Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma

Suzana A. Kahn, Xin Wang, Ryan T. Nitta, Sharareh Gholamin, Johanna Theruvath, Gregor Hutter, Tej D. Azad, Lina Wadi, Sara Bolin, Vijay Ramaswamy, Rogelio Esparza, Kun Wei Liu, Michael Edwards, Fredrik J. Swartling, Debashis Sahoo, Gordon Li, Robert J. Wechsler-Reya, Jüri Reimand, Yoon-Jae Cho, Michael D. Taylor & 3 others Irving L. Weissman, Siddhartha S. Mitra, Samuel H. Cheshier

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Medulloblastoma is the most common malignant brain tumor of childhood. Group 3 medulloblastoma, the most aggressive molecular subtype, frequently disseminates through the leptomeningeal cerebral spinal fluid (CSF) spaces in the brain and spinal cord. The mechanism of dissemination through the CSF remains poorly understood, and the molecular pathways involved in medulloblastoma metastasis and self-renewal are largely unknown. Here we show that NOTCH1 signaling pathway regulates both the initiation of metastasis and the self-renewal of medulloblastoma. We identify a mechanism in which NOTCH1 activates BMI1 through the activation of TWIST1. NOTCH1 expression and activity are directly related to medulloblastoma metastasis and decreased survival rate of tumor-bearing mice. Finally, medulloblastoma-bearing mice intrathecally treated with anti-NRR1, a NOTCH1 blocking antibody, present lower frequency of spinal metastasis and higher survival rate. These findings identify NOTCH1 as a pivotal driver of Group 3 medulloblastoma metastasis and self-renewal, supporting the development of therapies targeting this pathway.

Original languageEnglish (US)
Article number4121
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Fingerprint

Bearings (structural)
Medulloblastoma
metastasis
Tumors
Brain
Neoplasm Metastasis
Blocking Antibodies
Fluids
Chemical activation
brain
mice
tumors
spinal cord
fluids
antibodies
therapy
activation
Brain Neoplasms
low frequencies
Spinal Cord

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Kahn, S. A., Wang, X., Nitta, R. T., Gholamin, S., Theruvath, J., Hutter, G., ... Cheshier, S. H. (2018). Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma. Nature communications, 9(1), [4121]. https://doi.org/10.1038/s41467-018-06564-9

Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma. / Kahn, Suzana A.; Wang, Xin; Nitta, Ryan T.; Gholamin, Sharareh; Theruvath, Johanna; Hutter, Gregor; Azad, Tej D.; Wadi, Lina; Bolin, Sara; Ramaswamy, Vijay; Esparza, Rogelio; Liu, Kun Wei; Edwards, Michael; Swartling, Fredrik J.; Sahoo, Debashis; Li, Gordon; Wechsler-Reya, Robert J.; Reimand, Jüri; Cho, Yoon-Jae; Taylor, Michael D.; Weissman, Irving L.; Mitra, Siddhartha S.; Cheshier, Samuel H.

In: Nature communications, Vol. 9, No. 1, 4121, 01.12.2018.

Research output: Contribution to journalArticle

Kahn, SA, Wang, X, Nitta, RT, Gholamin, S, Theruvath, J, Hutter, G, Azad, TD, Wadi, L, Bolin, S, Ramaswamy, V, Esparza, R, Liu, KW, Edwards, M, Swartling, FJ, Sahoo, D, Li, G, Wechsler-Reya, RJ, Reimand, J, Cho, Y-J, Taylor, MD, Weissman, IL, Mitra, SS & Cheshier, SH 2018, 'Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma', Nature communications, vol. 9, no. 1, 4121. https://doi.org/10.1038/s41467-018-06564-9
Kahn, Suzana A. ; Wang, Xin ; Nitta, Ryan T. ; Gholamin, Sharareh ; Theruvath, Johanna ; Hutter, Gregor ; Azad, Tej D. ; Wadi, Lina ; Bolin, Sara ; Ramaswamy, Vijay ; Esparza, Rogelio ; Liu, Kun Wei ; Edwards, Michael ; Swartling, Fredrik J. ; Sahoo, Debashis ; Li, Gordon ; Wechsler-Reya, Robert J. ; Reimand, Jüri ; Cho, Yoon-Jae ; Taylor, Michael D. ; Weissman, Irving L. ; Mitra, Siddhartha S. ; Cheshier, Samuel H. / Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma. In: Nature communications. 2018 ; Vol. 9, No. 1.
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