Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I)

David Grandy, Richard Leduc, Haripriya Makam, Thomas Flanagan, Emanuel J. Diliberto, Olivier Civelli, O. Humberto Viveros

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745) was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain.

    Original languageEnglish (US)
    Pages (from-to)185-192
    Number of pages8
    JournalCellular and Molecular Neurobiology
    Volume12
    Issue number2
    DOIs
    StatePublished - Apr 1992

    Fingerprint

    Chromogranin B
    Adrenal Medulla
    Amino Acid Sequence
    Nucleotides
    Amino Acids
    Basic Amino Acids
    Peptides
    Oligonucleotide Probes
    Chromaffin Granules
    Protein Sorting Signals
    Processing
    Cysteine
    Tyrosine
    Rats
    Proteins
    Complementary DNA
    BAM 1745

    Keywords

    • chromogranin B
    • nucleotide sequence
    • pyroglutamyl peptide
    • secretogranin I
    • sequence comparison

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Genetics
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    Grandy, D., Leduc, R., Makam, H., Flanagan, T., Diliberto, E. J., Civelli, O., & Viveros, O. H. (1992). Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I). Cellular and Molecular Neurobiology, 12(2), 185-192. https://doi.org/10.1007/BF00713372

    Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I). / Grandy, David; Leduc, Richard; Makam, Haripriya; Flanagan, Thomas; Diliberto, Emanuel J.; Civelli, Olivier; Viveros, O. Humberto.

    In: Cellular and Molecular Neurobiology, Vol. 12, No. 2, 04.1992, p. 185-192.

    Research output: Contribution to journalArticle

    Grandy, D, Leduc, R, Makam, H, Flanagan, T, Diliberto, EJ, Civelli, O & Viveros, OH 1992, 'Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I)', Cellular and Molecular Neurobiology, vol. 12, no. 2, pp. 185-192. https://doi.org/10.1007/BF00713372
    Grandy, David ; Leduc, Richard ; Makam, Haripriya ; Flanagan, Thomas ; Diliberto, Emanuel J. ; Civelli, Olivier ; Viveros, O. Humberto. / Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I). In: Cellular and Molecular Neurobiology. 1992 ; Vol. 12, No. 2. pp. 185-192.
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