New genetic tactics to model alveolar rhabdomyosarcoma in the mouse

Charles Keller, Mario R. Capecchi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Using conditional knock-in and knock-out techniques, we designed a mouse model of the childhood muscle cancer alveolar rhabdomyosarcoma (ARMS) that is driven by the chromosomal translocation product, Pax3:Fkhr. Tumors that closely recapitulate the spectrum of molecular markers and histology seen in human ARMS are exclusively produced in this model. Unexpectedly, expression of Pax3:Fkhr in muscle satellite cells did not produce tumors, but it did in differentiating myofibers. Expression of Pax3:Fkhr in muscle is necessary but not sufficient to initiate tumorigenesis at high frequency. This model offers new insight into the roots of alveolar rhabdomyosarcoma and illustrates the utility of Cre-loxP technology for studying otherwise inaccessible cancers in the mouse.

Original languageEnglish (US)
Pages (from-to)7530-7532
Number of pages3
JournalCancer Research
Volume65
Issue number17
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

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Alveolar Rhabdomyosarcoma
Muscle Neoplasms
Neoplasms
Genetic Translocation
Muscle Cells
Histology
Carcinogenesis
Technology
Muscles

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

New genetic tactics to model alveolar rhabdomyosarcoma in the mouse. / Keller, Charles; Capecchi, Mario R.

In: Cancer Research, Vol. 65, No. 17, 01.09.2005, p. 7530-7532.

Research output: Contribution to journalArticle

Keller, Charles ; Capecchi, Mario R. / New genetic tactics to model alveolar rhabdomyosarcoma in the mouse. In: Cancer Research. 2005 ; Vol. 65, No. 17. pp. 7530-7532.
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