Nucleolar organizer regions in determining malignancy of pigmented conjunctival lesions

M. A. Saornil, D. M. Marcus, D. Doepner, G. Apolone, V. Torre, Daniel Albert

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We assessed the usefulness of silver staining of nucleolar organizer regions in the diagnosis of pigmented conjunctival tumors. Fifty-one biopsy specimens were silver stained to identify the nucleolar organizer regions. Nineteen nevi without atypia, three nevi with atypia, eight primary acquired melanosis lesions, and 14 melanomas were studied. In each specimen, silver staining of the nucleolar organizer regions was counted in 100 cells to yield an average of the silver staining of the nucleolar organizer region count. The mean silver staining of the nucleolar organizer region counts per cell was correlated with the degree of malignancy of pigmented conjunctival lesions as follows: nevi, 3.0; primary acquired melanosis, 3.2; nevi with atypia, 3.9; primary acquired melanosis with atypia, 5.0; and melanoma, 5.7 (Spearman correlation [rS] = .83, P = .0001; analysis of variance [ANOVA] F test = 20.9, P = .0001). A cutoff value of 4.0 (mean silver staining of nucleolar organizer regions per cell) will differentiate melanoma and primary acquired melanosis with atypia from other lesions (sensitivity, 100%; specificity, 96%). The silver staining of nucleolar organizer regions is a useful adjunct in determining the malignancy of pigmented conjunctival tumors.

Original languageEnglish (US)
Pages (from-to)800-805
Number of pages6
JournalAmerican journal of ophthalmology
Volume115
Issue number6
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Nucleolus Organizer Region
Silver Staining
Melanosis
Nevus
Neoplasms
Melanoma
Silver
Analysis of Variance
Cell Count
Biopsy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nucleolar organizer regions in determining malignancy of pigmented conjunctival lesions. / Saornil, M. A.; Marcus, D. M.; Doepner, D.; Apolone, G.; Torre, V.; Albert, Daniel.

In: American journal of ophthalmology, Vol. 115, No. 6, 01.01.1993, p. 800-805.

Research output: Contribution to journalArticle

Saornil, M. A. ; Marcus, D. M. ; Doepner, D. ; Apolone, G. ; Torre, V. ; Albert, Daniel. / Nucleolar organizer regions in determining malignancy of pigmented conjunctival lesions. In: American journal of ophthalmology. 1993 ; Vol. 115, No. 6. pp. 800-805.
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