Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent

Daniele Gerion, Julie Herberg, Robert Bok, Erica Gjersing, Erick Ramon, Robert Maxwell, John Kurhanewicz, Thomas F. Budinger, Joe Gray, Marc A. Shuman, Fanqing Frank Chen

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

We present a robust and general method for embedding nanoparticles, such as quantum dots (QD) or colloidal gold (Au) nanocrystals, into a highly water-soluble thin silica shell doped with paramagnetic gadolinium (Gd 3+) ions without negatively impacting the optical properties of the QD or Au nanoparticle cores. The ultrathin silica shell has been covalently linked to Gd3+ ions chelator, tetraazacyclododecanetetraacetic acid (DOTA). The resulting complex has a diameter of 8 to 15 nm and is soluble in high ionic strength buffers at pH values ranging from approximately 4 to 11. For this system, nanoparticle concentrations exceed 50 μM, while most other nanoparticles might aggregate. In magnetic resonance imaging (MRI) experiments at clinical magnetic field strengths of 1.4 T (1H resonance frequency of 60 MHz), the gadolinium-DOTA (Gd-DOTA) attached to SiO2-coated QDs has a spin-lattice (T1) particle relaxivity () and a spin-spin (T2) particle relaxivity (r2) of 1019 ±19 mM -1s-1 and 2438 ±46 mM-1 s-1, respectively, for a 8-nm QD. The particle relaxivity has been correlated to the number of Gd3+ covalently linked to the silica shell. At 1.4 T, the Gd-DOTA ion relaxivities, r1 and r2, respectively, are 23 ±0.40 mM-1s-1 and 54 ±1.0 mM -1s-1. The sensitivity of our probes is in the 100-nM range for 8-10 nm particles and reaches 10 nM for particles approximately 15 nm in diameter. Preliminary dynamic contrast enhancement MRI experiments in mice revealed that silica-coated MRI probes are cleared from the renal system into the bladder with no observable affects on the health of the animal. This current approach may offer numerous advantages over other similar approaches,12 including greater relaxivity and greater simplicity for the synthesis process of dual modality contrast agents that allow both MRI and optical detection as well as applicability to other nanoparticles.

Original languageEnglish (US)
Pages (from-to)12542-12551
Number of pages10
JournalJournal of Physical Chemistry C
Volume111
Issue number34
DOIs
StatePublished - Aug 30 2007
Externally publishedYes

Fingerprint

Magnetic resonance
Silicon Dioxide
Contrast Media
magnetic resonance
Silica
Gadolinium
Nanoparticles
silicon dioxide
Imaging techniques
nanoparticles
gadolinium
Semiconductor quantum dots
quantum dots
Ions
Gold Colloid
particle spin
ions
probes
bladder
Chelating Agents

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Gerion, D., Herberg, J., Bok, R., Gjersing, E., Ramon, E., Maxwell, R., ... Chen, F. F. (2007). Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent. Journal of Physical Chemistry C, 111(34), 12542-12551. https://doi.org/10.1021/jp074072p

Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent. / Gerion, Daniele; Herberg, Julie; Bok, Robert; Gjersing, Erica; Ramon, Erick; Maxwell, Robert; Kurhanewicz, John; Budinger, Thomas F.; Gray, Joe; Shuman, Marc A.; Chen, Fanqing Frank.

In: Journal of Physical Chemistry C, Vol. 111, No. 34, 30.08.2007, p. 12542-12551.

Research output: Contribution to journalArticle

Gerion, D, Herberg, J, Bok, R, Gjersing, E, Ramon, E, Maxwell, R, Kurhanewicz, J, Budinger, TF, Gray, J, Shuman, MA & Chen, FF 2007, 'Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent', Journal of Physical Chemistry C, vol. 111, no. 34, pp. 12542-12551. https://doi.org/10.1021/jp074072p
Gerion D, Herberg J, Bok R, Gjersing E, Ramon E, Maxwell R et al. Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent. Journal of Physical Chemistry C. 2007 Aug 30;111(34):12542-12551. https://doi.org/10.1021/jp074072p
Gerion, Daniele ; Herberg, Julie ; Bok, Robert ; Gjersing, Erica ; Ramon, Erick ; Maxwell, Robert ; Kurhanewicz, John ; Budinger, Thomas F. ; Gray, Joe ; Shuman, Marc A. ; Chen, Fanqing Frank. / Paramagnetic silica-coated nangcrystals as an advanced MRI contrast agent. In: Journal of Physical Chemistry C. 2007 ; Vol. 111, No. 34. pp. 12542-12551.
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