Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography

Loretta Scolaro, Robert A. McLaughlin, Blake R. Klyen, Benjamin A. Wood, Peter D. Robbins, Christobel M. Saunders, Steven L. Jacques, David D. Sampson

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

We report the use of optical coherence tomography (OCT) to determine spatially localized optical attenuation coefficients of human axillary lymph nodes and their use to generate parametric images of lymphoid tissue. 3D-OCT images were obtained from excised lymph nodes and optical attenuation coefficients were extracted assuming a single scattering model of OCT. We present the measured attenuation coefficients for several tissue regions in benign and reactive lymph nodes, as identified by histopathology. We show parametric images of the measured attenuation coefficients as well as segmented images of tissue type based on thresholding of the attenuation coefficient values. Comparison to histology demonstrates the enhancement of contrast in parametric images relative to OCT images. This enhancement is a step towards the use of OCT for in situ assessment of lymph nodes.

Original languageEnglish (US)
Pages (from-to)366-379
Number of pages14
JournalBiomedical Optics Express
Volume3
Issue number2
DOIs
StatePublished - Feb 1 2012

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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    Scolaro, L., McLaughlin, R. A., Klyen, B. R., Wood, B. A., Robbins, P. D., Saunders, C. M., Jacques, S. L., & Sampson, D. D. (2012). Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography. Biomedical Optics Express, 3(2), 366-379. https://doi.org/10.1364/BOE.3.000366