Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function

Michael P. Sarras, Dennis R. Trune, Elaine M. Merisko, Floyd M. Foltz, Stanley R. Nelson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The relationship of the paraphyseal-choroid plexus complex to parathyroid gland function was investigated in adult frogs. Light microscopy and morphometric analysis indicated that total parathyroid gland volume, cell volume and vascular volume doubled by 7-28 days after surgical removal of the paraphyseal-choroid plexus complex (paraphysectomy). This increase correlated with the appearance of (1) large Golgi-associated vesicles, (2) an increase in the apparent number of cytoplasmic dense-core granules, and (3) PTH within the parenchymal cells as monitored by imrnunofluorescence. Twelve months after paraphysectomy, parathyroid glands became cystic with a central fluid-filled cavity surrounded by a stratified cuboidal cell layer. The parenchymal cells of cystic glands contained numerous cytoplasmic dense-core granules and were also positive for PTH. Radioimmunoassay of cystic parathyroid fluid indicated a PTH concentration of 2 μg/μ1; however, analysis by SDS-PAGE indicated a wide range of proteins in cystic fluid. The results of this study indicate that paraphysectomy induces stimulation of the parathyroid glands and suggest a role for the paraphyseal-choroid plexus complex in the regulation of amphibian parathyroid gland function.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume47
Issue number1-2
DOIs
StatePublished - Sep 1986

Keywords

  • Ca regulation
  • amphibian endocrine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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