Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides

Matthijs Kol, Ben Williams, Henry Toombs-Ruane, Henri G. Franquelim, Sergei Korneev, Christian Schroeer, Petra Schwille, Dirk Trauner, Joost Cm Holthuis, James Frank

Research output: Contribution to journalArticle

Abstract

Ceramides are central intermediates of sphingolipid metabolism that also function as potent messengers in stress signaling and apoptosis. Progress in understanding how ceramides execute their biological roles is hampered by a lack of methods to manipulate their cellular levels and metabolic fate with appropriate spatiotemporal precision. Here, we report on clickable, azobenzene-containing ceramides, caCers, as photoswitchable metabolic substrates to exert optical control over sphingolipid production in cells. Combining atomic force microscopy on model bilayers with metabolic tracing studies in cells, we demonstrate that light-induced alterations in the lateral packing of caCers lead to marked differences in their metabolic conversion by sphingomyelin synthase and glucosylceramide synthase. These changes in metabolic rates are instant and reversible over several cycles of photoswitching. Our findings disclose new opportunities to probe the causal roles of ceramides and their metabolic derivatives in a wide array of sphingolipid-dependent cellular processes with the spatiotemporal precision of light.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Feb 5 2019

Fingerprint

Sphingolipids
Ceramides
Biosynthesis
ceramide glucosyltransferase
Light
Atomic Force Microscopy
Metabolism
Atomic force microscopy
Apoptosis
Derivatives
Substrates

Keywords

  • atomic force microscopy
  • biochemistry
  • ceramide
  • chemical biology
  • glucosylceramide
  • HeLa cells
  • photopharmacology
  • sphingomyelin synthase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kol, M., Williams, B., Toombs-Ruane, H., Franquelim, H. G., Korneev, S., Schroeer, C., ... Frank, J. (2019). Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides. eLife, 8. https://doi.org/10.7554/eLife.43230

Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides. / Kol, Matthijs; Williams, Ben; Toombs-Ruane, Henry; Franquelim, Henri G.; Korneev, Sergei; Schroeer, Christian; Schwille, Petra; Trauner, Dirk; Holthuis, Joost Cm; Frank, James.

In: eLife, Vol. 8, 05.02.2019.

Research output: Contribution to journalArticle

Kol, M, Williams, B, Toombs-Ruane, H, Franquelim, HG, Korneev, S, Schroeer, C, Schwille, P, Trauner, D, Holthuis, JC & Frank, J 2019, 'Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides', eLife, vol. 8. https://doi.org/10.7554/eLife.43230
Kol M, Williams B, Toombs-Ruane H, Franquelim HG, Korneev S, Schroeer C et al. Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides. eLife. 2019 Feb 5;8. https://doi.org/10.7554/eLife.43230
Kol, Matthijs ; Williams, Ben ; Toombs-Ruane, Henry ; Franquelim, Henri G. ; Korneev, Sergei ; Schroeer, Christian ; Schwille, Petra ; Trauner, Dirk ; Holthuis, Joost Cm ; Frank, James. / Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides. In: eLife. 2019 ; Vol. 8.
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