PASTA: PARP activity screening and inhibitor testing assay

Ilsa T. Kirby, Daniel J. Sanderson, Michael S. Cohen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).

Original languageEnglish (US)
Article number100344
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021
Externally publishedYes

Keywords

  • High-throughput screening
  • Molecular/chemical probes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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