Noncalcium-dependent modulation of in vitro atrial natriuretic factor release by extracellular osmolality

D. M. Gibbs

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Recent reports have described modulation of atrial natriuretic factor (ANF) secretion by atrial stretch, increased Na+ concentration, and a variety of hormones and neurotransmitters. One problem in the study of ANF secretion has been the isolation of stimulatory effects from interference due to rhythmic myocardial contraction. To avoid this problem, rat atria were dispersed, the myocytes were suspended in a polyacrylamide gel matrix, and the resulting cell column was perifused with a physiological buffer. The secretion of ANF immunoactivity was markedly stimulated by increases in extracellular osmolality, regardless of the solute (NaCl, KCl, or glucose). This effect did not require extracellular Ca2+, nor could it be mimicked by depolarizing concentrations of K+ in an isotonic medium. Functional viability of this model was demonstrated by significant dose-related increases in ANF release in response to as little as 1 nM epinephrine. The dissociation of ANF secretion from depolarization-induced changes in Ca2+ flux is unusual and may represent an adaptation to the dual roles of the atrial myocyte, contraction and secretion.

Original languageEnglish (US)
Pages (from-to)194-197
Number of pages4
JournalEndocrinology
Volume120
Issue number1
StatePublished - 1987

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Atrial Natriuretic Factor
Osmolar Concentration
Muscle Cells
Myocardial Contraction
Epinephrine
Neurotransmitter Agents
Buffers
In Vitro Techniques
Hormones
Glucose

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Noncalcium-dependent modulation of in vitro atrial natriuretic factor release by extracellular osmolality. / Gibbs, D. M.

In: Endocrinology, Vol. 120, No. 1, 1987, p. 194-197.

Research output: Contribution to journalArticle

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