Oral microbiota species in acute apical endodontic abscesses

Noelle George, Erin Flamiatos, Kellie Kawasaki, Namgu Kim, Charles Carriere, Brian Phan, Raphael Joseph, Shay Strauss, Richie Kohli, Dongseok Choi, John (Craig) Baumgartner, Christine Sedgley, Tom Maier, Curtis Machida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N=18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNAwas then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.

Original languageEnglish (US)
Article number30989
JournalJournal of Oral Microbiology
Volume8
Issue number1
DOIs
StatePublished - 2016

Fingerprint

Endodontics
Microbiota
Abscess
Porphyromonas endodontalis
Megasphaera
Clone Cells
Streptococcus
Demography
Prevotella
Fusobacterium nucleatum
Veillonellaceae
Streptococcus constellatus
Treponema
School Dentistry
Actinomyces
Opportunistic Infections
rRNA Genes
Polymerase Chain Reaction
DNA
Health

Keywords

  • Anaerobic oral microorganisms
  • Endodontic abscesses
  • Fusobacterium nucleatum
  • Human oral microbial identification microarrays
  • Oral microbiota
  • Streptococcus cristatus

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

George, N., Flamiatos, E., Kawasaki, K., Kim, N., Carriere, C., Phan, B., ... Machida, C. (2016). Oral microbiota species in acute apical endodontic abscesses. Journal of Oral Microbiology, 8(1), [30989]. https://doi.org/10.3402/jom.v8.30989

Oral microbiota species in acute apical endodontic abscesses. / George, Noelle; Flamiatos, Erin; Kawasaki, Kellie; Kim, Namgu; Carriere, Charles; Phan, Brian; Joseph, Raphael; Strauss, Shay; Kohli, Richie; Choi, Dongseok; Baumgartner, John (Craig); Sedgley, Christine; Maier, Tom; Machida, Curtis.

In: Journal of Oral Microbiology, Vol. 8, No. 1, 30989, 2016.

Research output: Contribution to journalArticle

George N, Flamiatos E, Kawasaki K, Kim N, Carriere C, Phan B et al. Oral microbiota species in acute apical endodontic abscesses. Journal of Oral Microbiology. 2016;8(1). 30989. https://doi.org/10.3402/jom.v8.30989
George, Noelle ; Flamiatos, Erin ; Kawasaki, Kellie ; Kim, Namgu ; Carriere, Charles ; Phan, Brian ; Joseph, Raphael ; Strauss, Shay ; Kohli, Richie ; Choi, Dongseok ; Baumgartner, John (Craig) ; Sedgley, Christine ; Maier, Tom ; Machida, Curtis. / Oral microbiota species in acute apical endodontic abscesses. In: Journal of Oral Microbiology. 2016 ; Vol. 8, No. 1.
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AU - Joseph, Raphael

AU - Strauss, Shay

AU - Kohli, Richie

AU - Choi, Dongseok

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N2 - Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N=18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNAwas then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.

AB - Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N=18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNAwas then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.

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KW - Fusobacterium nucleatum

KW - Human oral microbial identification microarrays

KW - Oral microbiota

KW - Streptococcus cristatus

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