Passiflora incarnata L. (Passionflower) extracts elicit GABA currents in hippocampal neurons in vitro, and show anxiogenic and anticonvulsant effects in vivo, varying with extraction method

S. M. Elsas, D. J. Rossi, Jacob Raber, G. White, C. A. Seeley, W. L. Gregory, C. Mohr, T. Pfankuch, Amala Soumyanath

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Potential mechanisms of Passiflora incarnata extracts and the effect of extraction methods on ingredients and biological effects were explored. Using the same batch of plant material, total flavonoid yields as measured by high-performance liquid chromatography coupled to diode array detection (HPLC-DAD) increased substantially with hot versus cold extraction methods.Whole Passiflora extract induced prominent, dose-dependent direct GABAA currents in hippocampal slices, but the expected modulation of synaptic GABAA currents was not seen. GABA was found to be a prominent ingredient of Passiflora extract, and GABA currents were absent when amino acids were removed from the extract.Five different extracts, prepared from a single batch of Passiflora incarnata, were administered to CF-1 mice for 1 week in their drinking water prior to evaluation of their behavioral effects. Anticonvulsant effects against PTZ-induced seizures were seen in mice that received 2 of the 5 Passiflora extracts. Instead of the anxiolytic effects described by others, anxiogenic effects in the elevated plus maze were seen in mice receiving any of the 5 Passiflora extracts.

Original languageEnglish (US)
Pages (from-to)940-949
Number of pages10
JournalPhytomedicine
Volume17
Issue number12
DOIs
StatePublished - Oct 2010

Fingerprint

Passiflora
Anticonvulsants
gamma-Aminobutyric Acid
Neurons
Anti-Anxiety Agents
Flavonoids
Drinking Water
In Vitro Techniques
Seizures
High Pressure Liquid Chromatography
Amino Acids

Keywords

  • Elevated plus maze
  • Epileptic seizure
  • Flavonoid
  • GABA receptor
  • Passian flower
  • Passiflora incarnata
  • Pentylenetetrazol
  • Rotarod

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Passiflora incarnata L. (Passionflower) extracts elicit GABA currents in hippocampal neurons in vitro, and show anxiogenic and anticonvulsant effects in vivo, varying with extraction method. / Elsas, S. M.; Rossi, D. J.; Raber, Jacob; White, G.; Seeley, C. A.; Gregory, W. L.; Mohr, C.; Pfankuch, T.; Soumyanath, Amala.

In: Phytomedicine, Vol. 17, No. 12, 10.2010, p. 940-949.

Research output: Contribution to journalArticle

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