Nitric oxide distribution and production in the guinea pig cochlea

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalHearing Research
Volume153
Issue number1-2
DOIs
StatePublished - 2001

Fingerprint

Outer Auditory Hair Cells
Cochlea
Nitric Oxide
Guinea Pigs
Inner Auditory Hair Cells
Blood Vessels
Spiral Ligament of Cochlea
Basilar Membrane
Stria Vascularis
Organ of Corti
Nerve Endings
Fluorescent Dyes
Nitric Oxide Synthase
Synaptic Transmission
Arginine
Endothelial Cells
Observation

Keywords

  • 4,5-Diaminofluorescein diacetate
  • Endothelial cell
  • L-Arginine
  • N-Monomethyl-L-arginine monoacetate
  • N-Nitro-L-arginine methyl ester HCl
  • Nitric oxide
  • Outer hair cell

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Nitric oxide distribution and production in the guinea pig cochlea. / Shi, Xiao; Ren, Tianying; Nuttall, Alfred.

In: Hearing Research, Vol. 153, No. 1-2, 2001, p. 23-31.

Research output: Contribution to journalArticle

@article{a221bbf7d41249e7a44d85320ba10c5e,
title = "Nitric oxide distribution and production in the guinea pig cochlea",
abstract = "Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.",
keywords = "4,5-Diaminofluorescein diacetate, Endothelial cell, L-Arginine, N-Monomethyl-L-arginine monoacetate, N-Nitro-L-arginine methyl ester HCl, Nitric oxide, Outer hair cell",
author = "Xiao Shi and Tianying Ren and Alfred Nuttall",
year = "2001",
doi = "10.1016/S0378-5955(00)00254-9",
language = "English (US)",
volume = "153",
pages = "23--31",
journal = "Hearing Research",
issn = "0378-5955",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Nitric oxide distribution and production in the guinea pig cochlea

AU - Shi, Xiao

AU - Ren, Tianying

AU - Nuttall, Alfred

PY - 2001

Y1 - 2001

N2 - Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.

AB - Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.

KW - 4,5-Diaminofluorescein diacetate

KW - Endothelial cell

KW - L-Arginine

KW - N-Monomethyl-L-arginine monoacetate

KW - N-Nitro-L-arginine methyl ester HCl

KW - Nitric oxide

KW - Outer hair cell

UR - http://www.scopus.com/inward/record.url?scp=0035133829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035133829&partnerID=8YFLogxK

U2 - 10.1016/S0378-5955(00)00254-9

DO - 10.1016/S0378-5955(00)00254-9

M3 - Article

C2 - 11223294

AN - SCOPUS:0035133829

VL - 153

SP - 23

EP - 31

JO - Hearing Research

JF - Hearing Research

SN - 0378-5955

IS - 1-2

ER -