Paradoxical contribution of SK3 and GIRK channels to the activation of mouse vomeronasal organ

Sangseong Kim, Limei Ma, Kristi L. Jensen, Michelle M. Kim, Chris T. Bond, John Adelman, C. Ron Yu

Research output: Contribution to journalArticle

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Abstract

The vomeronasal organ (VNO) is essential for intraspecies communication in many terrestrial vertebrates. The ionic mechanisms of VNO activation remain unclear. We found that the calcium-activated potassium channel SK3 and the G proteing-activated potassium channel GIRK are part of an independent pathway for VNO activation. In slice preparations, the potassium channels attenuated inward currents carried by TRPC2 and calcium-activated chloride channels (CACCs). In intact tissue preparations, paradoxically, the potassium channels enhanced urine-evoked inward currents. This discrepancy resulted from the loss of a high concentration of lumenal potassium, which enabled the influx of potassium ions to depolarize the VNO neurons in vivo. Both Sk3 (also known as Kcnn3) and Girk1 (also known as Kcnj3) homozygous null mice showed deficits in mating and aggressive behaviors, and the deficiencies in Sk3 ĝ̂'/ĝ̂' mice were exacerbated by Trpc2 knockout. Our results suggest that VNO activation is mediated by TRPC2, CACCs and two potassium channels, all of which contributed to the in vivo depolarization of VNO neurons.

Original languageEnglish (US)
Pages (from-to)1236-1244
Number of pages9
JournalNature Neuroscience
Volume15
Issue number9
DOIs
StatePublished - Sep 2012

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Vomeronasal Organ
Potassium Channels
Chloride Channels
G Protein-Coupled Inwardly-Rectifying Potassium Channels
Potassium
Neurons
Calcium-Activated Potassium Channels
Vertebrates
Communication
Urine
Ions

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kim, S., Ma, L., Jensen, K. L., Kim, M. M., Bond, C. T., Adelman, J., & Yu, C. R. (2012). Paradoxical contribution of SK3 and GIRK channels to the activation of mouse vomeronasal organ. Nature Neuroscience, 15(9), 1236-1244. https://doi.org/10.1038/nn.3173

Paradoxical contribution of SK3 and GIRK channels to the activation of mouse vomeronasal organ. / Kim, Sangseong; Ma, Limei; Jensen, Kristi L.; Kim, Michelle M.; Bond, Chris T.; Adelman, John; Yu, C. Ron.

In: Nature Neuroscience, Vol. 15, No. 9, 09.2012, p. 1236-1244.

Research output: Contribution to journalArticle

Kim, S, Ma, L, Jensen, KL, Kim, MM, Bond, CT, Adelman, J & Yu, CR 2012, 'Paradoxical contribution of SK3 and GIRK channels to the activation of mouse vomeronasal organ', Nature Neuroscience, vol. 15, no. 9, pp. 1236-1244. https://doi.org/10.1038/nn.3173
Kim, Sangseong ; Ma, Limei ; Jensen, Kristi L. ; Kim, Michelle M. ; Bond, Chris T. ; Adelman, John ; Yu, C. Ron. / Paradoxical contribution of SK3 and GIRK channels to the activation of mouse vomeronasal organ. In: Nature Neuroscience. 2012 ; Vol. 15, No. 9. pp. 1236-1244.
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