TY - JOUR
T1 - Nucleolar organizer regions in determining malignancy of pigmented conjunctival lesions
AU - Saornil, M. A.
AU - Marcus, D. M.
AU - Doepner, D.
AU - Apolone, G.
AU - Torre, V.
AU - Albert, D. M.
N1 - Funding Information:
From the David G. Cogan Eye Pathology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts (Drs. Saornil, Marcus, Doepner, and Albert); and the Istituto di Richerche Farmacologische Mario Negri, Laboratorio de Epidermologia Clinica, Milan, Italy (Dr. Apolone and Torre). This study was supported by grant EY01917 from the National Institutes of Health (Dr. Albert).
PY - 1993
Y1 - 1993
N2 - We assessed the usefulness of silver staining of nucleolar organizer regions in the diagnosis of pigmented conjunctival tumors. Fifty-one biopsy specimens were silver stained to identify the nucleolar organizer regions. Nineteen nevi without atypia, three nevi with atypia, eight primary acquired melanosis lesions, and 14 melanomas were studied. In each specimen, silver staining of the nucleolar organizer regions was counted in 100 cells to yield an average of the silver staining of the nucleolar organizer region count. The mean silver staining of the nucleolar organizer region counts per cell was correlated with the degree of malignancy of pigmented conjunctival lesions as follows: nevi, 3.0; primary acquired melanosis, 3.2; nevi with atypia, 3.9; primary acquired melanosis with atypia, 5.0; and melanoma, 5.7 (Spearman correlation [rS] = .83, P = .0001; analysis of variance [ANOVA] F test = 20.9, P = .0001). A cutoff value of 4.0 (mean silver staining of nucleolar organizer regions per cell) will differentiate melanoma and primary acquired melanosis with atypia from other lesions (sensitivity, 100%; specificity, 96%). The silver staining of nucleolar organizer regions is a useful adjunct in determining the malignancy of pigmented conjunctival tumors.
AB - We assessed the usefulness of silver staining of nucleolar organizer regions in the diagnosis of pigmented conjunctival tumors. Fifty-one biopsy specimens were silver stained to identify the nucleolar organizer regions. Nineteen nevi without atypia, three nevi with atypia, eight primary acquired melanosis lesions, and 14 melanomas were studied. In each specimen, silver staining of the nucleolar organizer regions was counted in 100 cells to yield an average of the silver staining of the nucleolar organizer region count. The mean silver staining of the nucleolar organizer region counts per cell was correlated with the degree of malignancy of pigmented conjunctival lesions as follows: nevi, 3.0; primary acquired melanosis, 3.2; nevi with atypia, 3.9; primary acquired melanosis with atypia, 5.0; and melanoma, 5.7 (Spearman correlation [rS] = .83, P = .0001; analysis of variance [ANOVA] F test = 20.9, P = .0001). A cutoff value of 4.0 (mean silver staining of nucleolar organizer regions per cell) will differentiate melanoma and primary acquired melanosis with atypia from other lesions (sensitivity, 100%; specificity, 96%). The silver staining of nucleolar organizer regions is a useful adjunct in determining the malignancy of pigmented conjunctival tumors.
UR - http://www.scopus.com/inward/record.url?scp=0027161478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027161478&partnerID=8YFLogxK
U2 - 10.1016/S0002-9394(14)73651-7
DO - 10.1016/S0002-9394(14)73651-7
M3 - Article
C2 - 8506916
AN - SCOPUS:0027161478
SN - 0002-9394
VL - 115
SP - 800
EP - 805
JO - American journal of ophthalmology
JF - American journal of ophthalmology
IS - 6
ER -