New markers for tracking endoderm induction and hepatocyte differentiation from human pluripotent stem cells

Audrey Holtzinger, Philip R. Streeter, Farida Sarangi, Scott Hillborn, Maryam Niapour, Shinichiro Ogawa, Gordon Keller

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The efficient generation of hepatocytes from human pluripotent stem cells (hPSCs) requires the induction of a proper endoderm population, broadly characterized by the expression of the cell surface marker CXCR4. Strategies to identify and isolate endoderm subpopulations predisposed to the liver fate do not exist. In this study, we generated mouse monoclonal antibodies against human embryonic stem cell-derived definitive endoderm with the goal of identifying cell surface markers that can be used to track the development of this germ layer and its specification to a hepatic fate. Through this approach, we identified two endoderm-specific antibodies, HDE1 and HDE2, which stain different stages of endoderm development and distinct derivative cell types. HDE1 marks a definitive endoderm population with high hepatic potential, whereas staining of HDE2 tracks with developing hepatocyte progenitors and hepatocytes. When used in combination, the staining patterns of these antibodies enable one to optimize endoderm induction and hepatic specification from any hPSC line.

Original languageEnglish (US)
Pages (from-to)4253-4265
Number of pages13
JournalDevelopment (Cambridge)
Volume142
Issue number24
DOIs
StatePublished - Dec 15 2015

Keywords

  • Endoderm marker
  • Hepatocyte differentiation
  • Human pluripotent stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Holtzinger, A., Streeter, P. R., Sarangi, F., Hillborn, S., Niapour, M., Ogawa, S., & Keller, G. (2015). New markers for tracking endoderm induction and hepatocyte differentiation from human pluripotent stem cells. Development (Cambridge), 142(24), 4253-4265. https://doi.org/10.1242/dev.121020