Pathogenicity in mice of herpes simplex virus type 2 mutants unable to express glycoprotein C

D. C. Johnson, M. R. McDermott, C. Chrisp, J. C. Glorioso

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Herpes simplex virus type 2 (HSV-2) mutants that were unable to express glycoprotein C (gC-2) were isolated. Deletions were made in a cloned copy of the gC-2 gene, and recombinant viruses containing these deletions were screened by using an immunoreactive plaque selection protocol. The viruses did not display a syncytial phenotype. Intravaginal inoculation of BALB/cJ mice with one of the HSV-2 gC-2- viruses produced local inflammation followed by a lethal spread of the viral infection into the nervous system in a manner identical to that produced by parental HSV-2 strain 333. Similarly, intracerebral inoculation of DBA-2 mice with the gC-2- virus produced a lethal neurological disease paralelling that caused by HSV-2 strain 333. These results indicate that gC-2 is not required for the spread of HSV-2 infections in mice.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalJournal of virology
Issue number1
StatePublished - Jan 1 1986


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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