Noninvasive imaging of myocardial reperfusion injury using leukocyte-targeted contrast echocardiography

Jonathan P. Christiansen, Howard Leong-Poi, Alexander L. Klibanov, Sanjiv Kaul, Jonathan R. Lindner

    Research output: Contribution to journalArticle

    137 Scopus citations

    Abstract

    Background - We hypothesized that myocardial contrast echocardiography (MCE) with leukocyte-targeted microbubbles could temporally and spatially characterize the severity of postischemic myocardial inflammation. Methods and Results - In 9 open-chest dogs, either the left anterior descending or left circumflex coronary artery was occluded for 90 minutes (n=6), while the remaining dogs served as non-ischemic controls. During occlusion, MCE was performed to determine the risk area (RA) and regions supplied by collateral flow. Myocardial inflammation was assessed 5, 60, and 120 minutes after reflow by MCE imaging of leukocyte-targeted (phosphatidylserine-containing) lipid microbubbles. The spatial extent and severity of inflammation were also assessed by radionuclide imaging of the neutrophil-avid tracer 99mTcRP517 and tissue myeloperoxidase activity. Early after reflow, MCE detected inflammation throughout the entire risk area, the extent of which decreased over time due to reduced signal in collateral-supplied regions. The spatial extent of inflammation late after reflow was similar for MCE and radionuclide imaging. The severity of inflammation in the infarct zone, the noninfarcted risk area, and collateral-supplied territories determined by quantitative MCE correlated well with myeloperoxidase activity (r=0.81). Conclusions - MCE with leukocyte-targeted microbubbles can temporally assess the severity and extent of postischemic myocardial inflammation and could be used to evaluate new treatment strategies designed to limit inflammation in acute coronary syndromes.

    Original languageEnglish (US)
    Pages (from-to)1764-1767
    Number of pages4
    JournalCirculation
    Volume105
    Issue number15
    DOIs
    StatePublished - Apr 16 2002

    Keywords

    • Echocardiography
    • Inflammation
    • Schemia

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine
    • Physiology (medical)

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