Overexpression of glia maturation factor in C6 cells promotes differentiation and activates superoxide dismutase

Ramon Lim, Asgar Zaheer, Jeff A. Kraakevik, Christine J. Darby, Larry W. Oberley

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In order to evaluate the intracellular function of glia maturation factor (GMF), we overexpressed GMF in C6 rat glioma cells using two methods: stable transfection using the pcDNA3 plasmid, and transient transfection using replication-defective human adenovirus. With both methods, C6 cells transfected with GMF and overexpressing the protein exhibit a lower saturation density in culture compared to non-transfected or vector alone controls. Transfected cells also exhibit morphological differentiation as shown by the outgrowth of cell processes. When inoculated into nude mice, transfected cells are less tumorigenic than controls, and express the mature astrocytic marker glial fibrillary acidic protein. In tissue culture, transfected cells show a 3.5-fold increase in CuZn-dependent superoxide dismutase (CuZnSOD) activity. Western blot analysis reveals a 3.5-fold increase in CuZnSOD protein, suggesting an induction of the enzyme. In view of recent findings that reactive oxygen species (ROS) and the antioxidant enzymes are intricately involved in key physiologic processes such as proliferation, differentiation and apoptosis, the study raises the possibility that CuZnSOD may be a mediator of GMF function.

Original languageEnglish (US)
Pages (from-to)1445-1451
Number of pages7
JournalNeurochemical Research
Volume23
Issue number11
DOIs
StatePublished - Nov 14 1998

Keywords

  • Antioxidant enzymes
  • Glia
  • Glioma
  • Maturation
  • Oxidative stress
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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