Non-disruptive, real-time analyses of the metabolic status and viability of Streptococcus mutans cells in response to antimicrobial treatments

Justin Merritt, Jens Kreth, Fengxia Qi, Richard Sullivan, Wenyuan Shi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Streptococcus mutans is one of a small number of recognized pathogens that lives among the hundreds of other bacterial species which comprise the oral flora. The virulence of this organism is intimately associated with its ability to live as an attached biofilm community on the tooth surface, and consequently, there is a great interest in its biofilm lifestyle. Currently, there are no established protocols that facilitate drug screening against this organism while it is entrenched in the biofilm. Furthermore, greater complications arise when attempting to perform these experiments in a multi-species setting. In an effort to circumvent these problems, we developed a quick, real-time, and non-disruptive method to probe the metabolic status of S. mutans growing as either a planktonic culture or a biofilm community. This assay takes advantage of the proven utility of luciferase measurements for drug screening. We placed the luciferase gene under the control of the S. mutans lactate dehydrogenase promoter (ldh) and integrated the construct onto its native position on the chromosome. We found this construct to be both highly expressed (

Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalJournal of Microbiological Methods
Volume61
Issue number2
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

Streptococcus mutans
Biofilms
Preclinical Drug Evaluations
Luciferases
Aptitude
L-Lactate Dehydrogenase
Virulence
Life Style
Tooth
Chromosomes
Genes

Keywords

  • Biofilm
  • Lactate dehydrogenase
  • Luciferase
  • Streptococcus mutans

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Non-disruptive, real-time analyses of the metabolic status and viability of Streptococcus mutans cells in response to antimicrobial treatments. / Merritt, Justin; Kreth, Jens; Qi, Fengxia; Sullivan, Richard; Shi, Wenyuan.

In: Journal of Microbiological Methods, Vol. 61, No. 2, 05.2005, p. 161-170.

Research output: Contribution to journalArticle

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AU - Shi, Wenyuan

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