On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study

Robert Hitzemann, Jack Hirschowitz, David L. Garver

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalJournal of Affective Disorders
Volume10
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Psychotic Affective Disorders
Fluorescence Polarization
Erythrocyte Membrane
Mood Disorders
Cell Membrane
Membranes
Diagnostic and Statistical Manual of Mental Disorders
Psychotic Disorders
Phosphatidylcholines
Entropy
Age Groups
Control Groups

Keywords

  • Affective disorders
  • Diphenylhexatriene
  • Fluorescence polarization
  • Membrane order
  • Membranes
  • Psychoses

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Behavioral Neuroscience
  • Biological Psychiatry
  • Neurology
  • Psychology(all)

Cite this

On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study. / Hitzemann, Robert; Hirschowitz, Jack; Garver, David L.

In: Journal of Affective Disorders, Vol. 10, No. 3, 1986, p. 227-232.

Research output: Contribution to journalArticle

@article{80558cbe13bf4e208412ee7e43285e28,
title = "On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study",
abstract = "Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.",
keywords = "Affective disorders, Diphenylhexatriene, Fluorescence polarization, Membrane order, Membranes, Psychoses",
author = "Robert Hitzemann and Jack Hirschowitz and Garver, {David L.}",
year = "1986",
doi = "10.1016/0165-0327(86)90009-1",
language = "English (US)",
volume = "10",
pages = "227--232",
journal = "Journal of Affective Disorders",
issn = "0165-0327",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study

AU - Hitzemann, Robert

AU - Hirschowitz, Jack

AU - Garver, David L.

PY - 1986

Y1 - 1986

N2 - Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.

AB - Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P <0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.

KW - Affective disorders

KW - Diphenylhexatriene

KW - Fluorescence polarization

KW - Membrane order

KW - Membranes

KW - Psychoses

UR - http://www.scopus.com/inward/record.url?scp=0022501155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022501155&partnerID=8YFLogxK

U2 - 10.1016/0165-0327(86)90009-1

DO - 10.1016/0165-0327(86)90009-1

M3 - Article

VL - 10

SP - 227

EP - 232

JO - Journal of Affective Disorders

JF - Journal of Affective Disorders

SN - 0165-0327

IS - 3

ER -