Parallel gene expression changes in sarcoidosis involving the lacrimal gland, orbital tissue, or blood

James (Jim) Rosenbaum, Dongseok Choi, David Wilson, Hans E. Grossniklaus, Christina (Chris) Harrington, Cailin Sibley, Roger Dailey, John Ng, Eric Steele, Craig N. Czyz, Jill A. Foster, David Tse, Chris Alabiad, Sander Dubovy, Prashant Parekh, Gerald J. Harris, Michael Kazim, Payal Patel, Valerie White, Peter DolmanBobby S. Korn, Don Kikkawa, Deepak P. Edward, Hind Alkatan, Hailah Al-Hussain, R. Patrick Yeatts, Dinesh Selva, Patrick Stauffer, Stephen Planck

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

IMPORTANCE: Sarcoidosis is a major cause of ocular or periocular inflammation. The pathogenesis of sarcoidosis is incompletely understood and diagnosis often requires a biopsy. OBJECTIVE: To determine how gene expression in either orbital adipose tissue or the lacrimal gland affected by sarcoidosis compares with gene expression in other causes of orbital disease and how gene expression in tissue affected by sarcoidosis compares with gene expression in peripheral blood samples obtained from patients with sarcoidosis. DESIGN, SETTING, AND PARTICIPANTS: In a multicenter, international, observational study, gene expression profiling of formalin-fixed biopsy specimens, using GeneChipp U133 Plus 2 microarrays (Affymetrix), was conducted between October 2012 and January 2014 on tissues biopsied from January 2000 through June 2013. Participants included 12 patients with orbital sarcoidosis (7 in adipose tissue; 5 affecting the lacrimal gland) as well as comparable tissue from 6 healthy individuals serving as controls or patients with thyroid eye disease, nonspecific orbital inflammation, or granulomatosis with polyangiitis. In addition, results were compared with gene expression in peripheral blood samples obtained from 12 historical individuals with sarcoidosis. MAIN OUTCOMES AND MEASURES: Significantly differentially expressed transcripts defined as a minimum of a 1.5-fold increase or a comparable decrease and a false discovery rate of P <.05. RESULTS: Signals from 2449 probe sets (transcripts from approximately 1522 genes) were significantly increased in the orbital adipose tissue from patients with sarcoidosis. Signals from 4050 probe sets (approximately 2619 genes) were significantly decreased. Signals from 3069 probe sets (approximately 2001 genes) were significantly higher and 3320 (approximately 2283 genes) were significantly lower in the lacrimal gland for patients with sarcoidosis. Ninety-two probe sets (approximately 69 genes) had significantly elevated signals and 67 probe sets (approximately 56 genes) had significantly lower signals in both orbital tissues and in peripheral blood from patients with sarcoidosis. The transcription factors, interferon-response factor 1, interferon-response factor 2, and nuclear factor κB, were strongly implicated in the expression of messenger RNA upregulated in common in the 3 tissues. CONCLUSIONS AND RELEVANCE: Gene expression in sarcoidosis involving the orbit or lacrimal gland can be distinguished from gene expression patterns in control tissue and overlaps with many transcripts upregulated or downregulated in the peripheral blood of patients with sarcoidosis. These observations suggest that common pathogenic mechanisms contribute to sarcoidosis in different sites. The observations support the hypothesis that a pattern of gene expression profiles could provide diagnostic information in patients with sarcoidosis.

Original languageEnglish (US)
Pages (from-to)770-777
Number of pages8
JournalJAMA Ophthalmology
Volume133
Issue number7
DOIs
StatePublished - Jul 1 2015

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Lacrimal Apparatus
Sarcoidosis
Gene Expression
Genes
Adipose Tissue
Interferons
Orbital Diseases
Inflammation
Biopsy
Granulomatosis with Polyangiitis
Eye Diseases
Thyroid Diseases
Gene Expression Profiling
Orbit
Transcriptome
Formaldehyde
Observational Studies

ASJC Scopus subject areas

  • Ophthalmology
  • Medicine(all)

Cite this

Parallel gene expression changes in sarcoidosis involving the lacrimal gland, orbital tissue, or blood. / Rosenbaum, James (Jim); Choi, Dongseok; Wilson, David; Grossniklaus, Hans E.; Harrington, Christina (Chris); Sibley, Cailin; Dailey, Roger; Ng, John; Steele, Eric; Czyz, Craig N.; Foster, Jill A.; Tse, David; Alabiad, Chris; Dubovy, Sander; Parekh, Prashant; Harris, Gerald J.; Kazim, Michael; Patel, Payal; White, Valerie; Dolman, Peter; Korn, Bobby S.; Kikkawa, Don; Edward, Deepak P.; Alkatan, Hind; Al-Hussain, Hailah; Yeatts, R. Patrick; Selva, Dinesh; Stauffer, Patrick; Planck, Stephen.

In: JAMA Ophthalmology, Vol. 133, No. 7, 01.07.2015, p. 770-777.

Research output: Contribution to journalArticle

Rosenbaum, JJ, Choi, D, Wilson, D, Grossniklaus, HE, Harrington, CC, Sibley, C, Dailey, R, Ng, J, Steele, E, Czyz, CN, Foster, JA, Tse, D, Alabiad, C, Dubovy, S, Parekh, P, Harris, GJ, Kazim, M, Patel, P, White, V, Dolman, P, Korn, BS, Kikkawa, D, Edward, DP, Alkatan, H, Al-Hussain, H, Yeatts, RP, Selva, D, Stauffer, P & Planck, S 2015, 'Parallel gene expression changes in sarcoidosis involving the lacrimal gland, orbital tissue, or blood', JAMA Ophthalmology, vol. 133, no. 7, pp. 770-777. https://doi.org/10.1001/jamaophthalmol.2015.0726
Rosenbaum, James (Jim) ; Choi, Dongseok ; Wilson, David ; Grossniklaus, Hans E. ; Harrington, Christina (Chris) ; Sibley, Cailin ; Dailey, Roger ; Ng, John ; Steele, Eric ; Czyz, Craig N. ; Foster, Jill A. ; Tse, David ; Alabiad, Chris ; Dubovy, Sander ; Parekh, Prashant ; Harris, Gerald J. ; Kazim, Michael ; Patel, Payal ; White, Valerie ; Dolman, Peter ; Korn, Bobby S. ; Kikkawa, Don ; Edward, Deepak P. ; Alkatan, Hind ; Al-Hussain, Hailah ; Yeatts, R. Patrick ; Selva, Dinesh ; Stauffer, Patrick ; Planck, Stephen. / Parallel gene expression changes in sarcoidosis involving the lacrimal gland, orbital tissue, or blood. In: JAMA Ophthalmology. 2015 ; Vol. 133, No. 7. pp. 770-777.
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abstract = "IMPORTANCE: Sarcoidosis is a major cause of ocular or periocular inflammation. The pathogenesis of sarcoidosis is incompletely understood and diagnosis often requires a biopsy. OBJECTIVE: To determine how gene expression in either orbital adipose tissue or the lacrimal gland affected by sarcoidosis compares with gene expression in other causes of orbital disease and how gene expression in tissue affected by sarcoidosis compares with gene expression in peripheral blood samples obtained from patients with sarcoidosis. DESIGN, SETTING, AND PARTICIPANTS: In a multicenter, international, observational study, gene expression profiling of formalin-fixed biopsy specimens, using GeneChipp U133 Plus 2 microarrays (Affymetrix), was conducted between October 2012 and January 2014 on tissues biopsied from January 2000 through June 2013. Participants included 12 patients with orbital sarcoidosis (7 in adipose tissue; 5 affecting the lacrimal gland) as well as comparable tissue from 6 healthy individuals serving as controls or patients with thyroid eye disease, nonspecific orbital inflammation, or granulomatosis with polyangiitis. In addition, results were compared with gene expression in peripheral blood samples obtained from 12 historical individuals with sarcoidosis. MAIN OUTCOMES AND MEASURES: Significantly differentially expressed transcripts defined as a minimum of a 1.5-fold increase or a comparable decrease and a false discovery rate of P <.05. RESULTS: Signals from 2449 probe sets (transcripts from approximately 1522 genes) were significantly increased in the orbital adipose tissue from patients with sarcoidosis. Signals from 4050 probe sets (approximately 2619 genes) were significantly decreased. Signals from 3069 probe sets (approximately 2001 genes) were significantly higher and 3320 (approximately 2283 genes) were significantly lower in the lacrimal gland for patients with sarcoidosis. Ninety-two probe sets (approximately 69 genes) had significantly elevated signals and 67 probe sets (approximately 56 genes) had significantly lower signals in both orbital tissues and in peripheral blood from patients with sarcoidosis. The transcription factors, interferon-response factor 1, interferon-response factor 2, and nuclear factor κB, were strongly implicated in the expression of messenger RNA upregulated in common in the 3 tissues. CONCLUSIONS AND RELEVANCE: Gene expression in sarcoidosis involving the orbit or lacrimal gland can be distinguished from gene expression patterns in control tissue and overlaps with many transcripts upregulated or downregulated in the peripheral blood of patients with sarcoidosis. These observations suggest that common pathogenic mechanisms contribute to sarcoidosis in different sites. The observations support the hypothesis that a pattern of gene expression profiles could provide diagnostic information in patients with sarcoidosis.",
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T1 - Parallel gene expression changes in sarcoidosis involving the lacrimal gland, orbital tissue, or blood

AU - Rosenbaum, James (Jim)

AU - Choi, Dongseok

AU - Wilson, David

AU - Grossniklaus, Hans E.

AU - Harrington, Christina (Chris)

AU - Sibley, Cailin

AU - Dailey, Roger

AU - Ng, John

AU - Steele, Eric

AU - Czyz, Craig N.

AU - Foster, Jill A.

AU - Tse, David

AU - Alabiad, Chris

AU - Dubovy, Sander

AU - Parekh, Prashant

AU - Harris, Gerald J.

AU - Kazim, Michael

AU - Patel, Payal

AU - White, Valerie

AU - Dolman, Peter

AU - Korn, Bobby S.

AU - Kikkawa, Don

AU - Edward, Deepak P.

AU - Alkatan, Hind

AU - Al-Hussain, Hailah

AU - Yeatts, R. Patrick

AU - Selva, Dinesh

AU - Stauffer, Patrick

AU - Planck, Stephen

PY - 2015/7/1

Y1 - 2015/7/1

N2 - IMPORTANCE: Sarcoidosis is a major cause of ocular or periocular inflammation. The pathogenesis of sarcoidosis is incompletely understood and diagnosis often requires a biopsy. OBJECTIVE: To determine how gene expression in either orbital adipose tissue or the lacrimal gland affected by sarcoidosis compares with gene expression in other causes of orbital disease and how gene expression in tissue affected by sarcoidosis compares with gene expression in peripheral blood samples obtained from patients with sarcoidosis. DESIGN, SETTING, AND PARTICIPANTS: In a multicenter, international, observational study, gene expression profiling of formalin-fixed biopsy specimens, using GeneChipp U133 Plus 2 microarrays (Affymetrix), was conducted between October 2012 and January 2014 on tissues biopsied from January 2000 through June 2013. Participants included 12 patients with orbital sarcoidosis (7 in adipose tissue; 5 affecting the lacrimal gland) as well as comparable tissue from 6 healthy individuals serving as controls or patients with thyroid eye disease, nonspecific orbital inflammation, or granulomatosis with polyangiitis. In addition, results were compared with gene expression in peripheral blood samples obtained from 12 historical individuals with sarcoidosis. MAIN OUTCOMES AND MEASURES: Significantly differentially expressed transcripts defined as a minimum of a 1.5-fold increase or a comparable decrease and a false discovery rate of P <.05. RESULTS: Signals from 2449 probe sets (transcripts from approximately 1522 genes) were significantly increased in the orbital adipose tissue from patients with sarcoidosis. Signals from 4050 probe sets (approximately 2619 genes) were significantly decreased. Signals from 3069 probe sets (approximately 2001 genes) were significantly higher and 3320 (approximately 2283 genes) were significantly lower in the lacrimal gland for patients with sarcoidosis. Ninety-two probe sets (approximately 69 genes) had significantly elevated signals and 67 probe sets (approximately 56 genes) had significantly lower signals in both orbital tissues and in peripheral blood from patients with sarcoidosis. The transcription factors, interferon-response factor 1, interferon-response factor 2, and nuclear factor κB, were strongly implicated in the expression of messenger RNA upregulated in common in the 3 tissues. CONCLUSIONS AND RELEVANCE: Gene expression in sarcoidosis involving the orbit or lacrimal gland can be distinguished from gene expression patterns in control tissue and overlaps with many transcripts upregulated or downregulated in the peripheral blood of patients with sarcoidosis. These observations suggest that common pathogenic mechanisms contribute to sarcoidosis in different sites. The observations support the hypothesis that a pattern of gene expression profiles could provide diagnostic information in patients with sarcoidosis.

AB - IMPORTANCE: Sarcoidosis is a major cause of ocular or periocular inflammation. The pathogenesis of sarcoidosis is incompletely understood and diagnosis often requires a biopsy. OBJECTIVE: To determine how gene expression in either orbital adipose tissue or the lacrimal gland affected by sarcoidosis compares with gene expression in other causes of orbital disease and how gene expression in tissue affected by sarcoidosis compares with gene expression in peripheral blood samples obtained from patients with sarcoidosis. DESIGN, SETTING, AND PARTICIPANTS: In a multicenter, international, observational study, gene expression profiling of formalin-fixed biopsy specimens, using GeneChipp U133 Plus 2 microarrays (Affymetrix), was conducted between October 2012 and January 2014 on tissues biopsied from January 2000 through June 2013. Participants included 12 patients with orbital sarcoidosis (7 in adipose tissue; 5 affecting the lacrimal gland) as well as comparable tissue from 6 healthy individuals serving as controls or patients with thyroid eye disease, nonspecific orbital inflammation, or granulomatosis with polyangiitis. In addition, results were compared with gene expression in peripheral blood samples obtained from 12 historical individuals with sarcoidosis. MAIN OUTCOMES AND MEASURES: Significantly differentially expressed transcripts defined as a minimum of a 1.5-fold increase or a comparable decrease and a false discovery rate of P <.05. RESULTS: Signals from 2449 probe sets (transcripts from approximately 1522 genes) were significantly increased in the orbital adipose tissue from patients with sarcoidosis. Signals from 4050 probe sets (approximately 2619 genes) were significantly decreased. Signals from 3069 probe sets (approximately 2001 genes) were significantly higher and 3320 (approximately 2283 genes) were significantly lower in the lacrimal gland for patients with sarcoidosis. Ninety-two probe sets (approximately 69 genes) had significantly elevated signals and 67 probe sets (approximately 56 genes) had significantly lower signals in both orbital tissues and in peripheral blood from patients with sarcoidosis. The transcription factors, interferon-response factor 1, interferon-response factor 2, and nuclear factor κB, were strongly implicated in the expression of messenger RNA upregulated in common in the 3 tissues. CONCLUSIONS AND RELEVANCE: Gene expression in sarcoidosis involving the orbit or lacrimal gland can be distinguished from gene expression patterns in control tissue and overlaps with many transcripts upregulated or downregulated in the peripheral blood of patients with sarcoidosis. These observations suggest that common pathogenic mechanisms contribute to sarcoidosis in different sites. The observations support the hypothesis that a pattern of gene expression profiles could provide diagnostic information in patients with sarcoidosis.

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