TY - JOUR
T1 - Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I)
AU - Grandy, David K.
AU - Leduc, Richard
AU - Makam, Haripriya
AU - Flanagan, Thomas
AU - Diliberto, Emanuel J.
AU - Civelli, Olivier
AU - Viveros, O. Humberto
PY - 1992/4
Y1 - 1992/4
N2 - 1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745) was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain.
AB - 1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745) was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain.
KW - chromogranin B
KW - nucleotide sequence
KW - pyroglutamyl peptide
KW - secretogranin I
KW - sequence comparison
UR - http://www.scopus.com/inward/record.url?scp=0026511789&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026511789&partnerID=8YFLogxK
U2 - 10.1007/BF00713372
DO - 10.1007/BF00713372
M3 - Article
C2 - 1350945
AN - SCOPUS:0026511789
SN - 0272-4340
VL - 12
SP - 185
EP - 192
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
IS - 2
ER -