Opioids hyperpolarize β-endorphin neurons via μ-receptor activation of a potassium conductance

Martin Kelly, Michael D. Loose, Oline Ronnekleiv

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Intracellular recordings were made from hypothalamic arcuate (ARC) neurons with biocytin-filled electrodes under current- and voltage-clamp in slices prepared from ovariectomized guinea pigs which were pretreated with estradiol. Forty-three neurons were identified after linking the intracellular biocytin with streptavidin-FITC and subsequently were examined for β-endorphin immunoreactivity. Ten of these neurons were immunoreactive for β-endorphin. β-Endorphin neurons displayed the following passive membrane properties: RMP:-56 ± 2 mV; Rin: 439 ± 66 MG; τ: 17.5 ± 2.4 ms; and often fired spontaneously (5.9 ± 2.2 Hz). These membrane characteristics were not different from identified neurons in the ARC that were not immunoreactive for ᵝ endorphin. β-Endorphin neurons exhibited instantaneous inward rectification and time-dependent rectification. The μ-opioid agonist Tyr-D-Ala-Gly-MePhe-Gly-oI (DAGO) decreased spontaneous firing, induced membrane hyperpoiarization (12 ± 2 mV; range 6–22 m V) and decreased the Rin (38 ± 4%) of the β-endorphin neurons. These effects of DAGO were blocked by the opioid antagonist naloxone (I pAT) and were not blocked by 1 μMTTX. DAGO-responsive cells were unaffected by either k- or δ-receptor opioid agonists. These results indicate that p-receptors may be autoreceptors on ARC β-endorphin neurons and that activation of opioid p-receptors hyperpolarizes β-endorphin neurons via an increase in K+conductance. Therefore, opioid peptides may modulate opioid tone through an ‘ultra-short loop’ feedback control mechanism.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalNeuroendocrinology
Volume52
Issue number3
DOIs
StatePublished - 1990

Fingerprint

Endorphins
Opioid Analgesics
Potassium
Neurons
alanylglycine
Opioid Receptors
Membranes
Autoreceptors
Streptavidin
Opioid Peptides
Narcotic Antagonists
Fluorescein-5-isothiocyanate
Naloxone
Estradiol
Electrodes
Guinea Pigs

Keywords

  • Arcuate
  • Autoreceptor
  • Hyperpolarization
  • Hypothalamus
  • K<sup>+</sup>conductance
  • Voltage clamp
  • β-Endorphin
  • μ-Opioid receptor

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Opioids hyperpolarize β-endorphin neurons via μ-receptor activation of a potassium conductance. / Kelly, Martin; Loose, Michael D.; Ronnekleiv, Oline.

In: Neuroendocrinology, Vol. 52, No. 3, 1990, p. 268-275.

Research output: Contribution to journalArticle

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abstract = "Intracellular recordings were made from hypothalamic arcuate (ARC) neurons with biocytin-filled electrodes under current- and voltage-clamp in slices prepared from ovariectomized guinea pigs which were pretreated with estradiol. Forty-three neurons were identified after linking the intracellular biocytin with streptavidin-FITC and subsequently were examined for β-endorphin immunoreactivity. Ten of these neurons were immunoreactive for β-endorphin. β-Endorphin neurons displayed the following passive membrane properties: RMP:-56 ± 2 mV; Rin: 439 ± 66 MG; τ: 17.5 ± 2.4 ms; and often fired spontaneously (5.9 ± 2.2 Hz). These membrane characteristics were not different from identified neurons in the ARC that were not immunoreactive for ᵝ endorphin. β-Endorphin neurons exhibited instantaneous inward rectification and time-dependent rectification. The μ-opioid agonist Tyr-D-Ala-Gly-MePhe-Gly-oI (DAGO) decreased spontaneous firing, induced membrane hyperpoiarization (12 ± 2 mV; range 6–22 m V) and decreased the Rin (38 ± 4{\%}) of the β-endorphin neurons. These effects of DAGO were blocked by the opioid antagonist naloxone (I pAT) and were not blocked by 1 μMTTX. DAGO-responsive cells were unaffected by either k- or δ-receptor opioid agonists. These results indicate that p-receptors may be autoreceptors on ARC β-endorphin neurons and that activation of opioid p-receptors hyperpolarizes β-endorphin neurons via an increase in K+conductance. Therefore, opioid peptides may modulate opioid tone through an ‘ultra-short loop’ feedback control mechanism.",
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