On the physical properties of red cell ghost membranes in the affective disorders and psychoses. A fluorescence polarization study

Robert J. Hitzemann, Jack Hirschowitz, David L. Garver

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Membrane order was measured in the erythrocyte ghost membranes of DSM-III schizophreniform disorder (SF), DSM-III schizophrenic (SCZ) and DSM-III manic (bipolar) (M) patients and a group of age- and sex-matched controls. Fluorescence polarization with the probe 1,6-diphenyl1-1,3,5-hexatriene was used to determine the steady-state fluorescence anisotropy (rs). The SF group showed a significant increase in rs (Δrs = 0.037) from the control group. Although the means were not significantly different, 3 of 8 Ms and 5 of 8 SCZs also had rs values > the highest control value. Thermotropic behavior of the membranes was evaluated over the range of 40 to 20°C. No difference among groups in membrane enthalpy was detected. Thus, the differences in rs appear to be associated with differences in entropy. Phosphatidylcholine (PC) levels, which were known to be abnormal in these patients, were compared with the rs values. A significant (P < 0.001, r= -0.63) linear correlation between rs and membrane PC levels was observed. Overall these data further support the view that unusual membrane biophysical factors may occur with high frequency in the psychoses and affective disorders.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalJournal of Affective Disorders
Volume10
Issue number3
DOIs
StatePublished - Jan 1 1986

Keywords

  • Affective disorders
  • Diphenylhexatriene
  • Fluorescence polarization
  • Membrane order
  • Membranes
  • Psychoses

ASJC Scopus subject areas

  • Clinical Psychology
  • Psychiatry and Mental health

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