, No. 2. pp. 763-770.
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