PAX2 expression in wilms tumors and other childhood neoplasms

Jessica Davis, Linh Matsumura, Douglas Weeks, Megan Troxell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

PAX2 plays an important role in kidney development; although small studies have demonstrated PAX2 expression in Wilms tumors (WT), comprehensive studies on formalin-fixed tissue are lacking. Thus, we systematically evaluated PAX2 immunohistochemical staining in a retrospective study of pediatric WT, as compared with other pediatric tumors. We stained formalin-fixed, paraffin-embedded sections from 39 WT, 6 nephrogenic rests, 8 non-Wilms renal tumors, and 43 nonrenal pediatric small round cell tumors with 2 different PAX2 polyclonal antibodies. PAX2 demonstrated strong, diffuse staining of epithelial and blastema components of WT (97% of cases). PAX2 stained WT stroma in fewer cases (23%), but 80% of anaplastic foci were positive. Nephrogenic rests, 1 case of metanephric adenoma, and 1 pediatric renal cell carcinoma were also PAX2 positive; other pediatric renal tumors were negative. Neuroblastoma, primitive neuroectodermal tumor/Ewings, and T-cell acute lymphoblastic lymphoma (ALL) were PAX2 negative. However, PAX2 weakly stained some cases of B-cell ALL rhabdomyosarcoma (RMS) was also stained, especially alveolar RMS (83%), with less staining of embryonal RMS (13%). One of the antibodies also stained maturing myoid cytoplasm of WT and RMS. This study shows that PAX2 is a sensitive marker of WT (sensitivity 97%), but PAX2 shows weak-to-moderate- intensity nuclear staining of RMS and B-cell ALL, somewhat limiting its utility. However, PAX2 may be a helpful marker in certain diagnostic situations. We speculate that RMS and B-cell ALL staining could be due to antibody cross-reactivity with PAX family members with known expression in RMS and B-cell ALL.

Original languageEnglish (US)
Pages (from-to)1186-1194
Number of pages9
JournalAmerican Journal of Surgical Pathology
Volume35
Issue number8
DOIs
StatePublished - Aug 2011

Fingerprint

Wilms Tumor
Rhabdomyosarcoma
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Pediatrics
Staining and Labeling
B-Lymphocytes
Neoplasms
Kidney
Formaldehyde
Antibodies
Alveolar Rhabdomyosarcoma
Embryonal Rhabdomyosarcoma
Primitive Neuroectodermal Tumors
Neuroblastoma
Adenoma
Paraffin
Cytoplasm
Retrospective Studies
T-Lymphocytes

Keywords

  • ALL
  • PAX2
  • rhabdomyosarcoma
  • Wilms tumor

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

PAX2 expression in wilms tumors and other childhood neoplasms. / Davis, Jessica; Matsumura, Linh; Weeks, Douglas; Troxell, Megan.

In: American Journal of Surgical Pathology, Vol. 35, No. 8, 08.2011, p. 1186-1194.

Research output: Contribution to journalArticle

Davis, Jessica ; Matsumura, Linh ; Weeks, Douglas ; Troxell, Megan. / PAX2 expression in wilms tumors and other childhood neoplasms. In: American Journal of Surgical Pathology. 2011 ; Vol. 35, No. 8. pp. 1186-1194.
@article{f0df69a7c8d1478d85484ad2114d8b47,
title = "PAX2 expression in wilms tumors and other childhood neoplasms",
abstract = "PAX2 plays an important role in kidney development; although small studies have demonstrated PAX2 expression in Wilms tumors (WT), comprehensive studies on formalin-fixed tissue are lacking. Thus, we systematically evaluated PAX2 immunohistochemical staining in a retrospective study of pediatric WT, as compared with other pediatric tumors. We stained formalin-fixed, paraffin-embedded sections from 39 WT, 6 nephrogenic rests, 8 non-Wilms renal tumors, and 43 nonrenal pediatric small round cell tumors with 2 different PAX2 polyclonal antibodies. PAX2 demonstrated strong, diffuse staining of epithelial and blastema components of WT (97{\%} of cases). PAX2 stained WT stroma in fewer cases (23{\%}), but 80{\%} of anaplastic foci were positive. Nephrogenic rests, 1 case of metanephric adenoma, and 1 pediatric renal cell carcinoma were also PAX2 positive; other pediatric renal tumors were negative. Neuroblastoma, primitive neuroectodermal tumor/Ewings, and T-cell acute lymphoblastic lymphoma (ALL) were PAX2 negative. However, PAX2 weakly stained some cases of B-cell ALL rhabdomyosarcoma (RMS) was also stained, especially alveolar RMS (83{\%}), with less staining of embryonal RMS (13{\%}). One of the antibodies also stained maturing myoid cytoplasm of WT and RMS. This study shows that PAX2 is a sensitive marker of WT (sensitivity 97{\%}), but PAX2 shows weak-to-moderate- intensity nuclear staining of RMS and B-cell ALL, somewhat limiting its utility. However, PAX2 may be a helpful marker in certain diagnostic situations. We speculate that RMS and B-cell ALL staining could be due to antibody cross-reactivity with PAX family members with known expression in RMS and B-cell ALL.",
keywords = "ALL, PAX2, rhabdomyosarcoma, Wilms tumor",
author = "Jessica Davis and Linh Matsumura and Douglas Weeks and Megan Troxell",
year = "2011",
month = "8",
doi = "10.1097/PAS.0b013e31821d3131",
language = "English (US)",
volume = "35",
pages = "1186--1194",
journal = "American Journal of Surgical Pathology",
issn = "0147-5185",
publisher = "Lippincott Williams and Wilkins",
number = "8",

}

TY - JOUR

T1 - PAX2 expression in wilms tumors and other childhood neoplasms

AU - Davis, Jessica

AU - Matsumura, Linh

AU - Weeks, Douglas

AU - Troxell, Megan

PY - 2011/8

Y1 - 2011/8

N2 - PAX2 plays an important role in kidney development; although small studies have demonstrated PAX2 expression in Wilms tumors (WT), comprehensive studies on formalin-fixed tissue are lacking. Thus, we systematically evaluated PAX2 immunohistochemical staining in a retrospective study of pediatric WT, as compared with other pediatric tumors. We stained formalin-fixed, paraffin-embedded sections from 39 WT, 6 nephrogenic rests, 8 non-Wilms renal tumors, and 43 nonrenal pediatric small round cell tumors with 2 different PAX2 polyclonal antibodies. PAX2 demonstrated strong, diffuse staining of epithelial and blastema components of WT (97% of cases). PAX2 stained WT stroma in fewer cases (23%), but 80% of anaplastic foci were positive. Nephrogenic rests, 1 case of metanephric adenoma, and 1 pediatric renal cell carcinoma were also PAX2 positive; other pediatric renal tumors were negative. Neuroblastoma, primitive neuroectodermal tumor/Ewings, and T-cell acute lymphoblastic lymphoma (ALL) were PAX2 negative. However, PAX2 weakly stained some cases of B-cell ALL rhabdomyosarcoma (RMS) was also stained, especially alveolar RMS (83%), with less staining of embryonal RMS (13%). One of the antibodies also stained maturing myoid cytoplasm of WT and RMS. This study shows that PAX2 is a sensitive marker of WT (sensitivity 97%), but PAX2 shows weak-to-moderate- intensity nuclear staining of RMS and B-cell ALL, somewhat limiting its utility. However, PAX2 may be a helpful marker in certain diagnostic situations. We speculate that RMS and B-cell ALL staining could be due to antibody cross-reactivity with PAX family members with known expression in RMS and B-cell ALL.

AB - PAX2 plays an important role in kidney development; although small studies have demonstrated PAX2 expression in Wilms tumors (WT), comprehensive studies on formalin-fixed tissue are lacking. Thus, we systematically evaluated PAX2 immunohistochemical staining in a retrospective study of pediatric WT, as compared with other pediatric tumors. We stained formalin-fixed, paraffin-embedded sections from 39 WT, 6 nephrogenic rests, 8 non-Wilms renal tumors, and 43 nonrenal pediatric small round cell tumors with 2 different PAX2 polyclonal antibodies. PAX2 demonstrated strong, diffuse staining of epithelial and blastema components of WT (97% of cases). PAX2 stained WT stroma in fewer cases (23%), but 80% of anaplastic foci were positive. Nephrogenic rests, 1 case of metanephric adenoma, and 1 pediatric renal cell carcinoma were also PAX2 positive; other pediatric renal tumors were negative. Neuroblastoma, primitive neuroectodermal tumor/Ewings, and T-cell acute lymphoblastic lymphoma (ALL) were PAX2 negative. However, PAX2 weakly stained some cases of B-cell ALL rhabdomyosarcoma (RMS) was also stained, especially alveolar RMS (83%), with less staining of embryonal RMS (13%). One of the antibodies also stained maturing myoid cytoplasm of WT and RMS. This study shows that PAX2 is a sensitive marker of WT (sensitivity 97%), but PAX2 shows weak-to-moderate- intensity nuclear staining of RMS and B-cell ALL, somewhat limiting its utility. However, PAX2 may be a helpful marker in certain diagnostic situations. We speculate that RMS and B-cell ALL staining could be due to antibody cross-reactivity with PAX family members with known expression in RMS and B-cell ALL.

KW - ALL

KW - PAX2

KW - rhabdomyosarcoma

KW - Wilms tumor

UR - http://www.scopus.com/inward/record.url?scp=79961021878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961021878&partnerID=8YFLogxK

U2 - 10.1097/PAS.0b013e31821d3131

DO - 10.1097/PAS.0b013e31821d3131

M3 - Article

VL - 35

SP - 1186

EP - 1194

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

SN - 0147-5185

IS - 8

ER -