PARalyzer: Definition of RNA binding sites from PAR-CLIP short-read sequence data

David L. Corcoran, Stoyan Georgiev, Neelanjan Mukherjee, Eva Gottwein, Rebecca L. Skalsky, Jack D. Keene, Uwe Ohler

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

Crosslinking and immunoprecipitation (CLIP) protocols have made it possible to identify transcriptome-wide RNA-protein interaction sites. In particular, PAR-CLIP utilizes a photoactivatable nucleoside for more efficient crosslinking. We present an approach, centered on the novel PARalyzer tool, for mapping high-confidence sites from PAR-CLIP deep-sequencing data. We show that PARalyzer delineates sites with a high signal-to-noise ratio. Motif finding identifies the sequence preferences of RNA-binding proteins, as well as seed-matches for highly expressed microRNAs when profiling Argonaute proteins. Our study describes tailored analytical methods and provides guidelines for future efforts to utilize high-throughput sequencing in RNA biology.

Original languageEnglish (US)
Article numberR79
JournalGenome biology
Volume12
Issue number8
DOIs
StatePublished - Aug 18 2011

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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    Corcoran, D. L., Georgiev, S., Mukherjee, N., Gottwein, E., Skalsky, R. L., Keene, J. D., & Ohler, U. (2011). PARalyzer: Definition of RNA binding sites from PAR-CLIP short-read sequence data. Genome biology, 12(8), [R79]. https://doi.org/10.1186/gb-2011-12-8-r79