Optical mapping in a new guinea pig model of ventricular tachycardia reveals mechanisms for multiple wavelengths in a single reentrant circuit

Steven D. Girouard, Joseph M. Pastore, Kenneth R. Laurita, Kenton Gregory, David S. Rosenbaum

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Background Although the relationship between cardiac wavelength (λ) and path length importantly determines the stability of reentrant arrhythmias, the physiological determinants of λ are poorly understood. To investigate the cellular mechanisms that control λ during reentry, we developed an experimental system for continuously monitoring λ within a reentrant circuit with the use of voltage-sensitive dyes and a new guinea pig model of ventricular tachycardia (VT). Methods and Results: Action potentials were recorded simultaneously from 128 ventricular sites in Langendorff-perfused hearts (n=15) in which propagation was confined to a two-dimensional rim of epicardium by an endocardial cryoablating procedure. The reentrant path was precisely controlled by creating an epicardial obstacle (2x10 mm) with an argon laser. To control for fiber orientation and rate-dependent membrane properties, λ during reentry was compared with λ during plane wave propagation transverse and longitudinal to cardiac fibers at a stimulus cycle length (CL) comparable to the VT CL. Reentrant VT (CL=97.0±6.2 ms) was reproducibly induced by programmed stimulation in 93% of preparations. λ varied considerably within the reentrant circuit (range, 10.6 to 22.5 mm), because of heterogeneities of conduction rather than action potential duration. λ was significantly shorter during reentrant propagation (ie, with pivoting) parallel to fibers (10.6±4.2 mm) compared with plane wave propagation (ie, without pivoting) parallel to fibers (32.8±6.5 mm, P

Original languageEnglish (US)
Pages (from-to)603-613
Number of pages11
JournalCirculation
Volume93
Issue number3
StatePublished - Feb 1 1996
Externally publishedYes

Fingerprint

New Guinea
Ventricular Tachycardia
Guinea Pigs
Action Potentials
Argon
Pericardium
Cardiac Arrhythmias
Lasers
Coloring Agents
Membranes

Keywords

  • action potentials
  • electrophysiology
  • mapping
  • reentry
  • tachycardia

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Optical mapping in a new guinea pig model of ventricular tachycardia reveals mechanisms for multiple wavelengths in a single reentrant circuit. / Girouard, Steven D.; Pastore, Joseph M.; Laurita, Kenneth R.; Gregory, Kenton; Rosenbaum, David S.

In: Circulation, Vol. 93, No. 3, 01.02.1996, p. 603-613.

Research output: Contribution to journalArticle

Girouard, Steven D. ; Pastore, Joseph M. ; Laurita, Kenneth R. ; Gregory, Kenton ; Rosenbaum, David S. / Optical mapping in a new guinea pig model of ventricular tachycardia reveals mechanisms for multiple wavelengths in a single reentrant circuit. In: Circulation. 1996 ; Vol. 93, No. 3. pp. 603-613.
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