Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function

Michael P. Sarras; Dennis R. Trune; Elaine M. Merisko; Floyd M. Foltz; Stanley R. Nelson

doi:10.1016/0303-7207(86)90016-X

Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function

Michael P. Sarras, Dennis R. Trune, Elaine M. Merisko, Floyd M. Foltz, Stanley R. Nelson

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The relationship of the paraphyseal-choroid plexus complex to parathyroid gland function was investigated in adult frogs. Light microscopy and morphometric analysis indicated that total parathyroid gland volume, cell volume and vascular volume doubled by 7-28 days after surgical removal of the paraphyseal-choroid plexus complex (paraphysectomy). This increase correlated with the appearance of (1) large Golgi-associated vesicles, (2) an increase in the apparent number of cytoplasmic dense-core granules, and (3) PTH within the parenchymal cells as monitored by imrnunofluorescence. Twelve months after paraphysectomy, parathyroid glands became cystic with a central fluid-filled cavity surrounded by a stratified cuboidal cell layer. The parenchymal cells of cystic glands contained numerous cytoplasmic dense-core granules and were also positive for PTH. Radioimmunoassay of cystic parathyroid fluid indicated a PTH concentration of 2 μg/μ1; however, analysis by SDS-PAGE indicated a wide range of proteins in cystic fluid. The results of this study indicate that paraphysectomy induces stimulation of the parathyroid glands and suggest a role for the paraphyseal-choroid plexus complex in the regulation of amphibian parathyroid gland function.

Original language	English (US)
Pages (from-to)	59-70
Number of pages	12
Journal	Molecular and Cellular Endocrinology
Volume	47
Issue number	1-2
DOIs	https://doi.org/10.1016/0303-7207(86)90016-X
State	Published - Sep 1986
Externally published	Yes

Keywords

Ca regulation
amphibian endocrine

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(86)90016-X

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Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function. / Sarras, Michael P.; Trune, Dennis R.; Merisko, Elaine M. et al.
In: Molecular and Cellular Endocrinology, Vol. 47, No. 1-2, 09.1986, p. 59-70.

Research output: Contribution to journal › Article › peer-review

@article{488151cc1821473bb310b1d424db65d5,

title = "Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function",

abstract = "The relationship of the paraphyseal-choroid plexus complex to parathyroid gland function was investigated in adult frogs. Light microscopy and morphometric analysis indicated that total parathyroid gland volume, cell volume and vascular volume doubled by 7-28 days after surgical removal of the paraphyseal-choroid plexus complex (paraphysectomy). This increase correlated with the appearance of (1) large Golgi-associated vesicles, (2) an increase in the apparent number of cytoplasmic dense-core granules, and (3) PTH within the parenchymal cells as monitored by imrnunofluorescence. Twelve months after paraphysectomy, parathyroid glands became cystic with a central fluid-filled cavity surrounded by a stratified cuboidal cell layer. The parenchymal cells of cystic glands contained numerous cytoplasmic dense-core granules and were also positive for PTH. Radioimmunoassay of cystic parathyroid fluid indicated a PTH concentration of 2 μg/μ1; however, analysis by SDS-PAGE indicated a wide range of proteins in cystic fluid. The results of this study indicate that paraphysectomy induces stimulation of the parathyroid glands and suggest a role for the paraphyseal-choroid plexus complex in the regulation of amphibian parathyroid gland function.",

keywords = "Ca regulation, amphibian endocrine",

author = "Sarras, {Michael P.} and Trune, {Dennis R.} and Merisko, {Elaine M.} and Foltz, {Floyd M.} and Nelson, {Stanley R.}",

note = "Funding Information: The authorsw ish to thank Dr. Ronal R. Macgregor of the Veterans Administration Medical Center,K ansasC ity, MO, for kindly providingt he antibodyu sedi n this studya nd for conductingt he PTH radioimmunoassayIsn. addition,t he authors also wish to thank Larry Nolar, Jacquelyn Huff, Lisa Brown, Airlean Bowls and Rosetta Barkley for their assistancew ith light microscopy. The authorsa lso thank Ms. BrendaT ennyson-Jackson for her help in the preparationo f this manuscript. This researchw as supportedi n part from funds from the Amyotrophic Lateral SclerosisS ocietyo f America (S.R.N.) and from the USPHS, NIH Grant AM33362 (M.P.S.).",

year = "1986",

month = sep,

doi = "10.1016/0303-7207(86)90016-X",

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T1 - Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana)

T2 - Evidence for telencephalic regulation of parathyroid gland function

AU - Sarras, Michael P.

AU - Trune, Dennis R.

AU - Merisko, Elaine M.

AU - Foltz, Floyd M.

AU - Nelson, Stanley R.

N1 - Funding Information: The authorsw ish to thank Dr. Ronal R. Macgregor of the Veterans Administration Medical Center,K ansasC ity, MO, for kindly providingt he antibodyu sedi n this studya nd for conductingt he PTH radioimmunoassayIsn. addition,t he authors also wish to thank Larry Nolar, Jacquelyn Huff, Lisa Brown, Airlean Bowls and Rosetta Barkley for their assistancew ith light microscopy. The authorsa lso thank Ms. BrendaT ennyson-Jackson for her help in the preparationo f this manuscript. This researchw as supportedi n part from funds from the Amyotrophic Lateral SclerosisS ocietyo f America (S.R.N.) and from the USPHS, NIH Grant AM33362 (M.P.S.).

PY - 1986/9

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N2 - The relationship of the paraphyseal-choroid plexus complex to parathyroid gland function was investigated in adult frogs. Light microscopy and morphometric analysis indicated that total parathyroid gland volume, cell volume and vascular volume doubled by 7-28 days after surgical removal of the paraphyseal-choroid plexus complex (paraphysectomy). This increase correlated with the appearance of (1) large Golgi-associated vesicles, (2) an increase in the apparent number of cytoplasmic dense-core granules, and (3) PTH within the parenchymal cells as monitored by imrnunofluorescence. Twelve months after paraphysectomy, parathyroid glands became cystic with a central fluid-filled cavity surrounded by a stratified cuboidal cell layer. The parenchymal cells of cystic glands contained numerous cytoplasmic dense-core granules and were also positive for PTH. Radioimmunoassay of cystic parathyroid fluid indicated a PTH concentration of 2 μg/μ1; however, analysis by SDS-PAGE indicated a wide range of proteins in cystic fluid. The results of this study indicate that paraphysectomy induces stimulation of the parathyroid glands and suggest a role for the paraphyseal-choroid plexus complex in the regulation of amphibian parathyroid gland function.

AB - The relationship of the paraphyseal-choroid plexus complex to parathyroid gland function was investigated in adult frogs. Light microscopy and morphometric analysis indicated that total parathyroid gland volume, cell volume and vascular volume doubled by 7-28 days after surgical removal of the paraphyseal-choroid plexus complex (paraphysectomy). This increase correlated with the appearance of (1) large Golgi-associated vesicles, (2) an increase in the apparent number of cytoplasmic dense-core granules, and (3) PTH within the parenchymal cells as monitored by imrnunofluorescence. Twelve months after paraphysectomy, parathyroid glands became cystic with a central fluid-filled cavity surrounded by a stratified cuboidal cell layer. The parenchymal cells of cystic glands contained numerous cytoplasmic dense-core granules and were also positive for PTH. Radioimmunoassay of cystic parathyroid fluid indicated a PTH concentration of 2 μg/μ1; however, analysis by SDS-PAGE indicated a wide range of proteins in cystic fluid. The results of this study indicate that paraphysectomy induces stimulation of the parathyroid glands and suggest a role for the paraphyseal-choroid plexus complex in the regulation of amphibian parathyroid gland function.

KW - Ca regulation

KW - amphibian endocrine

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SN - 0303-7207

VL - 47

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JO - Molecular and Cellular Endocrinology

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Paraphysectomy-induced stimulation of parathyroid glands in mature frogs (Rana catesbeiana): Evidence for telencephalic regulation of parathyroid gland function

Abstract

Keywords

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