Abstract
Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 227-232 |
| Number of pages | 6 |
| Journal | Journal of Affective Disorders |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
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Keywords
- Affective disorders
- Diphenylhexatriene
- Fluorescence polarization
- Membrane order
- Membranes
- Psychoses
ASJC Scopus subject areas
- Psychiatry and Mental health
- Behavioral Neuroscience
- Biological Psychiatry
- Neurology
- Psychology(all)
Cite this
On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study. / Hitzemann, Robert; Hirschowitz, Jack; Garver, David L.
In: Journal of Affective Disorders, Vol. 10, No. 3, 1986, p. 227-232.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study
AU - Hitzemann, Robert
AU - Hirschowitz, Jack
AU - Garver, David L.
PY - 1986
Y1 - 1986
N2 - Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.
AB - Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.
KW - Affective disorders
KW - Diphenylhexatriene
KW - Fluorescence polarization
KW - Membrane order
KW - Membranes
KW - Psychoses
UR - http://www.scopus.com/inward/record.url?scp=0022501155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022501155&partnerID=8YFLogxK
U2 - 10.1016/0165-0327(86)90009-1
DO - 10.1016/0165-0327(86)90009-1
M3 - Article
C2 - 2943777
AN - SCOPUS:0022501155
VL - 10
SP - 227
EP - 232
JO - Journal of Affective Disorders
JF - Journal of Affective Disorders
SN - 0165-0327
IS - 3
ER -