Noradrenaline, somatostatin and opioids inhibit activity of single HVA/N-type calcium channels in excised neuronal membranes

Ke-Zhong Shen, A. Surprenant

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Unitary barium currents were measured in outside-out patches of membrane from acutely dissociated guinea-pig submucosal neurones. A single set of HVA/N-like calcium channels with unitary conductance in 110 mM barium of approximately 10 pS was activated by depolarization; channel activity did not show inactivation during 100 ms duration depolarizing pulses. Somatostatin, [Met5]enkephalin and α2-adrenoceptor agonists, which are known to partially inhibit macroscopic calcium currents in these neurones, decreased frequency of single channel opening per depolarizing pulse without altering unitary conductance. Agonists were effective only when GTP was included in the patch pipette. These experiments directly demonstrate that an excised membrane patch can sustain local convergence of multiple receptors to a single calcium channel.

Original languageEnglish (US)
Pages (from-to)614-616
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume418
Issue number6
DOIs
StatePublished - Jul 1991

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N-Type Calcium Channels
Barium
Calcium Channels
Somatostatin
Opioid Analgesics
Neurons
Norepinephrine
Membranes
Enkephalins
Depolarization
Guanosine Triphosphate
Adrenergic Receptors
Guinea Pigs
Calcium
Experiments

ASJC Scopus subject areas

  • Physiology

Cite this

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T1 - Noradrenaline, somatostatin and opioids inhibit activity of single HVA/N-type calcium channels in excised neuronal membranes

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