Observation of transient perfusion defects in a mouse heart using contrast ultrasound

John A. Hossack, Jonathan C. Christensen, Brent A. French, Zequan Yang, Jiri Sklenar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

There is a significant interest in noninvasive approaches for tracking anatomical and physiological changes resulting from myocardial infarction in the mouse heart. We assessed perfusion before, during and after an induced closed chest coronary ischemia. Ultrasound contrast agent, in a saline suspension, was injected via jugular cannulation in a bolus. This gave rise to contrast agent related echogenic enhancement in the right ventricle (RV), left ventricle (LV) and finally the myocardium (muscle tissue of the heart) in acquired short axis views. Image sequences were transferred from the scanner to a computer for analysis. Regions of interest were manually defined in septal and anterior segments of the myocardium. During the ischemic event, which degraded perfusion in the anterior segment, mean video intensity in the affected segment was reduced by approximately one half. However, following the removal of ischemia, hyperemia (enhanced blood flow) was observed. Specifically, the mean video intensity in the affected segment was increased by approximately 50% over the original baseline level prior to ischemia. In this way it may be possible to quantify myocardial blood flow in perfusion deficient regions of a mouse heart using computer automated analysis of contrast image sequences.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE Ultrasonics Symposium
EditorsD.E. Yuhas, S.C. Schneider
Pages641-644
Number of pages4
Volume1
StatePublished - 2003
Externally publishedYes
Event2003 IEEE Ultrasonics Symposium - Proceedings - Honolulu, HI, United States
Duration: Oct 5 2003Oct 8 2003

Other

Other2003 IEEE Ultrasonics Symposium - Proceedings
CountryUnited States
CityHonolulu, HI
Period10/5/0310/8/03

Fingerprint

Ultrasonics
Defects
Blood
Muscle
Tissue

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hossack, J. A., Christensen, J. C., French, B. A., Yang, Z., & Sklenar, J. (2003). Observation of transient perfusion defects in a mouse heart using contrast ultrasound. In D. E. Yuhas, & S. C. Schneider (Eds.), Proceedings of the IEEE Ultrasonics Symposium (Vol. 1, pp. 641-644)

Observation of transient perfusion defects in a mouse heart using contrast ultrasound. / Hossack, John A.; Christensen, Jonathan C.; French, Brent A.; Yang, Zequan; Sklenar, Jiri.

Proceedings of the IEEE Ultrasonics Symposium. ed. / D.E. Yuhas; S.C. Schneider. Vol. 1 2003. p. 641-644.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hossack, JA, Christensen, JC, French, BA, Yang, Z & Sklenar, J 2003, Observation of transient perfusion defects in a mouse heart using contrast ultrasound. in DE Yuhas & SC Schneider (eds), Proceedings of the IEEE Ultrasonics Symposium. vol. 1, pp. 641-644, 2003 IEEE Ultrasonics Symposium - Proceedings, Honolulu, HI, United States, 10/5/03.
Hossack JA, Christensen JC, French BA, Yang Z, Sklenar J. Observation of transient perfusion defects in a mouse heart using contrast ultrasound. In Yuhas DE, Schneider SC, editors, Proceedings of the IEEE Ultrasonics Symposium. Vol. 1. 2003. p. 641-644
Hossack, John A. ; Christensen, Jonathan C. ; French, Brent A. ; Yang, Zequan ; Sklenar, Jiri. / Observation of transient perfusion defects in a mouse heart using contrast ultrasound. Proceedings of the IEEE Ultrasonics Symposium. editor / D.E. Yuhas ; S.C. Schneider. Vol. 1 2003. pp. 641-644
@inproceedings{9e679149242b4d31b65f0286fdff2d28,
title = "Observation of transient perfusion defects in a mouse heart using contrast ultrasound",
abstract = "There is a significant interest in noninvasive approaches for tracking anatomical and physiological changes resulting from myocardial infarction in the mouse heart. We assessed perfusion before, during and after an induced closed chest coronary ischemia. Ultrasound contrast agent, in a saline suspension, was injected via jugular cannulation in a bolus. This gave rise to contrast agent related echogenic enhancement in the right ventricle (RV), left ventricle (LV) and finally the myocardium (muscle tissue of the heart) in acquired short axis views. Image sequences were transferred from the scanner to a computer for analysis. Regions of interest were manually defined in septal and anterior segments of the myocardium. During the ischemic event, which degraded perfusion in the anterior segment, mean video intensity in the affected segment was reduced by approximately one half. However, following the removal of ischemia, hyperemia (enhanced blood flow) was observed. Specifically, the mean video intensity in the affected segment was increased by approximately 50{\%} over the original baseline level prior to ischemia. In this way it may be possible to quantify myocardial blood flow in perfusion deficient regions of a mouse heart using computer automated analysis of contrast image sequences.",
author = "Hossack, {John A.} and Christensen, {Jonathan C.} and French, {Brent A.} and Zequan Yang and Jiri Sklenar",
year = "2003",
language = "English (US)",
volume = "1",
pages = "641--644",
editor = "D.E. Yuhas and S.C. Schneider",
booktitle = "Proceedings of the IEEE Ultrasonics Symposium",

}

TY - GEN

T1 - Observation of transient perfusion defects in a mouse heart using contrast ultrasound

AU - Hossack, John A.

AU - Christensen, Jonathan C.

AU - French, Brent A.

AU - Yang, Zequan

AU - Sklenar, Jiri

PY - 2003

Y1 - 2003

N2 - There is a significant interest in noninvasive approaches for tracking anatomical and physiological changes resulting from myocardial infarction in the mouse heart. We assessed perfusion before, during and after an induced closed chest coronary ischemia. Ultrasound contrast agent, in a saline suspension, was injected via jugular cannulation in a bolus. This gave rise to contrast agent related echogenic enhancement in the right ventricle (RV), left ventricle (LV) and finally the myocardium (muscle tissue of the heart) in acquired short axis views. Image sequences were transferred from the scanner to a computer for analysis. Regions of interest were manually defined in septal and anterior segments of the myocardium. During the ischemic event, which degraded perfusion in the anterior segment, mean video intensity in the affected segment was reduced by approximately one half. However, following the removal of ischemia, hyperemia (enhanced blood flow) was observed. Specifically, the mean video intensity in the affected segment was increased by approximately 50% over the original baseline level prior to ischemia. In this way it may be possible to quantify myocardial blood flow in perfusion deficient regions of a mouse heart using computer automated analysis of contrast image sequences.

AB - There is a significant interest in noninvasive approaches for tracking anatomical and physiological changes resulting from myocardial infarction in the mouse heart. We assessed perfusion before, during and after an induced closed chest coronary ischemia. Ultrasound contrast agent, in a saline suspension, was injected via jugular cannulation in a bolus. This gave rise to contrast agent related echogenic enhancement in the right ventricle (RV), left ventricle (LV) and finally the myocardium (muscle tissue of the heart) in acquired short axis views. Image sequences were transferred from the scanner to a computer for analysis. Regions of interest were manually defined in septal and anterior segments of the myocardium. During the ischemic event, which degraded perfusion in the anterior segment, mean video intensity in the affected segment was reduced by approximately one half. However, following the removal of ischemia, hyperemia (enhanced blood flow) was observed. Specifically, the mean video intensity in the affected segment was increased by approximately 50% over the original baseline level prior to ischemia. In this way it may be possible to quantify myocardial blood flow in perfusion deficient regions of a mouse heart using computer automated analysis of contrast image sequences.

UR - http://www.scopus.com/inward/record.url?scp=4143100436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143100436&partnerID=8YFLogxK

M3 - Conference contribution

VL - 1

SP - 641

EP - 644

BT - Proceedings of the IEEE Ultrasonics Symposium

A2 - Yuhas, D.E.

A2 - Schneider, S.C.

ER -