Paradox of glucocorticoid-induced cytosolic phospholipase A2 group IVA messenger RNA expression involves glucocorticoid receptor binding to the promoter in human amnion fibroblasts

Chunming Guo, Zhen Yang, Wenjiao Li, Ping Zhu, Leslie Myatt, Kang Sun

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Glucocorticoids (GCs) are well-known anti-inflammatory drugs inhibiting prostaglandin production. Paradoxically, GCs are reported to stimulate cytosolic phosphoplipase A2 group IVA (PLA2G4A) and prostaglandin-endoperoxide synthase 2 (PTGS2) expression in human amnion fibroblasts. This study was designed to examine the molecular mechanisms underlying glucocorticoid-induced PLA2G4A expression in human amnion fibroblasts. Our data showed that cortisol (0.01∼1 μM) increased PLA2G4A mRNA level in a dose-dependent manner in human amnion fibroblasts, which was blocked by glucocorticoid receptor antagonist RU486 (1 μM) as well as by the mRNA transcription inhibitor 5,6-dichlorobenzimidazole riboside (DRB; 75 μM). Concurrently, cortisol (0.01∼1 μM) decreased rather than increased proinflammatory cytokine mRNA levels, including interleukin 1 beta (IL1B), interleukin 6 (IL6), and tumor necrosis factor alpha (TNF), in a dose-dependent manner in human amnion fibroblasts. Chromatin immunoprecipitation assay revealed that glucocorticoid receptor was bound to PLA2G4A promoter in human amnion fibroblasts upon cortisol stimulation. This was confirmed by electrophoretic mobility shift assay showing that nuclear protein extracted from human amnion fibroblasts upon cortisol stimulation could bind the synthesized oligonucleotide sequence corresponding to PLA2G4A promoter region from -95 bp to -65 bp bearing the putative glucocorticoid response element This binding was super shifted by glucocorticoid receptor antibody. In conclusion, we demonstrated in this study that cortisol increased PLA2G4A mRNA level via GR-dependent ongoing transcription in human amnion fibroblasts by activating the binding of GR to PLA2G4A promoter directly, and this effect appeared unlikely to be secondary to the effect of cortisol on the expression of proinflammatory cytokines in human amnion fibroblasts.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalBiology of Reproduction
Volume78
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Fingerprint

Cytosolic Phospholipases A2
Amnion
Glucocorticoid Receptors
Glucocorticoids
Fibroblasts
Hydrocortisone
Messenger RNA
Dichlororibofuranosylbenzimidazole
Cytokines
Chromatin Immunoprecipitation
Response Elements
Electrophoretic Mobility Shift Assay
Prostaglandin-Endoperoxide Synthases
Nuclear Proteins
Interleukin-1beta
varespladib methyl
Genetic Promoter Regions
Oligonucleotides
Prostaglandins
Interleukin-6

Keywords

  • Amnion
  • Cortisol
  • Glucocorticoid receptor
  • Parturition
  • PLA2G4A
  • Placenta
  • Pregnancy
  • Prostaglandin

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Paradox of glucocorticoid-induced cytosolic phospholipase A2 group IVA messenger RNA expression involves glucocorticoid receptor binding to the promoter in human amnion fibroblasts. / Guo, Chunming; Yang, Zhen; Li, Wenjiao; Zhu, Ping; Myatt, Leslie; Sun, Kang.

In: Biology of Reproduction, Vol. 78, No. 1, 01.2008, p. 193-197.

Research output: Contribution to journalArticle

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abstract = "Glucocorticoids (GCs) are well-known anti-inflammatory drugs inhibiting prostaglandin production. Paradoxically, GCs are reported to stimulate cytosolic phosphoplipase A2 group IVA (PLA2G4A) and prostaglandin-endoperoxide synthase 2 (PTGS2) expression in human amnion fibroblasts. This study was designed to examine the molecular mechanisms underlying glucocorticoid-induced PLA2G4A expression in human amnion fibroblasts. Our data showed that cortisol (0.01∼1 μM) increased PLA2G4A mRNA level in a dose-dependent manner in human amnion fibroblasts, which was blocked by glucocorticoid receptor antagonist RU486 (1 μM) as well as by the mRNA transcription inhibitor 5,6-dichlorobenzimidazole riboside (DRB; 75 μM). Concurrently, cortisol (0.01∼1 μM) decreased rather than increased proinflammatory cytokine mRNA levels, including interleukin 1 beta (IL1B), interleukin 6 (IL6), and tumor necrosis factor alpha (TNF), in a dose-dependent manner in human amnion fibroblasts. Chromatin immunoprecipitation assay revealed that glucocorticoid receptor was bound to PLA2G4A promoter in human amnion fibroblasts upon cortisol stimulation. This was confirmed by electrophoretic mobility shift assay showing that nuclear protein extracted from human amnion fibroblasts upon cortisol stimulation could bind the synthesized oligonucleotide sequence corresponding to PLA2G4A promoter region from -95 bp to -65 bp bearing the putative glucocorticoid response element This binding was super shifted by glucocorticoid receptor antibody. In conclusion, we demonstrated in this study that cortisol increased PLA2G4A mRNA level via GR-dependent ongoing transcription in human amnion fibroblasts by activating the binding of GR to PLA2G4A promoter directly, and this effect appeared unlikely to be secondary to the effect of cortisol on the expression of proinflammatory cytokines in human amnion fibroblasts.",
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