PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis

Thomas Simmen, Joseph Aslan, Anastassia D. Blagoveshchenskaya, Laurel Thomas, Lei Wan, Yang Xiang, Sylvain F. Feliciangeli, Chien Hui Hung, Colin M. Crump, Gary Thomas

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) and mitochondria form contacts that support communication between these two organelles, including synthesis and transfer of lipids, and the exchange of calcium, which regulates ER chaperones, mitochondrial ATP production, and apoptosis. Despite the fundamental roles for ER-mitochondria contacts, little is known about the molecules that regulate them. Here we report the identification of a multifunctional sorting protein, PACS-2, that integrates ER-mitochondria communication, ER homeostasis, and apoptosis. PACS-2 controls the apposition of mitochondria with the ER, as depletion of PACS-2 causes BAP31-dependent mitochondria fragmentation and uncoupling from the ER. PACS-2 also controls formation of ER lipid-synthesizing centers found on mitochondria-associated membranes and ER homeostasis. However, in response to apoptotic inducers, PACS-2 translocates Bid to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated Bid, the release of cytochrome c, and the activation of caspase-3, thereby causing cell death. Together, our results identify PACS-2 as a novel sorting protein that links the ER-mitochondria axis to ER homeostasis and the control of cell fate, and provide new insights into Bid action.

Original languageEnglish (US)
Pages (from-to)717-729
Number of pages13
JournalEMBO Journal
Volume24
Issue number4
DOIs
StatePublished - Feb 23 2005

Fingerprint

Picture archiving and communication systems
Mitochondria
Endoplasmic Reticulum
Apoptosis
Communication
Sorting
Homeostasis
Protein Transport
Lipids
Cell death
Cytochromes c
Caspase 3
Ion exchange
Adenosine Triphosphate
Chemical activation
Organelles
Calcium
Membranes
Molecules
Cell Death

Keywords

  • Apoptosis
  • Bid
  • Endoplasmic reticulum
  • Mitochondria
  • PACS-2

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Simmen, T., Aslan, J., Blagoveshchenskaya, A. D., Thomas, L., Wan, L., Xiang, Y., ... Thomas, G. (2005). PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. EMBO Journal, 24(4), 717-729. https://doi.org/10.1038/sj.emboj.7600559

PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. / Simmen, Thomas; Aslan, Joseph; Blagoveshchenskaya, Anastassia D.; Thomas, Laurel; Wan, Lei; Xiang, Yang; Feliciangeli, Sylvain F.; Hung, Chien Hui; Crump, Colin M.; Thomas, Gary.

In: EMBO Journal, Vol. 24, No. 4, 23.02.2005, p. 717-729.

Research output: Contribution to journalArticle

Simmen, T, Aslan, J, Blagoveshchenskaya, AD, Thomas, L, Wan, L, Xiang, Y, Feliciangeli, SF, Hung, CH, Crump, CM & Thomas, G 2005, 'PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis', EMBO Journal, vol. 24, no. 4, pp. 717-729. https://doi.org/10.1038/sj.emboj.7600559
Simmen, Thomas ; Aslan, Joseph ; Blagoveshchenskaya, Anastassia D. ; Thomas, Laurel ; Wan, Lei ; Xiang, Yang ; Feliciangeli, Sylvain F. ; Hung, Chien Hui ; Crump, Colin M. ; Thomas, Gary. / PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. In: EMBO Journal. 2005 ; Vol. 24, No. 4. pp. 717-729.
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